Fluorescent imaging of acidic compartments in living cells with a high selective novel one-photon ratiometric and two-photon acidic pH probe

Miao, Fang; Song, Guofen; Sun, Yuming; Liu, Yong; Guo, Fuqiang; Zhang, Weijia; Tian, Minggang; Yu, Xiaoqiang

doi:10.1016/j.bios.2013.05.060

Cited by 62 publications

(14 citation statements)

References 40 publications

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“…Membrane potential Ca 2+ (Hajnóczky et al, 1995) Zn 2+ Sreenath et al, 2011;Liu et al, 2012b;Chyan et al, 2014) thiols H 2 O 2 (Dickinson and Chang, 2008) pH (Sarkar et al, 2016) superoxide (Robinson et al, 2006) membrane potential (Nicholls, 2012) Acidic compartments pH gradient, endocytosis pH Miao et al, 2013;Lv et al, 2014;Chen et al, 2015;Wang et al, 2015;Yapici et al, 2015) thiols (Kand et al, 2015) Ca 2+ (Gilroy and Jones, 1992;Schlatterer et al, 1992) vesicle recycling (Gaffield and Betz, 2007;Li et al, 2009) neurotransmitter release (Gubernator et al, 2009) At resting negative membrane potential (top), the permeable oxonols have a high concentration at the extracellular surface of the PM, and energy transfer from the extracellularly bound FL-WGA (acceptor molecule) is favored. FRET is symbolized as the fluorescent changes are small, and repetitive measurements are needed to obtain a good record of action potentials (for recent reviews see Peterka et al [2011] and St-Pierre et al [2015]).…”

Section: Mitochondrial Matrixmentioning

confidence: 99%

“…Neutralization of the luminal pH by drugs (e.g., monensin) or inhibitors of the H + pumps can be simply measured by the decrease in the fluorescence signal (reviewed in Han and Burgess [2010]). More recently, efforts are ongoing to create new and more selectively targeted probes, with better spectral characteristics, to monitor pH Miao et al, 2013;Lv et al, 2014;Chen et al, 2015;Wang et al, 2015;Yapici et al, 2015) and thiols (Kand et al, 2015). A special case is that of synaptic vesicles that can be visualized using FM1-43, an amphiphilic membraneimpermeable fluorescent dye that inserts into the outer leaflet of the PM, increasing its fluorescence, and is internalized within synaptic vesicles in active neurons during endocytosis (Gaffield and Betz, 2007;Li et al, 2009).…”

Section: Mitochondrial Matrixmentioning

confidence: 99%

See 1 more Smart Citation

Exploring cells with targeted biosensors

Pendin

Greotti

Lefkimmiatis

et al. 2016

Journal of General Physiology

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Cellular signaling networks are composed of multiple pathways, often interconnected, that form complex networks with great potential for cross-talk. Signal decoding depends on the nature of the message as well as its amplitude, temporal pattern, and spatial distribution. In addition, the existence of membrane-bound organelles, which are both targets and generators of messages, add further complexity to the system. The availability of sensors that can localize to specific compartments in live cells and monitor their targets with high spatial and temporal resolution is thus crucial for a better understanding of cell pathophysiology. For this reason, over the last four decades, a variety of strategies have been developed, not only to generate novel and more sensitive probes for ions, metabolites, and enzymatic activity, but also to selectively deliver these sensors to specific intracellular compartments. In this review, we summarize the principles that have been used to target organic or protein sensors to different cellular compartments and their application to cellular signaling.

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Section: Mitochondrial Matrixmentioning

confidence: 99%

Section: Mitochondrial Matrixmentioning

confidence: 99%

Exploring cells with targeted biosensors

Pendin

Greotti

Lefkimmiatis

et al. 2016

Journal of General Physiology

View full text Add to dashboard Cite

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“…The red-shift can be attributed to the introduction of heteroatom and positive charge in fluorescent probes, the interaction force between organic fluorescent dye molecules and the DCM is small in the process of the solvation effect since the DCM is a weak polar aprotic solvent. The solvent with larger polarity could reduce the energy difference between excited state and ground state, which led to a red-shift of the molecule [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix Parvicella In Situ

Jiao

Fei

et al. 2017

Materials

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In this study, two novel fluorescent probes, probe A and probe B were designed, synthesized and characterized, based on Microthrix parvicella (M. parvicella) preferring to utilize long-chain fatty acid (LCFA), for the labeling of M. parvicella in activated sludge. The molecular structure of probe A and probe B include long-chain alkane and LCFA, respectively. The results indicated that probe A and probe B had a large stokes shift of 118 nm and 120 nm and high quantum yield of 0.1043 and 0.1058, respectively, which were significantly helpful for the fluorescent labeling. As probe A was more stable than probe B in activated sludge, and the fluorescence intensity keep stable during 24 h, probe A was more suitable for labeling M. parvicella in situ. In addition, through the Image Pro Plus 6 (IPP 6) analysis, a quantitative relationship was established between sludge volume index (SVI) and integral optical density (IOD) of the labeled M. parvicella in activated sludge samples. The relationship between IOD and SVI conforms to Logistic curve (R2 = 0.94).

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“…So far, a number of excellent pH probes working for weak acidic organelles (such as lysosomes, pH 4.5-5.0) [7][8][9][10][11][12][13][14][15][16][17] or near neutral cytosol (pH 6. 8-7.4) [7,[18][19][20][21][22][23] have been exploited, and some are commercially available [7].…”

Section: Introductionmentioning

confidence: 99%