Fluorescent Gold Nanodots Based Sensor Array for Proteins Discrimination

Yuan, Zhiqin; Du, Yi; Tseng, Yu‐Ting; Peng, Meihua; Cai, Na; He, Yan; Chang, Huan‐Tsung; Yeung, Edward S.

doi:10.1021/ac5045302

Cited by 115 publications

(66 citation statements)

References 49 publications

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“…Dual-ligand cofunctionalized AuNCs were synthesized according to previous reports with slight modification [40, 68]. Firstly, 100 μL of NaOH (1 M) solution and 24 μL of THPC (8%, wt) solution were mixed with 8 mL of ultrapure water under violent stirring for 5 min, and then, 400 μL of HAuCl 4 (Au(III), 24 mM) solution was added rapidly.…”

Section: Methodsmentioning

confidence: 99%

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Tao

Auguste

2017

Biomaterials

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Early detection of breast cancer is a critical component in patient prognosis and establishing effective therapy regimens. Here, we developed an easily accessible yet potentially powerful sensor to detect cancer cell targets by utilizing seven dual-ligand cofunctionalized gold nanoclusters (AuNCs) as both effective cell recognition elements and signal transducers. On the basis of this AuNC multichannel sensor, we have successfully distinguished healthy, cancerous and metastatic human breast cells with excellent reproducibility and high sensitivity. Triple negative breast cancer cells (TNBCs), which exhibit low expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2, were identified. The high accuracy of the blind breast cell sample tests further validates the practical application of the sensor array. In addition, the versatility of the sensor array is further justified by identifying amongst distinct cell types, different cell concentrations and cell mixtures. Notably, the drug-resistant cancer cells can also be efficiently discriminated. Furthermore, the dual-ligand cofunctionalized AuNCs can efficiently differentiate different cells from the peripheral blood of tumor-free and tumor-bearing mice. Taken together, this fluorescent AuNCs based array provides a powerful cell analysis tool with potential applications in biomedical diagnostics.

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Section: Methodsmentioning

confidence: 99%

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Tao

Auguste

2017

Biomaterials

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“…Häufig kombinierten die Arbeiten einen fluorogenen Sensor mit einer Auswahl von Erkennungselementen, die unterschiedliche Fluoreszenzmuster generieren, wenn sie an das Zielprotein in der Lösung gebunden/nicht gebunden sind. Eine hervorragender Konzeptbeweis war die erfolgreiche Detektion einer großen Vielfalt verschiedener Proteine unter Anwendung von fluoreszierenden Metallkomplexen, Nanoclustern, Quantenpunkten oder kolorimetrischer Gold‐Nanopartikel‐Aggregation . Dennoch muss festgehalten werden, dass – während es viele Beispiele für Array‐basierte Sensorik in Puffer oder Wasser gibt – nur relativ wenige Gruppen ihre Systeme in biologisch relevanten Medien getestet haben.…”

Section: Array‐basierte Sensoren Für Biomedizinische Anwendungenunclassified

Array‐basierte Sensorik mit der “chemischen Nase” in der Diagnostik und Wirkstoffentdeckung

2019

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Array‐basierte Sensorplattformen – “Chemische Nasen/Zungen” – sind inspiriert vom olfaktorischen System von Säugetieren. Multiple Sensorelemente in diesen Geräten interagieren selektiv mit Zielanalyten und produzieren dabei ein spezifisches Antwortmuster, wodurch sie die Identifikation von Analyten ermöglichen. Dieser Ansatz bietet einzigartige Möglichkeiten im Vergleich mit “traditionellen”, hoch‐spezifischen Sensorelementen, z. B. Antikörpern. Array‐basierte Sensoren sind herausragend, wenn es um die Identifikation kleiner Veränderungen in komplexen Mischungen geht, und ebendiese Fähigkeit wird nun in der chemischen Biologie und klinischen Pathologie genutzt, ermöglicht durch ein vielfältiges Instrumentarium neuer Molekular‐, Biokonjugat‐ und Nanomaterial‐Techniken. Innovationen beim Design und der Analyse der Arrays stellen ein robustes Instrumentarium für moderne biomedizinische Zielsetzungen, einschließlich der Präzisionsmedizin, zur Verfügung.

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“…Recently, Yuan and coworkers synthesized eight dual-ligand cofunctionalized AuNCs by using the one-pot and two-step strategy. They constructed an array-based protein discrimination system by using these eight AuNCs as efficient protein receptors and competent signal transducers [113]. On the basis of this sensor array, eight proteins were well distinguished at low concentration (A 280 = 0.005) by analyzing the fluorescence response patterns.…”

Section: Detection Of Proteinsmentioning

confidence: 99%

Fluorescent Gold Nanoclusters: Promising Fluorescent Probes for Sensors and Bioimaging

Wang

Zhu

2017

J. Anal. Test.

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Fluorescent gold nanoclusters (AuNCs) have recently emerged as a novel kind of promising fluorescent probes for high-performance sensors and bioimaging because of their ultrasmall size (\3 nm), strong luminescence, good photostability, and excellent biocompatibility. Over the past decade, we have witnessed growing popularity of AuNCs in analytical applications and enormous efforts have been devoted to their development. In this review, we provide an update on recent advances in the development of AuNCs in terms of physicochemical properties, synthesis strategies, and bioapplications. The optical, electrochemical, catalytical, and solvatochromic properties of AuNCs are first summarized, which are followed by different ligands or template-assisted controllable synthetic methods. Afterwards functionalization of AuNCs is described in terms of ligand exchange, bioconjugation, and noncovalent interaction. We then focus on the applications of AuNCs as fluorescent probes for detection of metal ions, inorganic anions, small biomolecules, proteins, nucleic acids, drug molecules, pH, and temperature. We also summarize the usage of metal NCs in cellular and in vivo targeting and imaging. Finally, we conclude with a brief look at the future challenges and prospects of the development of AuNCs.

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Fluorescent Gold Nanodots Based Sensor Array for Proteins Discrimination

Cited by 115 publications

References 49 publications

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Array‐basierte Sensorik mit der “chemischen Nase” in der Diagnostik und Wirkstoffentdeckung

Fluorescent Gold Nanoclusters: Promising Fluorescent Probes for Sensors and Bioimaging

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