Fluorescent fibre‐optic confocal imaging of lesional and non‐lesional psoriatic skin compared with normal skin <i>in vivo</i>

Suihko, Christian; Serup, Jørgen

doi:10.1111/j.1600-0846.2011.00585.x

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…For example, genomic, proteomic, and lipidomic analyses of human wounds have already improved our understanding of the mechanisms that guide the repair process and its impairment, and have identified several potential biomarkers, such as c-myc and β-catenin (7, 178), S100 proteins and MMPs (48), or glycerophosphocholines, glycerophosphoglycerols, glycerophosphoinositols, and triacylglycerols (181). Similarly, portable confocal imaging, which can provide noninvasive assessment of lesional and nonlesional psoriatic skin (182), is revolutionizing diagnosis and treatment of this disorder. With new initiatives in “big data,” one can envision the integration of genomic, proteomic, and microbiome data, as well as nanomedicine and imaging merging with electronic medical records to formulate patient-specific treatment plans.…”

Section: Unanswered Questions and Emerging Focus Areasmentioning

confidence: 99%

Wound repair and regeneration: Mechanisms, signaling, and translation

2014

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The cellular and molecular mechanisms underpinning tissue repair and its failure to heal are still poorly understood, and current therapies are limited. Poor wound healing after trauma, surgery, acute illness, or chronic disease conditions affects millions of people worldwide each year and is the consequence of poorly regulated elements of the healthy tissue repair response, including inflammation, angiogenesis, matrix deposition, and cell recruitment. Failure of one or several of these cellular processes is generally linked to an underlying clinical condition, such as vascular disease, diabetes, or aging, which are all frequently associated with healing pathologies. The search for clinical strategies that might improve the body’s natural repair mechanisms will need to be based on a thorough understanding of the basic biology of repair and regeneration. In this review, we highlight emerging concepts in tissue regeneration and repair, and provide some perspectives on how to translate current knowledge into viable clinical approaches for treating patients with wound-healing pathologies.

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Section: Unanswered Questions and Emerging Focus Areasmentioning

confidence: 99%

Wound repair and regeneration: Mechanisms, signaling, and translation

2014

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“…Moreover, in a comparative study of 12 psoriasis patients and eight healthy controls, a combination of topical application and subsequent subepidermal injections of fluorescein sodium characteristic features of keratinocytes in lesional as well as nonlesional psoriatic skin different from healthy skin was demonstrated. The horizontal width of dermal papillae of psoriatic skin was increased by approximately 50% when compared with healthy controls . Overall, a good visualization of cell nuclei and parakeratosis was demonstrated in psoriatic epidermis, and in FCM images of skin with fluorescein the contrast to normal skin was increased compared with RCM images.…”

Section: Resultsmentioning

confidence: 81%

Potential of contrast agents to enhance in vivo confocal microscopy and optical coherence tomography in dermatology: A review

Ring

Israelsen

Bang

et al. 2019

Journal of Biophotonics

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Distinction between normal skin and pathology can be a diagnostic challenge. This systematic review summarizes how various contrast agents, either topically delivered or injected into the skin, affect distinction between skin disease and normal skin when imaged by optical coherence tomography (OCT) and confocal microscopy (CM). A systematic review of in vivo OCT and CM studies using exogenous contrast agents on healthy human skin or skin disease was performed. In total, nine CM studies and one OCT study were eligible. Four contrast agents aluminum chloride (AlCl) n = 2, indocyanine green (ICG) n = 3, sodium fluorescein n = 3 and acetic acid n = 1 applied to CM in variety of skin diseases. ICG, acetic acid and AlCl showed promise to increase contrast of tumor nests in keratinocyte carcinomas. Fluorescein and ICG enhanced contrast of keratinocytes and adnexal structures. In OCT of healthy skin gold nanoshells, increased contrast of natural skin openings. Contrast agents may improve delineation and diagnosis of skin cancers; ICG, acetic acid and AlCl have potential in CM and gold nanoshells facilitate visualization of adnexal skin structures in OCT. However, as utility of bedside optical imaging increases, further studies with robust methodological quality are necessary to implement contrast agents into routine dermatological practice.

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“…FCFM has been widely used in mice, for drug and nanoparticle distribution studies [ 16 – 18 ], tumor angiogenesis imaging [ 19 ], DNA fragmentation visualization [ 20 ], and the diagnosis of cancer and infections [ 21 – 23 ]. FCFM has also been used to examine the microstructural organization of various tissues in humans, including the skin [ 24 , 25 ], cervix [ 26 ], and gastrointestinal tract [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques

Todorova

Salabert

Tricot

et al. 2017

Contrast Media & Molecular Imaging

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Purpose We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates. Procedures Macaques were intradermally injected with a monoclonal, fluorescently labeled antibody against HLA-DR molecule and were imaged for up to 5 days by fibered confocal microscopy (FCFM). Results The network of skin Langerhans cells was visualized by in vivo fibered confocal fluorescence microscopy. Quantification of Langerhans cells revealed no changes to cell density with time. Ex vivo experiments confirmed that injected fluorescent HLA-DR antibody specifically targeted Langerhans cells in the epidermis. Conclusions This study demonstrates the feasibility of single-cell, in vivo imaging as a noninvasive technique to track Langerhans cells in nontransgenic animals.

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Fluorescent fibre‐optic confocal imaging of lesional and non‐lesional psoriatic skin compared with normal skin in vivo

Cited by 4 publications

References 3 publications

Wound repair and regeneration: Mechanisms, signaling, and translation

Wound repair and regeneration: Mechanisms, signaling, and translation

Potential of contrast agents to enhance in vivo confocal microscopy and optical coherence tomography in dermatology: A review

Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques

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