Fluorescent dye-doped silica nanoparticles: new tools for bioapplications

Bae, Se Won; Tan, Weihong; Hong, Jong‐In

doi:10.1039/c2cc16306c

Cited by 213 publications

(139 citation statements)

References 121 publications

(109 reference statements)

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“…A large number of dyes have been conjugated to silica nanoparticles, primarily by covalent linkage, and used in biological research for imaging and targeting specific cells by appropriate surface modification of the silica. 30 There are also reports of bioanalysis and biodetection using fluorescently labeled silica particles. 30 Different synthesis protocols are reported, but most studies do not report QY values for bound dye, which would allow for direct comparison of the syntheses.…”

Section: Discussionmentioning

confidence: 99%

“…30 There are also reports of bioanalysis and biodetection using fluorescently labeled silica particles. 30 Different synthesis protocols are reported, but most studies do not report QY values for bound dye, which would allow for direct comparison of the syntheses. In addition to brightness, the stability of the particles and their ability to retain dyes for certain applications is crucial.…”

Section: Discussionmentioning

confidence: 99%

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Uptake of bright fluorophore core-silica shell nanoparticles by biological systems

Zane¹,

McCracken²,

Knight³

et al. 2015

IJN

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Nanoparticles are used in a variety of consumer applications. Silica nanoparticles in particular are common, including as a component of foods. There are concerns that ingested nano-silica particles can cross the intestinal epithelium, enter the circulation, and accumulate in tissues and organs. Thus, tracking these particles is of interest, and fluorescence spectroscopic methods are well-suited for this purpose. However, nanosilica is not fluorescent. In this article, we focus on core-silica shell nanoparticles, using fluorescent Rhodamine 6G, Rhodamine 800, or CdSe/CdS/ZnS quantum dots as the core. These stable fluorophore/silica nanoparticles had surface characteristics similar to those of commercial silica particles. Thus, they were used as model particles to examine internalization by cultured cells, including an epithelial cell line relevant to the gastrointestinal tract. Finally, these particles were administered to mice by gavage, and their presence in various organs, including stomach, small intestine, cecum, colon, kidney, lung, brain, and spleen, was examined. By combining confocal fluorescence microscopy with inductively coupled plasma mass spectrometry, the presence of nanoparticles, rather than their dissolved form, was established in liver tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Uptake of bright fluorophore core-silica shell nanoparticles by biological systems

Zane¹,

McCracken²,

Knight³

et al. 2015

IJN

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“…One material that may prove useful in combining a dual imaging and therapy is mesoporous silica NPs; with their large surface areas and pore volumes, one or two modalities (optical imaging agent and an anticancer drug) can be incorporated into the silica matrix while loading the other modality into its pores. [509][510][511] Encapsulating drug payloads in NPs can prevent exposure of healthy cells to the cytotoxic drug and may prove more beneficial (e.g., lower toxicity and fewer side effects) at lower doses than the free drug. However, this can reveal much more complex than the use of simple small-molecule drugs that are easily characterized.…”

Section: Perspectives: Multimodal Theranostic Npsmentioning

confidence: 99%

Functionalized nanomaterials: their use as contrast agents in bioimaging: mono- and multimodal approaches

Trequesser¹,

Seznec²,

Delville³

2016

Nano Online

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The successful development of nanomaterials illustrates the considerable interest in the development of new molecular probes for medical diagnosis and imaging. Substantial progress was made in synthesis protocol and characterization of these materials whereas toxicological issues are sometimes incomplete. Nanoparticle-based contrast agents tend to become efficient tools for enhancing medical diagnostics and surgery for a wide range of 2 imaging modalities. Multimodal nanoparticles (NPs) are much more efficient than conventional molecular-scale contrast agents. They provide new abilities for in vivo detection and enhanced targeting efficiencies through longer circulation times, designed clearance pathways, and multiple binding capacities. Properly protected, they can safely be used for the fabrication of various functional systems with targeting properties, reduced toxicity and proper removal from the body. This review mainly describes the advances in the development of mono-to multimodal NPs and their in vitro and in vivo relevant biomedical applications ranging from imaging and tracking to cancer treatment. Besides specific applications for classical imaging, (MRI, PET, CT, US, PAI) are also mentioned less common imaging techniques such as terahertz molecular imaging (THMI) or ion beam analysis (IBA).Perspectives on multimodal theranostic NPs and their potential for clinical advances are also mentioned.

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“…28 In this study, monodisperse 50 nm spherical SiNPs were chosen as starting material to generate biocompatible multifunctional nanoconjugates. First, in order to have a fluorescent detectable carrier, the dense core of the SiNPs was loaded with a fluorophore, which was protected by an external denser silica shell necessary to prevent the undesired cargo release and limit the dye bleaching that often leads to misleading optical artifacts.…”

Section: Synthesis and Characterization Of Sinpsmentioning

confidence: 99%

Development of<sup> 99m</sup>Tc-radiolabeled nanosilica for targeted detection of HER2-positive breast cancer

Rainone¹,

Riva²,

Belloli³

et al. 2017

IJN

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The human epidermal growth factor receptor 2 (HER2) is normally associated with a highly aggressive and infiltrating phenotype in breast cancer lesions with propensity to spread into metastases. In clinic, the detection of HER2 in primary tumors and in their metastases is currently based on invasive methods. Recently, nuclear molecular imaging techniques, including positron emission tomography and single photon emission computed tomography (SPECT), allowed the detection of HER2 lesions in vivo. We have developed a 99m Tc-radiolabeled nanosilica system, functionalized with a trastuzumab half-chain, able to act as drug carrier and SPECT radiotracer for the identification of HER2-positive breast cancer cells. To this aim, nanoparticles functionalized or not with trastuzumab half-chain, were radiolabeled using the 99m Tc-tricarbonyl approach and evaluated in HER2 positive and negative breast cancer models. Cell uptake experiments, combined with flow cytometry and fluorescence imaging, suggested that active targeting provides higher efficiency and selectivity in tumor detection compared to passive diffusion, indicating that our radiolabeling strategy did not affect the nanoconjugate binding efficiency. Ex vivo biodistribution of 99m Tc-nanosilica in a SK-BR-3 (HER2 + ) tumor xenograft at 4 h postinjection was higher in targeted compared to nontargeted nanosilica, confirming the in vitro data. In addition, viability and toxicity tests provided evidence on nanoparticle safety in cell cultures. Our results encourage further assessment of silica 99m Tc-nanoconjugates to validate a safe and versatile nanoreporter system for both diagnosis and treatment of aggressive breast cancer.

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Fluorescent dye-doped silica nanoparticles: new tools for bioapplications

Cited by 213 publications

References 121 publications

Uptake of bright fluorophore core-silica shell nanoparticles by biological systems

Uptake of bright fluorophore core-silica shell nanoparticles by biological systems

Functionalized nanomaterials: their use as contrast agents in bioimaging: mono- and multimodal approaches

Development of<sup> 99m</sup>Tc-radiolabeled nanosilica for targeted detection of HER2-positive breast cancer

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