Fluorescent Detection Principles and Strategies

Molecular engineering of ruthenium(ii) complexes with (3-polyamino)phenanthroline ligands for developing reusable optical sensors for Cu(ii) ions

“…1,2 This now mature field continues to attract an increasing amount of attention due to rapid progress in instrumentation and steadily growing analytical demands in the field of biochemistry, medicinal chemistry, industrial production and environmental pollution monitoring. 3–7 In this context, special interest is devoted to the development of selective chemosensors and materials for transition metal ions detection, since some of these cations are used in trace amounts in biological processes by living matter, while at the same time they continue to be an environmental and health concern when their concentrations exceed critical levels. 8–10…”

Synthesis of Macrocyclic Receptors with Intrinsic Fluorescence Featuring Quinizarin Moieties

“…1 Thereby, the quality of the complexation equilibrium is indicated by a change in the intensity of the emitted light. 2 In many cases, the used sensor-active materials consist of a macrocycle connected to a pendant fluorophore. Much less studied are examples, where the fluorophore is part of the recognition unit.…”

“…Respective sensors have been employed to detect a wide range of chemical and biochemical species as for example cations, anions, neutral molecules, biochemical analytes, and gases . Thereby, the quality of the complexation equilibrium is indicated by a change in the intensity of the emitted light . In many cases, the sensor-active materials used consist of a macrocycle connected to a pendant fluorophore.…”

Short Wavelength Inner Filter Technique (SWIFT) in Designing Reactive Fluorescent Molecular Probes

“…In the last few decades, the development of reactive fluorescent molecular probes has become an extremely popular research area. , The majority of such probes consist of covalently connected reporting and reactive units, with a chemical reaction between the latter and analyte leading to a change in the fluorescence intensity of the reporting unit. , However, an argument can be made that chemical attachment of these two units is advantageous only when their presence at the same place and time is required by the application, e.g., in vivo studies. In the absence of this requirement, the chemical connection adds to the cost of the probes and limits their development to those bearing chemical groups appropriate for or compatible with such connection.…”