Fluorescent Detection of Apoptotic Cells by Using Zinc Coordination Complexes with a Selective Affinity for Membrane Surfaces Enriched with Phosphatidylserine

Hanshaw, Roger G.; Lakshmi, C.; Lambert, Timothy N.; Johnson, James R.; Smith, Bradley D.

doi:10.1002/cbic.200500149

Cited by 92 publications

(99 citation statements)

References 32 publications

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“…Other novel approaches include the small-molecule zinc-dipicolylamine coordination complex 74 and cationic liposmes. 75 There is also a report describing a twelve-residue PS-binding peptide discovered by screening a phage library of random peptides.…”

Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

In vivo imaging of apoptosis

Blankenberg¹

2008

Cancer Biology & Therapy

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Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

In vivo imaging of apoptosis

Blankenberg¹

2008

Cancer Biology & Therapy

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“…PS is primarily observed on the inner surface of the lipid bilayer of the plasma membrane facing the cytosol. The plasma membrane of cells that are undergoing apoptosis will have a break up of the phospholipid asymmetry and expose PS on the surface, which an indicator of early apoptosis [14]. The cells evaluated after longer post-PHT times were positive for PI, indicating the presence of a loss of membrane integrity, which provided greater access to the inside of the cells [36].…”

Section: Discussionmentioning

confidence: 99%

Morphological Study in B16F10 Murine Melanoma Cells after Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG)

Radzi

Osaki

Tsuka

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. Photodynamic hyperthermal therapy (PHT) with indocyanine green (ICG) is a combination of photodynamic therapy (PDT) and hyperthermia (HT). The low toxicity of ICG with an absorption wavelength of 700-800 nm is thought to make it a good candidate as a photosensitizer for PHT. Upon irradiation, ICG produces oxygen radicals and generates heat. The optimal concentration of ICG and the PHT post-irradiation time effects were evaluated by the cytotoxicity of the treatment on B16F10 murine melanoma. The cytotoxicity of PHT was determined based on the morphology of apoptotic and necrotic cells under phase-contrast microscope, confocal laser scanning microscope (CLSM) with DAPI and Annexin V-FITC/PI double staining, and cell surface structure evaluation by scanning electron microscopy (SEM). The use of ICG at a concentration of 150 µM was selected, as cell proliferation was inhibited from 0 to 24 hr post-PHT with a 3-fold decrease in cell viability (P<0.001) compared to the control group. A morphological observation revealed apoptotic and some degree of necrotic features in the PHT-treated cells.

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“…One of these compounds, Zn 2+ -dipicolylamine, binds phosphatidylserine-rich membranes in a Ca 2+ -independent manner and could be a very useful reagent for detecting apoptosis (49,50). In addition, a novel family of low molecular weight compounds based on sulfonamides (ApoSense molecules) has been developed (42, 51, 52).…”

Section: Specific Targets and Imaging Probesmentioning

confidence: 99%

Nuclear Imaging Probes: from Bench to Bedside

Wester

2007

Clinical Cancer Research

101

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The availability of specific imaging probes is the nuclear fuel for molecular imaging by positron emission tomography and single-photon emission computed tomography. These two radiotracer-based imaging modalities represent the prototype methods for noninvasive depiction and quantification of biochemical processes, allowing a functional characterization of tumor biology. A variety of powerful radiolabeled probesötracersöare already established in the routine clinical management of human disease and others are currently subject to clinical assessment. Emerging from investigations of the genomic and proteomic signatures of cancer cells, an increasing number of promising targets are being identified, including receptors, enzymes, transporters, and antigens. Corresponding probes for these newly identified targets need to be developed and transferred into the clinical setting. Starting with a brief summary of the characteristics and prerequisites for a ''good tracer,'' an overview of tracer concepts, target selection, and development strategies is given. The influence of the imaging concepts on tracer development is also discussed.

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Fluorescent Detection of Apoptotic Cells by Using Zinc Coordination Complexes with a Selective Affinity for Membrane Surfaces Enriched with Phosphatidylserine

Cited by 92 publications

References 32 publications

In vivo imaging of apoptosis

In vivo imaging of apoptosis

Morphological Study in B16F10 Murine Melanoma Cells after Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG)

Nuclear Imaging Probes: from Bench to Bedside

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