Fluorescent competitive flow-through assay for chloramphenicol using molecularly imprinted polymers

Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003

“…941 The use of fluorescent tags can considerably lower detection limits as has been demonstrated in the displacement of fluorescein-labelled triazines from triazine 1217 and atrazine 653 MIPs and dansylated chloramphenicol from a chloramphenicol MIP. 1026 A similar approach has been employed with 17-estradiol MIPs 1218 and a fluorescent analogue based on coumarin was used as a fluorescent probe in displacement from a 2,4-D-MIP. 242 The optimization of polymer and displacer in fluorometric assays for -lactam antibiotics has also been discussed, 1219 and the use of non-specific fluorescent dyes in displacement studies has been reported.…”

“…The method of competitive displacement has also been extended to tracking enantioselective MIP/analyte binding events, 1008 and development of a flow-through assay for the antibiotic chloramphenicol. 1026 Rachkov and colleagues 1218 similarly used a competitive displacement system for determination of -estradiol; however, as the template here is intrinsically fluorescent, direct measurement of the analyte concentration could also be made. A commonly used method of introducing fluorescence transduction of binding events to a MIP is derivatization of functional monomers to include a fluorescent moiety, such that when analyte rebinds to a specific cavity wherein these modified monomers are located, resultant changes in the electronic properties of the monomers produce a fluorescent signal.…”

Zearalenone sensing with molecularly imprinted polymers and tailored fluorescent probes

“…As it has been pointed out previously [27], this variable has an important effect on the assay performance. Higher tracer concentrations provided wider dynamic ranges but at the cost of a lower sensitivity so that a 5 × 10 −6 M PARA concentration in the mobile phase was selected for assay optimization.…”

“…A 0.125 mL min −1 flow rate was used to facilitate retention of the tracer in the polymer and to avoid back pressure problems. Then, 1 mL of a 5 × 10 −4 M solution of CDHB (the template molecule), spiked with the fluorescent tracer in order to keep constant the tracer concentration in the carrier stream [27], was injected into the system. Once the CDHB molecules reach the MIP particles, the polymer-bound tracer molecules are displaced by the incoming analyte, originating a decrease in the fluorescence signal of the sensing layer (Fig.…”

Flow-Injection Preconcentration of Chloramphenicol Using Molecularly Imprinted Polymer for HPLC Determination in Environmental Samples

“…Two other particular applications of MIP for CAP were reported in flow injection analysis (FIA) with luminescence detections. In fluorescent competitive flow through assay the MIP was packed to the spectrofluorimetric cell and the limit of detection (LOD) in such system was evaluated as 8 mg/L [24]. The FIA determination of CAP involving online preconcentration was recently reported in microfluidic system with chemiluminescence detection with unprecedented LOD level 2.4 ng/L for analyses in 50  μ L samples [25].…”