A novel fluorescent glucose probe, CdTe/CdS quantum dots (QDs)-glucose oxidase (GOx) complex, was synthesized by the chemical conjugation of aqueous CdTe/CdS QDs and GOx. The concentration of glucose is related to the quenching of the fluorescent CdTe/CdS QDs, which is caused by the production of hydrogen peroxide under the catalysis of GOx. Fluorescence spectrophotometry, UV-vis absorption spectroscopy and transmission electron microscopy were used to characterize the CdTe/CdSQDs-GOx complex. The optimum catalytic pH and temperature of the CdTe/CdS QDs-GOx complex were determined to be 7 and 40 C, respectively. A relation between the fluorescence intensity of the CdTe/CdS QDs-GOx complex and the glucose concentrations was found based on a modified Stern-Volmer equation.