Fluorescent aptaswitch for chloramphenicol detection – Quantification enabled by immobilization of aptamer

Sharma, Ritu; Akshath, Uchangi Satyaprasad; Bhatt, Praveena; Raghavarao, K.S.M.S.

doi:10.1016/j.snb.2019.03.093

Cited by 47 publications

(15 citation statements)

References 34 publications

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“…The effectiveness of aptasensors using fluorescent-labeled probes has been confirmed in many experimental studies. Sharma constructed an aptaswitch sensor complex using a combination of fluorophore and quenching labeled oligonucleotides and an aptamer that recognizes chloramphenicol [92,93]. Aptamer fluorescence detection uses the specific recognition of an aptamer and target antibiotics to regulate the energy transfer efficiency between a fluorescent donor and a recipient, to achieve quantitative detection of target antibiotics through changes in the fluorescence intensity or polarization [94,95].…”

Section: Fluorescent Aptamer Sensorsmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research.

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Section: Fluorescent Aptamer Sensorsmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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“…The presence of the MC-LR target results in the uptake of the aptamer from the dsDNA–CuNCs; therefore, the fluorescent signal exhibits a sensitivity range of 0.01 to 1000 μg/L and LOD of 4.8 ng/L [ 162 ]. Sharma et al provided a technique for chloramphenicol analyte detection using three oligonucleotide complexes comprising an aptamer, fluorophore oligomer, and oligomer quencher as a sensor platform ( Figure 8 F), with a linear range of 10–107 pg/mL and a LOD of 0.987 pg/mL [ 163 ].…”

Section: Fluorescence Aptasensormentioning

confidence: 99%

“…( E ) TTX assay based on the difference in fluorescence response of the berberine reporter, reproduced from [ 160 ]. ( F ) Turn-on fluorescence aptasensor assay for chloramphenicol based on oligomer quencher release, reproduced from [ 163 ].…”

Section: Fluorescence Aptasensormentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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“…on the consumers. Thus many countries including China, United States and European Union have prohibited its use in aquaculture and animal husbandry Sharma et al, 2019). TAP is an analog of CAP in which the nitro group is replaced with a methylsulfonyl group, and FF is a fluorinated analog of TAP (Figure 1), thus FF and TAP have been used as the alternatives to CAP (Fodey et al, 2013;Hu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of three amphenicol antibiotics in shrimp and surface water samples by LC–MS/MS using two-antibodies-immobilized immunoaffinity clean-up technique

Guo

LinChuan

et al. 2021

Food and Agricultural Immunology

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Fluorescent aptaswitch for chloramphenicol detection – Quantification enabled by immobilization of aptamer

Cited by 47 publications

References 34 publications

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Recent Advances in Aptamer Sensors

Simultaneous detection of three amphenicol antibiotics in shrimp and surface water samples by LC–MS/MS using two-antibodies-immobilized immunoaffinity clean-up technique

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