Fluorescent Antibody Tests for Detection of the Gonococcus in Women

Deacon, Wilbur E.; Peacock, William L.; Freeman, Elizabeth M.; Harris, Ad; Bunch, W. L.

doi:10.2307/4590742

Cited by 46 publications

(12 citation statements)

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“…A fluorescent antibody technique has been found to be an effective and rapid method for the detection of N. gonorrhoeae in the female (8). This study was designed to explore the usefulness of the fluorescent antibody method for detecting N. gonorrhoeae in females who had no signs or symptoms indicating gonorrheal infection and who had not been named as sexual contacts of male gonorrhea patients.…”

mentioning

confidence: 99%

Fluorescent Antibody Method of Detecting Gonorrhea in Asymptomatic Females

Harris¹,

Deacon²,

Tiedemann³

et al. 1961

Public Health Reports (1896-1970)

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confidence: 99%

Fluorescent Antibody Method of Detecting Gonorrhea in Asymptomatic Females

Harris¹,

Deacon²,

Tiedemann³

et al. 1961

Public Health Reports (1896-1970)

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“…Also in view of the experience of Deacon et al (1960) and Moore et al (1963), it seems possible th a t, in cases in which the direct results are entirely negative, the delayed FA technique m ay nevertheless produce a num ber of positive results after 16-24 h. Systematic studies in this direction have so far not been made in our laboratory.…”

Section: Resultsmentioning

confidence: 75%

“…Although the data presented by Deacon et al (1960) and Moore et al (1963) indicate th a t the delayed FA technique ensures a diagnosis in a num ber of cases of gonorrhoea which otherwise would be overlooked, a degree of uncertainty nevertheless persists in some cases with a negative culture and positive FA findings.…”

Section: Resultsmentioning

confidence: 99%

“…The reports in the literature (Deacon et al, 1960;Thayer and Moore, 1964) which mentioned a higher sensitivity of the delayed FA tech nique as compared with the culture techniques and the direct FA technique, failed to mention the possibility of aspecificity. Although the data presented by Deacon et al (1960) and Moore et al (1963) indicate th a t the delayed FA technique ensures a diagnosis in a num ber of cases of gonorrhoea which otherwise would be overlooked, a degree of uncertainty nevertheless persists in some cases with a negative culture and positive FA findings.…”

Section: Resultsmentioning

confidence: 99%

“…Material for examination was obtained from the patients with swabs, and smears were immediately made on a nutrient medium pre-heated to 37° C and four slides. The medium used was GC Agar Base with Haemoglobin and Supplement B (DIFCO), which was also employed by Deacon et al (1960), with incubation at 37°C in an atmosphere of 10% C02 during a maximum of 72 h. Two slides were used for Gram and methylene blue staining, while the other two were used for the FA technique (see below). The methylene blue stain was made in order to facilitate the study of the morphology of intracellular diplococci.…”

Section: Methodsmentioning

confidence: 99%

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Diagnosis of Gonorrhea by a Fluorescent Antibody Technique

Mouton¹

1966

Dermatology

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Fluorescent Antibody Techniques

Kendrick¹

1961

Am J Dis Child

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The diagnosis of early, mild, or atypical whooping cough is dependent upon identification of the etiologic agent. Although procedures for the isolation of Bordetella pertussis1 have been available for many years and are of proven diagnostic aid, they have not gained the wide usage they seem to deserve. A recognized limitation is the time needed: 3, more often 4, and occasionally more, days are required for a positive report. The possibility of using fluorescent antibody methods for rapid identification of B. pertussis in nasopharyngeal specimens is suggested by the promising results reported in recent studies of a number of bacterial infections. For example, Group A streptococci,2,3 enteropathogenic Escherichia coli,4 Haemophilus influenzae, Type B,5 Neisseria gonorrhoeae,6 Vibrio comma,7 Leptospira,8 and certain other bacterial agents have been demonstrated by means of this technique in specimens taken directly from the patient. The work of de Repentigny and Frappier,9 although not concerned with diagnosis, indicated that specific antigens of B. pertussis could be stained with dye-conjugated antibody. In a recent diagnostic study by Donaldson and Whitaker,10 31 of 36 nasopharyngeal specimens from infants with symptoms of clinical whooping cough were found positive with the fluorescent antibody technique ; cultural results were not given.In the present study, preliminary tests with an available pertussis antiserum conju¬ gated with fluorescein isothiocyanate showed brilliant fluorescence in slide preparations of several strains of B. pertussis; fluorescence was not observed with a number of cultures

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Fluorescent Antibody Tests for Detection of the Gonococcus in Women

Cited by 46 publications

References 1 publication

Fluorescent Antibody Method of Detecting Gonorrhea in Asymptomatic Females

Fluorescent Antibody Method of Detecting Gonorrhea in Asymptomatic Females

Diagnosis of Gonorrhea by a Fluorescent Antibody Technique

Fluorescent Antibody Techniques

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