Fluorescent Antibody Staining in the Serodiagnosis of Trichinosis

Abstract

A fluorescent staining procedure for Trichinella spiralis and the appearance of the stained larvae are described. The applicability of the method to the sero-diagnosis of trichinosis was investigated. The results obtained both with the experimental and human sera agreed well with the complement-fixation results. In titrating 9 experimental sera and 36 sera from parasitologically proved or clinically diagnosed cases of trichinosis in humans, higher titers were obtained by the immunofluorescent staining, indicating that this test is somewhat more sensitive.

Molecules on the Surface of Parasitic Nematodes as Probes of the Immune Response in Infection

“…In summary, these studies of cell adherence to parasites mediated by antibody have brought to the forefront the importance of the nematode surface in immune rejection as suggested by earlier workers {Soulsby 1963, Taffs & Voller 1962, Crandall et al 1963, Baratawidjaja et al 1963, Sadun 1963. This led us to analyze the surface in far greater depth, and to finally exclude the possibility of the cuticle being an inert, unobtrusive covering provoking little immune responsiveness.…”

The Sites of Antigen Formation in Different Developmental Stages of Clonorchis Sinensis

“…Cuticular fluorescence indicating antigenantibody complexes has been reported in sections of schistosome adults (Andrade, Paronetto and Popper, 1961;Lichtenberg, 1964), Ascaris larvae (Crandall, Echevarria and Arean, 1963) and adult Fasciola (Thorpe, 1965) just as in the Clonorchis adults shown in the present study. When the cuticle of T. spiralis is isolated by washing the parasites and by removing the internal structure with ultrasonic vibration or with pepsin digestion, the immunofluorescence persists (Baratawidjaja et al, 1963;Sulzer, 1965). Despite the other evidence, this finding seems to offer convincing evidence that the cuticle per se is antigenic: enzymes located in the cuticle may act as effective antigens (Sun, 1969 b).…”