“…Cuticular fluorescence indicating antigenantibody complexes has been reported in sections of schistosome adults (Andrade, Paronetto and Popper, 1961;Lichtenberg, 1964), Ascaris larvae (Crandall, Echevarria and Arean, 1963) and adult Fasciola (Thorpe, 1965) just as in the Clonorchis adults shown in the present study. When the cuticle of T. spiralis is isolated by washing the parasites and by removing the internal structure with ultrasonic vibration or with pepsin digestion, the immunofluorescence persists (Baratawidjaja et al, 1963;Sulzer, 1965). Despite the other evidence, this finding seems to offer convincing evidence that the cuticle per se is antigenic: enzymes located in the cuticle may act as effective antigens (Sun, 1969 b).…”