Fluorescent Analogs of Biomolecular Building Blocks: Design, Properties, and Applications

Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology

“…[1][2][3] The quest for insight into these ubiquitous biological processes, coupled with the limited range of naturally occurring fluorescent amino acids has led to development of versatile approaches for integrating fluorescent (or pro-fluorescent) motifs into peptides and proteins. 4,5 Currently, one of the most broadly applied methods for covalently labeling a protein with a fluorophore is to co-express the target with a naturally occurring fluorescent protein.…”

Density Functional Theoretical Study on the Acid Dissociation Constant of an Emissive Analogue of Guanine

“…Accordingly, the emissive analogues of nucleic acid bases have been the target of extensive studies because they can facilitate the fabrication of biophysical and discovery assays. [1][2][3] Recently, a fluorescent guanine analogue, 2-aminothieno [3,4-d]pyrimidine ( th G), was developed. 4 One of the essential criteria of a successful analogue is to minimize the structural and functional disturbances when replacing any native residue.…”

Preparation of fluorescent nucleic acids generating unique emission by primer extension reaction using pyrene-labeled deoxyuridine triphosphate derivatives

“…6,7 Certain kinds of deoxynucleotide triphosphate (dNTP) derivatives possessing a fluorescent reporter group at an appropriate position on the nucleobase can work as effective substrates for the enzymatic incorporation by polymerase and are used as fluorescent probes for the detection of the hybridization and amplification of DNA. 3,[8][9][10][11][12] Pyrene fluorophores with unique fluorescent properties, capable of generating an excited dimer with a characteristic green light, were conjugated to oligonucleotides for the structural study of the folding and development of single nucleotide polymorphisms (SNPs). In this study, we demonstrated the preparation of the deoxyuridine triphosphate derivatives labelled with the pyrene (Py) fluorophore through an ethylene glycol linker, and evaluation of the enzymatic incorporation of the triphosphate by DNA polymerase and the emission properties of the Py-incorporated DNA.…”

“…[1][2][3] To expand their applications as versatile tools, various kinds of oligodeoxynucleotide modified with functional molecules have been prepared by a general synthetic approach using the corresponding phosphoroamidite units during solid-phase DNA synthesis and the combination with post-synthetic oligonucleotides modification. 4,5 As an alternative approach to the conventional DNA synthesis for the preparation of the functionalized oligonucleotides, an enzymatic method using the polymerase extension (PEX) reaction has attracted much attention because of the facile preparation of longer oligonucleotides from DNA templates and multiple incorporation of functional groups/molecules into DNA based on the sequence of the templates.…”