Fluorescent Amplifying Recognition for DNA G-Quadruplex Folding with a Cationic Conjugated Polymer:  A Platform for Homogeneous Potassium Detection

He, Fang; Tang, Yanli; Wang, Shu; Li, Yuliang; Zhu, Daoben

doi:10.1021/ja051507i

Cited by 403 publications

(308 citation statements)

References 33 publications

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“…For Na + , Ca 2+ , Mg 2+ and NH4 + ions, the changes of relative excimer-emission intensities were very weak due to their weak binding affinity with thrombin-binding aptamer. 8,31 Hence, the proposed method represents good specificity for K + ions over Na + , Mg 2+ , Ca 2+ , and NH4 + ions at experimental condition. K + ions coexist with an excess of Na + ions in extracellular conditions.…”

Section: The Sensitivity and Specificity For Detecting Target K + Ionsmentioning

confidence: 87%

An Aptamer-based and Pyrene-labeled Fluorescent Biosensor for Homogeneous Detection of Potassium Ions

Huang

Zhao

2010

ANAL. SCI.

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Section: The Sensitivity and Specificity For Detecting Target K + Ionsmentioning

confidence: 87%

An Aptamer-based and Pyrene-labeled Fluorescent Biosensor for Homogeneous Detection of Potassium Ions

Huang

Zhao

2010

ANAL. SCI.

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“…12 The linear backbone of the CPs consists of a large number of chromic repeat units, so energy transfer in the whole backbone results in a big amplification of the fluorescence intensity. 13 Thus, they were always used as energy donors in fluorescence resonance energy transfer (FRET). [14][15][16] However, its application is restricted by some unfavorable factors, such as limited categories and complicated synthetic methods.…”

Section: Introductionmentioning

confidence: 99%

Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

Zheng

Huang

et al. 2009

ANAL. SCI.

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Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (DIRLS) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 mg mL -1 , and the detection limit (S/N = 3) was 17.0 ng mL -1 . The binding ratio and the binding constant between DNA and polymer were also provided.

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“…核酸适配体是一类 DNA 或 RNA 寡聚核苷酸, 能特异性识别金属离子 [3] 、有机小分子 [4] 、蛋白质 [5] 、病毒 [6] 、细菌 [7] , 甚至整个细胞 [8] . 核酸适配体在靶物质的诱导下可形成特殊的空间 [15,16] . 基于这一特点, 已发展了各种凝血酶检测的方法, 如荧光光谱法 [17,18] 、比色法 [19] 、表面等离子共振法 [20,21] 、电化学方法 [22,23] [30,31] .…”

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Highly Sensitive Protein Biosensor based on a Conjugated Polymer Brush

Liu¹,

Wang²,

Huang³

et al. 2016

Acta Chim. Sinica

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Simple and sensitive detection of proteins is crucial in biological analysis and medical diagnosis. Conjugated polymers (CPs) with π-conjugated backbones were recognized as having excellent light-harvesting capability and high fluorescent quantum yield. They have been widely used as an energy donor to amplify fluorescence signal via high efficient Föster resonance energy transfer (FRET). In particular, conjugated polymer brush with high charge density provides more possibilities due to stronger electrostatic interactions with negatively charged biomolecules. Here, we developed a highly sensitive protein biosensor for thrombin detection based on a conjugated polymer brush (PFNI) and a fluorescein-labeled aptamer (FAM-apt15). PFNI is a water-soluble cationic polyfluorene derivate with extremely high charge density (78 positive charges per repeat unit). PFNI can attract negatively charged aptamer through strong electrostatic interactions. In this case, the energy donor (PFNI) and acceptor (FAM) are in a close proximity, which results in an efficient FRET process and a high FRET signal. However, when the FAM-apt15 combines with the target protein, a rigid and big-sized G-quadruplex/thrombin complex formed. Due to the steric hindrance from the densely brush of PFNI, the distance between the two fluorophores increased significantly, leading to an inefficient FRET process and a low FRET signal. The strategy exhibits excellent specificity and the limit of detection (LOD) for thrombin in buffer was estimated to be 0.05 nmol/L. It also works well in diluted serum and a LOD of 0.2 nmol/L can be obtained. Compared to the biosensors based on traditional linear conjugated polymers, the sensitivity was improved by one order of magnitude. In addition, our strategy also shows the merits of simple, label-free, and low-cost because labeled DNA is much more expensive than unlabeled one. Based on the specific binding of aptamer and protein, this novel method can be extended to a highly sensitive detection of more proteins.

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Fluorescent Amplifying Recognition for DNA G-Quadruplex Folding with a Cationic Conjugated Polymer: A Platform for Homogeneous Potassium Detection

Cited by 403 publications

References 33 publications

An Aptamer-based and Pyrene-labeled Fluorescent Biosensor for Homogeneous Detection of Potassium Ions

An Aptamer-based and Pyrene-labeled Fluorescent Biosensor for Homogeneous Detection of Potassium Ions

Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

Highly Sensitive Protein Biosensor based on a Conjugated Polymer Brush

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