Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters

Luminescent Metal Nanoclusters for Potential Chemosensor Applications

“…The LODs of the Hg(II) sensors by both probes were estimated as 4 nM and 0.08 nM, respectively. Lee et al also presented DNA-based Ag clusters (Cyt12-Ag NCs; Φ = 43.9%) for detection of Ag(I) ions [93]. In this study, Cyt12-Ag NCs validated the recognition of Ag(I) ions through the shift of the fluorescence response from red to green with an LOD of 10 nM.…”

“…The linear ranges of Hg 2+ and Cu 2+ were from 0.01 μM to 0.5 μM and from 0 μM to 1.0 μM with corresponding LODs of 1.03 nM and 2.77 nM, respectively. This work also discriminated the detections in the Polyethyleneimine (PEI)-and GSH-capped Ag NCs have been extensively applied for detection of Cr(VI), Fe(III), and Pb(II) ions with affordable LODs [93][94][95][96]. However, GSH@Ag NCs for detection of Pb(II) ions were synthesized by a sonochemical method [96] rather that a wet chemical route.…”

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

“…Two 12 cytosine–containing mismatched DNA attached to AgNCs (Cyt12-AgNCs) undergo dimer formation in the presence of Ag + ions. The fluorescence of Cyt12-AgNCs changes from red to green on dimerization [ 101 •]. CA probe–based electrochemical nanosensor has been designed for Ag + detection in antimicrobial drug, sulfadiazine.…”

Pre‐Incubation of Auric Acid with DNA Is Unnecessary for the Formation of DNA‐Templated Gold Nanoclusters

“…[2,3] Among the stabilizing reagents, [5][6][7][8][9][10][11][12][13][14][15][16][17][18] DNA is an excellent type of template because (1) the emission spectrum can be conveniently encoded by sequencea nd conformationalc hanges of the DNA scaffold, (2) the formed photoluminescent DNA-templated silver nanoclusters (DNA-Ag NCs) can be easily integrated into nano-assembliesv ia hybridization, and (3) the functionalizationo ft he photoluminophore can be easily rendered to facilitate the bio-recognition via extended sequences. [19] Compared to Ag NCs, Au NCs have better chemical stability,p hotostability,a nd longer fluorescencel ifetime, and thus shouldf ind widea pplications with the advance of syntheses.To date, DNA-templatedA gN Cs find ab readtho fa pplications, including cellular imaging [20][21][22] and detection of various analytes including metal ions, [23][24][25] smallm olecules, [26][27][28][29] proteins, [30][31][32] miRNA [33][34][35] and DNA. [36,37] For Au NCs, dendrimers, [38,39] thiols, [40][41][42][43] peptides, [44] and proteins…”

“…To date, DNA-templatedA gN Cs find ab readtho fa pplications, including cellular imaging [20][21][22] and detection of various analytes including metal ions, [23][24][25] smallm olecules, [26][27][28][29] proteins, [30][31][32] miRNA [33][34][35] and DNA. [36,37] For Au NCs, dendrimers, [38,39] thiols, [40][41][42][43] peptides, [44] and proteins [45][46][47] have already been used as templates.H owever,l ittle research has been reported on DNA-templated Au NCs, and applications still remain undiscovered.…”