Fluorescence Studies into the Effect of Plasmonic Interactions on Protein Function

Nieder, Jana B.; Bittl, Robert; Brecht, Marc

doi:10.1002/anie.201002172

Cited by 63 publications

(75 citation statements)

References 42 publications

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“…As the fluorophore spends less time in the excited state, the photostability will increase and thus a higher number of photons can be detected before irreversible photobleaching occurs. 27,28 To date only a few studies on emission enhancement of lightharvesting complexes have been reported using chemically synthesized metal nanostructures such as silver island films, 29,30 gold, [31][32][33] and silver nanoparticles. 34 In most studies the individual contributions of excitation and QY changes to the total fluorescence enhancement have not been disentangled.…”

Section: Introductionmentioning

confidence: 99%

Nanoantenna enhanced emission of light-harvesting complex 2: the role of resonance, polarization, and radiative and non-radiative rates

Wientjes

Renger

Curto

et al. 2014

Phys. Chem. Chem. Phys.

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Nanoantennae show potential for photosynthesis research for two reasons; first by spatially confining light for experiments which require high spatial resolution, and second by enhancing the photon emission of single light-harvesting complexes. For effective use of nanoantennae a detailed understanding of the interaction between the nanoantenna and the light-harvesting complex is required.Here we report how the excitation and emission of multiple purple bacterial LH2s (light-harvesting complex 2) are controlled by single gold nanorod antennae. LH2 complexes were chemically attached to such antennae, and the antenna length was systematically varied to tune the resonance with respect to the LH2 absorption and emission. There are three main findings. (i) The polarization of the LH2 emission is fully controlled by the resonant nanoantenna. (ii) The largest fluorescence enhancement, of 23 times, is reached for excitation with light at l = 850 nm, polarized along the long antenna-axis of the resonant antenna. The excitation enhancement is found to be 6 times, while the emission efficiency is increased 3.6 times. (iii) The fluorescence lifetime of LH2 depends strongly on the antenna length, with shortest lifetimes of B40 ps for the resonant antenna. The lifetime shortening arises from an 11 times resonant enhancement of the radiative rate, together with a 2-3 times increase of the non-radiative rate, compared to the off-resonant antenna. The observed length dependence of radiative and non-radiative rate enhancement is in good agreement with simulations. Overall this work gives a complete picture of how the excitation and emission of multi-pigment light-harvesting complexes are influenced by a dipole nanoantenna.

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Section: Introductionmentioning

confidence: 99%

Nanoantenna enhanced emission of light-harvesting complex 2: the role of resonance, polarization, and radiative and non-radiative rates

Wientjes

Renger

Curto

et al. 2014

Phys. Chem. Chem. Phys.

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“…While molecular adsorbates on the surfaces of nanoparticles can modify the surface plasmon resonances [4], the plasmonic interaction can also aect protein function [5]. As a consequence, a deep understanding of the fundamental interaction mechanisms between metal nanostructures and proteins is necessary for the design of hybrids with tailored…”

Section: Introductionmentioning

confidence: 99%

Interactions of Photosystem I with Plasmonic nanostructures

Hussels

Nieder

2012

Acta Phys. Pol. A

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The plasmonic interaction eects of various nanostructures on the uorescence properties of photosystem I as found by single-molecule spectroscopy are summarized. The used nanostructures are spherical Au nanoparticles, silver island lms as well as hexagonal arrays of nanometer-sized Au-and Ag-triangles (the Fischer patterns). The uorescence emission of photosystem I is intensied due to coupling with these nanostructures. For single photosystem I complexes, enhancement factors of up to 37 were observed. The average enhancements vary between 2.2 for Au Fischer pattern and 9 for spherical Au nanoparticles. The enhancement of the uorescence of photosystem I demonstrates in all cases a strong wavelength dependence. This wavelength dependence can be explained by the spatially largely extended multichromophore composition of photosystem I complexes. From the viewpoint of the usability of these nanostructures for spectroscopic signal enhancement, the Fischer patterns are benecial, due to their very low autoluminescence.

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“…While the interactions between plasmons and simple nanostructures such as organic dyes or semiconductor nanocrystals is relatively well described and understood, application of metallic nanoparticles to multipigment structures has started just recently (Carmeli, 2010;Govorov, 2008;Kim, 2011;Nieder, 2010). Light-harvesting complexes, or more generally, photosynthetic complexes, are quite appealing in this regard as they not only provide an interesting biomolecular system for studying plasmon effect on both the optical properties of pigments and the energy transfer between them, but also they could offer attractive potential application route in photovoltaics (Atwater & Polman, 2010;Mackowski, 2010).…”

Section: Introductionmentioning

confidence: 99%

Metallic Nanoparticles Coupled with Photosynthetic Complexes

Maćkowski¹

2012

Smart Nanoparticles Technology

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Fluorescence Studies into the Effect of Plasmonic Interactions on Protein Function

Cited by 63 publications

References 42 publications

Nanoantenna enhanced emission of light-harvesting complex 2: the role of resonance, polarization, and radiative and non-radiative rates

Nanoantenna enhanced emission of light-harvesting complex 2: the role of resonance, polarization, and radiative and non-radiative rates

Interactions of Photosystem I with Plasmonic nanostructures

Metallic Nanoparticles Coupled with Photosynthetic Complexes

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