Fluorescence Spectral Properties of 2,5-Diphenyl-1,3,4-oxadiazole with Two-Color Two-Photon Excitation

Abstract

We observed fluorescence emission from 2,5-diphenyl-1,3,4-oxadiazole (PPD) resulting from two-photon excitation with two different wavelengths near 380 and 760 nm. For this two-color two-photon (2C2P) excitation the emission spectra and intensity decays were the same as observed with single-photon excitation with an equivalent energy at 250 nm. The two-color two-photon-induced emission was observed when the PPD sample was illuminated with both wavelengths, but only when the picosecond laser pulses were spatially and temporally overlapped. The signal was about 70-fold and 1000-fold less for illumination at 380 or 760 nm alone, respectively. When illuminated with both wavelengths, the emission intensity of PPD depended quadratically on the total illumination power, when both beams were simultaneously attenuated to the same extent, indicating two-photon excitation. When the intensity at one wavelength was attenuated, the signal depended linearly on the power at each wavelength, indicating the participation of one-photon at each wavelength to the excitation process. For 2C2P excitation the time-zero anisotropy was larger than possible for single-photon excitation and was consistent with collinear electronic transitions for both wavelengths. The intensity depended on the polarization of each beam in a manner consistent with collinear transitions. These results demonstrate that two-color two-photon excitation can be readily observed with modern laser sources. This phenomenon can have numerous applications in the chemical and biomedical sciences, as a method for spatial localization of the measured volume.

Energy transfer and spectroscopic characterization of a perylenetetracarboxylic diimide (PDI) hexamer

“…The EADS represents the spectral evolution with successively increasing lifetime. The time-resolved anisotropic decay r(t) was calculated from the decay curves under the parallel polarizations I || (t) and perpendicular polarizations I ⊥ (t) based on the tail matching correction according to equation (3): [23][24][25] …”

Fluorescence Anisotropy Controlled by Light Quenching

“…Precise adjustment of time delay was obtained with two-color two-photon fluorescence. This phenomenon was recently studied in this laboratory (12,16,17). A solution of p-terphenyl in cyclohexane was simultaneously illuminated with 370 nm and 740 nm pulses.…”

The polarisation of two-photon excited fluorescence in rotating molecules

“…), see [7,8,[11][12][13][14][15][16] and references therein, however a number of studies have been carried out in the gas phase [17][18][19][20][21][22][23][24][25]. The gas phase/molecular beam studies are of fundamental importance for understanding the TPEF dynamics of polyatomic molecules in the collision-free regime because they can be supported by high accuracy quantum mechanical ab initio calculations which in most cases are not available for the condensed matter studies.…”