Fluorescence spectra of atmospheric aerosol particles measured using one or two excitation wavelengths: Comparison of classification schemes employing different emission and scattering results

Pan, Yong‐Le; Hill, Steven C.; Pinnick, Ronald G.; Huang, Hermes; Bottiger, Jerold R.; Chang, Richard K.

doi:10.1364/oe.18.012436

Cited by 78 publications

(57 citation statements)

References 45 publications

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“…As judged by WIBS, the multi-channel fluorescence response observed from the seven Penicillium species aerosolized here are in general agreement with those of the Penicillium notatum by Toprak and Schnaiter (2013); they reported fluorescence emissions from P. notatum commensurate with those observed here (dominance of type A, with ascendance of type C), where these aerosolized microbes were mixed with ammonium sulfate aerosol in smallchamber studies. Pan et al (2010) also reported fluorescence (type B) associated with aerosolized B. subtilis spores similar to that reported here. The spectral distributions of B. subtilis spores reported here, and by Pan et al (2010), suggest that these aerosolized spores had markedly different fluorescence than that observed from vegetative bacterial cells with nearly the same EOD.…”

Section: Composite Optical Recognition Patterns Of Bioaerosol Classessupporting

confidence: 86%

“…While many PBAPs fluoresce, interpreting UV-LIF measurements for bioaerosol characterization presents significant interpretive challenges, some of which have been successfully addressed using cluster analyses (Pinnick et al, 2013;Gabey et al, 2013;Crawford et al, 2015;Pan et al, 2015). In addition to classic Bayesian clustering approaches, referential methods can also be applied to analyze fluorescence distributions with selected optical properties, which may in turn be leveraged for environmental comparison(s).…”

Section: Introductionmentioning

confidence: 99%

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Chamber catalogues of optical and fluorescent signatures distinguish bioaerosol classes

et al. 2016

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Abstract. Rapid bioaerosol characterization has immediate applications in the military, environmental and public health sectors. Recent technological advances have facilitated single-particle detection of fluorescent aerosol in near real time; this leverages controlled ultraviolet exposures with single or multiple wavelengths, followed by the characterization of associated fluorescence. This type of ultraviolet induced fluorescence has been used to detect airborne microorganisms and their fragments in laboratory studies, and it has been extended to field studies that implicate bioaerosol to compose a substantial fraction of supermicron atmospheric particles. To enhance the information yield that newgeneration fluorescence instruments can provide, we report the compilation of a referential aerobiological catalogue including more than 50 pure cultures of common airborne bacteria, fungi and pollens, recovered at water activity equilibrium in a mesoscale chamber (1 m 3 ). This catalogue juxtaposes intrinsic optical properties and select bandwidths of fluorescence emissions, which manifest to clearly distinguish between major classes of airborne microbes and pollens.

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Section: Composite Optical Recognition Patterns Of Bioaerosol Classessupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Chamber catalogues of optical and fluorescent signatures distinguish bioaerosol classes

et al. 2016

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“…As a result, a number of real-time and commercial instruments including the Ultraviolet Aerodynamic Particle Sizer (UV-APS; TSI Inc., Shoreview, MN, USA) and the Waveband Integrated Bioaerosol Sensor (WIBS; Droplet Measurement Technologies, Longmont, CO, USA) are being commonly used in bioaerosol research communities (e.g., Agranovski et al, 2003;Bhangar et al, 2014;Brosseau et al, 2000;Foot et al, 2008;Huffman et al, 2010;Perring et al, 2015;Stanley et al, 2011;Toprak and Schnaiter, 2013). The main principle common to these techniques is the detection of intrinsic fluorescence from fluorophores such as amino acids, coenzymes, vitamins, and pigments that ubiquitously occur in aerosols of biological origin (e.g., Hill et al, 2009;Pan et al, 2010;Pöhlker et al, 2012Pöhlker et al, , 2013. These PBAP represent a diverse and dynamic subset of airborne particles, consisting of whole organisms like bacteria, viruses, archaea, algae, fungi, and related reproductive units (e.g., pollen, bacterial and fungal spores), as well as decaying biomass and fragments from plants or insects (e.g., Deepak and Vali, 1991;Després et al, 2012;Jaenicke, 2005;Madelin, 1994;Pöschl, 2005).…”

Section: Introductionmentioning

confidence: 99%

Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods

et al. 2018

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Abstract. Fluorescent dyed polystyrene latex spheres (PSLs) are commonly used for characterization and calibration of instruments detecting fluorescence signals from particles suspended in the air and other fluids. Instruments like the Ultraviolet Aerodynamic Particle Sizer (UV-APS) and the Waveband Integrated Bioaerosol Sensor (WIBS) are widely used for bioaerosol research, but these instruments present significant technical and physical challenges requiring careful characterization with standard particles. Many other research communities use flow cytometry and other instruments that interrogate fluorescence from individual particles, and these also frequently rely on fluorescent PSLs as standards. Nevertheless, information about physical properties of commercially available PSLs provided by each manufacturer is generally proprietary and rarely available, making their use in fluorescence validation and calibration very difficult.This technical note presents an overview of steady-state fluorescence properties of fluorescent and non-fluorescent PSLs, as well as of polystyrene-divinylbenzene (PS-DVB) particles, by using on-and offline spectroscopic techniques. We show that the "fluorescence landscape" of PSLs is more complex than the information typically provided by manufacturers may imply, especially revealing multimodal emission patterns. Furthermore, non-fluorescent PSLs also exhibit defined patterns of fluorescent emission originating from a mixture of polystyrene and detergents, which becomes a crucial point for fluorescence threshold calibrations and qualitative comparison between instruments. By comparing PSLs of different sizes, but doped with the same dye, changes in emission spectra from bulk solutions are not immediately obvious. On a single-particle scale, however, fluorescence intensity values increase with increasing particle size. No significant effect in the fluorescence signatures was detectable by comparing PSLs in dry vs. wet states, indicating that solvent water may only play a minor role as a fluorescence quencher.Because information provided by manufacturers of commercially available PSLs is generally very limited, we provide the steady-state excitation-emission matrices (EEMs) of PSLs as open-access data within the Supplement. Detergent and solvent effects are also discussed in order to provide information not available elsewhere to researchers in the bioaerosol and other research communities. These data are not meant to serve as a fundamental library of PSL properties because of the variability of fluorescent properties between batches and as a function of particle aging and agglomeration. The data presented, however, provide a summary of spectral features which are consistent across these widely used fluorescent standards. Using these concepts, further checks will likely be required by individual researchers using specific lots of standards.

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“…Some of these instruments measure single particle elastic scattering, and/or single particle fluorescence, and/or laser-or spark-induced breakdown spectroscopy (LIBS/SIBS) and some are commercially available [3]. Laser induced fluorescence (LIF), especially dual-wavelength UV-LIF has been demonstrated for near-real time detection and partial classification of bioaerosols particles [4][5][6][7][8][9]. The technique was shown to be capable of differentiating pollens from various plant species [9,10].…”

Section: Introductionmentioning

confidence: 99%

Photophoretic trapping-Raman spectroscopy for single pollens and fungal spores trapped in air

Wang

Pan

Hill

et al. 2015

Journal of Quantitative Spectroscopy and Radiative Transfer

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a b s t r a c tPhotophoretic trapping-Raman spectroscopy (PTRS) is a new technique for measuring Raman spectra of particles that are held in air using photophoretic forces. It was initially demonstrated with Raman spectra of strongly-absorbing carbon nanoparticles (Pan et al.[44] (Opt Express 2012)). In the present paper we report the first demonstration of the use of PTRS to measure Raman spectra of absorbing and weakly-absorbing bioaerosol particles (pollens and spores). Raman spectra of three pollens and one smut spore in a size range of 6.2-41.8 mm illuminated at 488 nm are shown. Quality spectra were obtained in the Raman shift range of 1600-3400 cm À 1 in this exploratory study. Distinguishable Raman scattering signals with one or a few clear Raman peaks for all four aerosol particles were observed within the wavenumber region 2940-3030 cm À 1 . Peaks in this region are consistent with previous reports of Raman peaks in the 1600-3400 cm À 1 range for pollens and spores excited at 514 nm measured by a conventional Raman spectrometer. Noise in the spectra, the fluorescence background, and the weak Raman signals in most of the 1600-3400 cm À 1 region make some of the spectral features barely discernable or not discernable for these bioaerosols except the strong signal within 2940-3030 cm À 1 . Up to five bands are identified in the three pollens and only two bands appear in the fungal spore, but this may be because the fungal spore is so much smaller than any of the pollens. The fungal spore signal relative to the air-nitrogen Raman band is approximately 10 times smaller than that ratio for the pollens. The five bands are tentatively assigned to the CH 2 symmetric stretch at 2948 cm À 1 , CH 2 Fermi resonance stretch at 2970 cm À 1 , CH 3 symmetric stretch at 2990 cm À 1 , CH 3 out-of-plane end asymmetric stretch at 3010 cm À 1 , and unsaturated ¼ CH stretch at 3028 cm À 1 . The two dominant bands of the up-to-five Raman bands in the 2940-3030 cm À 1 region have a consistent band spacing of 25 cm À 1 in all four aerosols. Finally we discuss improvements to the PTRS that should provide a system which can trap a higher fraction of particle types and obtain Raman spectra over a larger range (e.g., 200-3600 cm À 1 ) than those achieved here.Published by Elsevier Ltd.

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Fluorescence spectra of atmospheric aerosol particles measured using one or two excitation wavelengths: Comparison of classification schemes employing different emission and scattering results

Cited by 78 publications

References 45 publications

Chamber catalogues of optical and fluorescent signatures distinguish bioaerosol classes

Chamber catalogues of optical and fluorescent signatures distinguish bioaerosol classes

Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods

Photophoretic trapping-Raman spectroscopy for single pollens and fungal spores trapped in air

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