Fluorescence Resonance Energy Transfer of Monomer via Photoisomerization

Abstract

We experimentally report a new photoisomerization mechanism of fluorescence resonance energy transfer (FRET) of monomer via photoisomerization. Firstly, a new photoisomerization was firstly report by us, in which trans, trans-1,4-Diphenyl-1,3-butadiene (tt-DPB) is photoisomerized to D-DPB (D stands for rotating double bond) by rotating double bond, evidenced by the three dimensional (3D) and 2D excitationemission mappings, and firmly supported by theoretical calculations on potential energy surfaces. Secondly, absorption energy of the photoisomerized D-DPB is resonated with the fluorescence energy of tt-DPB. By the electronic state excitation of tt-DPB, the fluorescence of D-DPB is observed, which provide evidence of the FRET of DPB via photoisomerization. Our results provide a new mechanism on FRET of monomer via photoisomerization.

Plasmon‐Enhanced Fluorescence Resonance Energy Transfer

“…Concluded that the kinetics of photoisomerization tt‐DPB to D‐DPB are researched and shown in Figure (a) . It was reported that they have two twisted processes in the ct‐DTB isomer, but here, expected that they are tt‐DPB‐>ct‐DPB‐>cc‐DPB in the ground state (see Figure (b)) . Note that the path of photoisomeriaztion can be seen from Figure (d), and the potential barrier of tt‐DPB‐>cc‐DPB (0.128 u) is significant larger than that of tt‐DPB‐>D‐DPB (0.016 au).…”

“…also designed the potential energy surface by optimizing the relative dihedral angle of all ground and excited state. Concluded that the kinetics of photoisomerization tt‐DPB to D‐DPB are researched and shown in Figure (a) . It was reported that they have two twisted processes in the ct‐DTB isomer, but here, expected that they are tt‐DPB‐>ct‐DPB‐>cc‐DPB in the ground state (see Figure (b)) .…”

“…have studied the FRET of monomer via photoisomerization for trans, trans‐1,4‐Diphenyl‐1,3‐butadiene (tt‐DPB)>D‐DPB. Note that there is the resonant phenomenon between the D‐DPB's excitation energy level and the tt‐DPB's fluorescence energy level, and the energy transferred from tt‐DPB's absorption energy to that of D‐DPB through the fluorescence of resonant, and then fluorescence of D‐DPB are observed at 450 nm (see Figure ) . Therefore, the FRET indeed occur for DPB monomer via photoisomerization.…”

“…Therefore, the FRET indeed occur for DPB monomer via photoisomerization. They also measured the 3D and 2D spectra of emission (see Figure ), and concluded that presenting a strong “dark” absorption near 375 nm and the strongest emission is around 450 nm by the comparison of Figure (a) and Figure …”

“… The Mapping of Excitation‐Emission spectra of 1′4‐dipheylbutadiene, where (a) and (b) are 3D and 2D spectra, respectively …”