Fluorescence resonance energy transfer between bovine serum albumin and fluoresceinamine

Abstract

Physical binding-mediated organic dye direct-labelling of proteins could be a promising technology for bio-nanomedical applications. Upon binding, it was found that fluorescence resonance energy transfer (FRET) occurred between donor bovine serum albumin (BSA; an amphiphilic protein) and acceptor fluoresceinamine (FA; a hydrophobic fluorophore), which could explain fluorescence quenching found for BSA. FRET efficiency and the distance between FA and BSA tryptophan residues were determined to 17% and 2.29 nm, respectively. Using a spectroscopic superimposition method, the saturated number of FAs that bound to BSA was determined as eight to give a complex formula of FA8-BSA. Finally, molecular docking between BSA and FA was conducted, and conformational change that occurred in BSA upon binding to FA molecules was also studied by three-dimensional fluorescence microscopy.

Interaction and energy transfer studies between bovine serum albumin and CdTe quantum dots conjugates: CdTe QDs as energy acceptor probes

“…A slight blue shift of 1 nm in the fluorescence peak of BSA in presence of QDs as compared to the spectrum of BSA alone, is observed as the concentration of CdTe QDs gradually increases. This variation in the characteristic fluorescence peak of BSA indicates the interaction occurring between BSA and CdTe QDs …”

“…Within the family of serum albumins, BSA is used as a model in studying protein‐ligand binding and has 76% sequence homology with human serum albumin (HSA) . BSA has three intrinsic fluorophores–tryptophan (Trp), tyrosine (Tyr) and phenylalanine (Phe).…”

Unexpected Reactivity of N‐Acyl‐Benzotriazoles with Aromatic Amines in Acidic Medium (ABAA Reaction)

“…We then decided to exemplify this reaction to other aromatic amines, including a few that are included in compounds of biological interest. We have studied the reactivity of Boc‐Gly‐Bt 11 with both 7‐amino‐4‐methylcoumarin (AMC) 12 , and fluoresceinamine 13 , which are used for the synthesis of fluorescent probes (Scheme ) , . These syntheses are often quite complicate and generally led to the expected compounds in low yields , …”

Interaction study on bovine serum albumin physically binding to silver nanoparticles: Evolution from discrete conjugates to protein coronas

“…The hydrogen bonds can be formed between the dense carboxylic groups on the backbone of the AP and most of amino groups on the surface of BSA proteins. For studying the conformational information of proteins, three dimensional fluorescence spectroscopy (3DFS) is a facile and powerful method to provide more detailed information about the conformational and micro-environmental changes of protein that combined with molecules [35,42].…”