Fluorescence resonance energy transfer: A promising tool for investigation of the interaction between 1-anthracene sulphonate and serum albumins

Banerjee, P.; Ghosh, Saptaparni; Sarkar, Arindam; Bhattacharya, Sumanta

doi:10.1016/j.jlumin.2010.10.022

Cited by 79 publications

(32 citation statements)

References 23 publications

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“…The binding constant K b and the number of binding sites per albumin molecule can be determined according to the Scatchard Eq. (4): [80]. The values of K q and K bin for the ligand and complex suggested that the Cu complex interacts with BSA more strongly than the ligand.…”

Section: Binding Constant and Number Of Binding Sites And Gibbs Free mentioning

confidence: 96%

A novel Schiff base derived from the gabapentin drug and copper (II) complex: Synthesis, characterization, interaction with DNA/protein and cytotoxic activity

Shokohi-pour

Chiniforoshan

Momtazi‐Borojeni

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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A novel Schiff base [C20H23NO3], has been prepared and characterized using FT-IR, UV-vis, (1)H NMR spectroscopy, elemental analysis and X-ray crystallography. A copper (II) complex [Cu(C20H22NO3)2]·H2O has also been synthesized and characterized. The new ligand and complex thus obtained were investigated by their interaction with calf thymus DNA and BSA using electronic absorption spectroscopy, fluorescence spectroscopy, and thermal denaturation. The intrinsic binding constants Kb of the ligand and Cu (II) complex, with CT-DNA obtained from UV-vis absorption studies were 1.53×10(4)M(-1) and 3.71×10(5)M(-1), respectively. Moreover the addition of the two compounds to CT-DNA (1:2) led to an increase of the melting temperature of DNA up to around 2.61°C for the ligand and 3.99°C for the Cu (II) complex. The ligand and Cu (II) complex bind to CT-DNA via a partial intercalative, as shown by the experimental data. In addition, the albumin interactions of the two compounds were studied by fluorescence quenching spectra, the results indicating that the binding mechanism is a static quenching process. The in vitro cytotoxicity of the two compounds on three different cancer cell lines was evaluated by MTT assay. The results showed that the copper complex exerted enhanced cytotoxicity compared with the Schiff base ligand; thereby, this complex clearly implies a positive synergistic effect. Furthermore, the copper complex showed a high, selective, and dose-dependent cytotoxicity against cancer cell lines.

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Section: Binding Constant and Number Of Binding Sites And Gibbs Free mentioning

confidence: 96%

A novel Schiff base derived from the gabapentin drug and copper (II) complex: Synthesis, characterization, interaction with DNA/protein and cytotoxic activity

Shokohi-pour

Chiniforoshan

Momtazi‐Borojeni

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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show abstract

“…When small molecules bind independently to a set of equivalent sites on a macromolecule, the equilibrium between the free and bound molecules is given by the equation [64,65]:…”

Section: Binding Constant and Number Of Binding Sites And Gibbs Free mentioning

confidence: 99%

Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group

Karami

Shirani-Sarmazeh

Hosseini-Kharat

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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“…For static quenching, the binding constant (K) and the number of binding sites (n) are given by the following equation [57,58]:…”

Section: Binding Constant and Number Of Binding Sitesmentioning

confidence: 99%

Synthesis and structure of dicopper(II) complexes bridged by N-(5-chloro-2-hydroxyphenyl)-N′-[3-(methy lamino)propyl]oxamide: Evaluation of DNA/protein binding, DNA cleavage, and in vitro anticancer activity

Zhu

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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Fluorescence resonance energy transfer: A promising tool for investigation of the interaction between 1-anthracene sulphonate and serum albumins

Cited by 79 publications

References 23 publications

A novel Schiff base derived from the gabapentin drug and copper (II) complex: Synthesis, characterization, interaction with DNA/protein and cytotoxic activity

A novel Schiff base derived from the gabapentin drug and copper (II) complex: Synthesis, characterization, interaction with DNA/protein and cytotoxic activity

Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group

Synthesis and structure of dicopper(II) complexes bridged by N-(5-chloro-2-hydroxyphenyl)-N′-[3-(methy lamino)propyl]oxamide: Evaluation of DNA/protein binding, DNA cleavage, and in vitro anticancer activity

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