Fluorescence recovery after photobleaching reveals high cycling dynamics of plasma membrane aquaporins in Arabidopsis roots under salt stress

Luu, Doan‐Trung; Martinière, Alexandre; Sorieul, Mathias; Runions, John; Maurel, Christophe

doi:10.1111/j.1365-313x.2011.04841.x

Cited by 116 publications

(117 citation statements)

References 41 publications

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“…Salt stress is known to cause a dramatic increase in endocytic uptake (Leshem et al, 2007;Luu et al, 2012), resulting in the generation of intravesicular reactive oxygen species that act as second messengers in salt stress-elicited signaling pathways (Leshem et al, 2007;Golani et al, 2013). Salt stress is reported to elicit enhanced uptake and recycling of PM aquaporins in Arabidopsis root epidermis (Li et al, 2011;Luu et al, 2012). Such enhanced uptake is postulated to be partially due to the induction of a sterol-sensitive (lipid raft-dependent) pathway, which is not responsible for the uptake of aquaporins under control conditions.…”

Section: Discussionmentioning

confidence: 99%

Salt-Induced Remodeling of Spatially Restricted Clathrin-Independent Endocytic Pathways in Arabidopsis Root

et al. 2015

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Endocytosis is a ubiquitous cellular process that is characterized well in animal cells in culture but poorly across intact, functioning tissue. Here, we analyze endocytosis throughout the Arabidopsis thaliana root using three classes of probes: a lipophilic dye, tagged transmembrane proteins, and a lipid-anchored protein. We observe a stratified distribution of endocytic processes. A clathrin-dependent endocytic pathway that internalizes transmembrane proteins functions in all cell layers, while a sterol-dependent, clathrin-independent pathway that takes up lipid and lipid-anchored proteins but not transmembrane proteins is restricted to the epidermal layer. Saline stress induces a third pathway that is clathrinindependent, nondiscriminatory in its choice of cargo, and operates across all layers of the root. Concomitantly, small acidic compartments in inner cell layers expand to form larger vacuole-like structures. Plants lacking function of the Rab-GEF (guanine nucleotide exchange factor) VPS9a (vacuolar protein sorting 9A) neither induce the third endocytic pathway nor expand the vacuolar system in response to salt stress. The plants are also hypersensitive to salt. Thus, saline stress reconfigures clathrin-independent endocytosis and remodels endomembrane systems, forming large vacuoles in the inner cell layers, both processes correlated by the requirement of VPS9a activity.

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Section: Discussionmentioning

confidence: 99%

Salt-Induced Remodeling of Spatially Restricted Clathrin-Independent Endocytic Pathways in Arabidopsis Root

et al. 2015

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“…6). A role for the endocytic pathway in DIM-enriched protein localization at specific sites in root cells and seedlings was accordingly demonstrated for aquaporin (Luu et al, 2012), dynaminrelated protein (Konopka et al, 2008), and flotillin (Li et al, 2012).…”

Section: Cryptogein Transiently Induces a Modification In Tobacco Celmentioning

confidence: 99%

Modification of Plasma Membrane Organization in Tobacco Cells Elicited by Cryptogein

et al. 2013

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Lipid mixtures within artificial membranes undergo a separation into liquid-disordered and liquid-ordered phases. However, the existence of this segregation into microscopic liquid-ordered phases has been difficult to prove in living cells, and the precise organization of the plasma membrane into such phases has not been elucidated in plant cells. We developed a multispectral confocal microscopy approach to generate ratiometric images of the plasma membrane surface of Bright Yellow 2 tobacco (Nicotiana tabacum) suspension cells labeled with an environment sensitive fluorescent probe. This allowed the in vivo characterization of the global level of order of this membrane, by which we could demonstrate that an increase in its proportion of ordered phases transiently occurred in the early steps of the signaling triggered by cryptogein and flagellin, two elicitors of plant defense reactions. The use of fluorescence recovery after photobleaching revealed an increase in plasma membrane fluidity induced by cryptogein, but not by flagellin. Moreover, we characterized the spatial distribution of liquid-ordered phases on the membrane of living plant cells and monitored their variations induced by cryptogein elicitation. We analyze these results in the context of plant defense signaling, discuss their meaning within the framework of the "membrane raft" hypothesis, and propose a new mechanism of signaling platform formation in response to elicitor treatment.

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“…The decline in the associated proteins does not, however, simply reflect degradation, but re-localisation to intracellular structures (Boursiac et al 2005). Studies of PIP1;2 and PIP2;1, in particular, have found that dispersion is heterogeneous and that salinity stress increases their internalisation and reduces their presence in the plasma membrane (Li et al 2011;Luu et al 2012). The subcellular trafficking is mediated by both clathrin and membrane raft pathways.…”

Section: Water and Transpirationmentioning

confidence: 99%

The integration of activity in saline environments: problems and perspectives

Cheeseman

2013

Functional Plant Biol.

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Abstract. The successful integration of activity in saline environments requires flexibility of responses at all levels, from genes to life cycles. Because plants are complex systems, there is no 'best' or 'optimal' solution and with respect to salt, glycophytes and halophytes are only the ends of a continuum of responses and possibilities. In this review, I briefly examine seven major aspects of plant function and their responses to salinity including transporters, secondary stresses, carbon acquisition and allocation, water and transpiration, growth and development, reproduction, and cytosolic function and 'integrity'. I conclude that new approaches are needed to move towards understanding either organismal integration or 'salt tolerance', especially cessation of protocols dependent on sudden, often lethal, shock treatments and the embracing of systems level resources. Some of the tools needed to understand the integration of activity and even 'salt stress' are already in hand, such as those for whole-transcriptome analysis. Others, ranging from discovery studies of the nature of the cytosol to expanded tool kits for proteomic, metabolomic and epigenomic studies, still need to be further developed. After resurrecting the distinction between applied stress and the resultant strain and noting that with respect to salinity, the strain is manifest in changes at all -omic levels, I conclude that it should be possible to model and quantify stress responses.

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Fluorescence recovery after photobleaching reveals high cycling dynamics of plasma membrane aquaporins in Arabidopsis roots under salt stress

Cited by 116 publications

References 41 publications

Salt-Induced Remodeling of Spatially Restricted Clathrin-Independent Endocytic Pathways in Arabidopsis Root

Salt-Induced Remodeling of Spatially Restricted Clathrin-Independent Endocytic Pathways in Arabidopsis Root

Modification of Plasma Membrane Organization in Tobacco Cells Elicited by Cryptogein

The integration of activity in saline environments: problems and perspectives

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