Fluorescence quenching of bioactive molecules by nanodiamonds

Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions

“…As was shown by us in ref , nanodiamonds actively quench the fluorescence of lysozymes through the static quenching mechanism. The effective quenching of lysozyme fluorescence by nanodiamonds improves the DNDs’ visualization in biotissues as well as provides a possible way to track the loading and subsequent unloading of drugs from the DND surface.…”

“…The maximum of the absorbance spectra of lysozyme in water is about 280 nm. The fluorescence spectrum of lysozyme is a broad band with a maximum around 340 nm . When a DND + lysozyme complex is excited by radiation at a wavelength of 280 nm, mainly only the lysozyme fluoresces (and DND actively scatters light) and when excited at a 405 nm wavelength, the lysozyme does not fluoresce: all fluorescence of the complex is due to DNDs.…”

“…Lysozymes also possess fluorescence. To the best of the authors’ knowledge, only in their previous work was the question of the effect of the interaction of nanodiamonds with lysozymes on the fluorescence of lysozymes raised. The results of that work are consistent with those on the effect on lysozyme fluorescence of other nanoparticles, Fe 3 O 4 –polymer nanocomposites, and gold nanoparticles, during the formation of the complexes through physical adsorption: the interaction of lysozymes with adsorbing nanoparticles in a suspension leads to the quenching of protein fluorescence.…”