Fluorescence properties of Phycocyanin and Phycocyanin-human serum albumin complex

Seyedi, Solaleh; Parvin, Parviz; Jafargholi, Amir; Jelvani, S.; Shahabi, Majid; Shahbazi, Maryam; Mohammadimatin, Parisa; Moafi, A.

doi:10.1016/j.saa.2020.118468

Cited by 34 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the fluorescence intensity decreases obviously after the addition of the Fe complex, and due to that, the complex replaces EB in the EB-DNA system ( Figure 1 ). The binding constant ( K b ) and the number of average binding sites (n) are calculated according to the Stern–Volmer Eqs 1 , 2 ( Seyedi et al, 2020 ; Shao et al, 2021 ).

…”

Section: Resultsmentioning

confidence: 99%

“…The K b is 5.00–5.96 × 10 5 M −1 , and the average binding-site number is about 1.2. The positive value of ∆S (and ∆H near to 0) suggested that electrostatic interactions exist between the complex and CT-DNA ( Yang et al, 2011 ; Heydari and Mansouri-Torshizi, 2016 ; Seyedi et al, 2020 ; Shao et al, 2021 ). The negative value of ∆G shows the spontaneity of the reaction, and the negative value of ∆H reveals that the interaction of the Fe complex with DNA is exothermic.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Spectroscopy, Structure, Biomacromolecular Interactions, and Antiproliferation Activity of a Fe(II) Complex With DPA-Bpy as Pentadentate Ligand

et al. 2022

View full text Add to dashboard Cite

An Fe(II) complex with DPA-Bpy (DPA-Bpy = N,N-bis(2-pyridinylmethyl)-2,20-bipyridine-6 -methanamine) as the ligand was synthesized and characterized to mimic bleomycin. The binding constants (Kb) of the complex with calf thymus DNA and human serum albumin (HSA) were quantitatively evaluated using fluorescence spectroscopy, with Kb as 5.53×105 and 2.40×104 M−1, respectively; the number of the average binding site (n) is close to 1. The thermodynamic analyses suggested that the electrostatic interactions exist between the complex and DNA, and the hydrogen bonding and Van der Waals force exist for the complex and HSA. The Fe complex exhibits cleavage ability toward pBR322 DNA, and the crystal structure of the HSA Fe complex adduct at 2.4 Å resolution clearly shows that His288 serves as the axial ligand of the Fe center complexed with a pentadentate DPA-Bpy ligand. Furthermore, the cytotoxicity of the complex was evaluated against HeLa cells. Both the Fe complex and HSA Fe complex adduct show obvious effect on cell proliferation with an IC50 of 1.18 and 0.82 μM, respectively; they induced cell apoptosis and arrested cell cycles at S phase. This study provides insight into the plausible mechanism underlying their metabolism and pharmacological activity.

show abstract

…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Spectroscopy, Structure, Biomacromolecular Interactions, and Antiproliferation Activity of a Fe(II) Complex With DPA-Bpy as Pentadentate Ligand

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Under the experimental used conditions, the presence of 2.00 μM Lyz, OVA, PA, and PC resulted in the errors of -0.016, 0.025, -0.0092, and 0.014 μM in BHb determination, respectively, which was only about -7.1 % ∼11 % of the interference levels in the normal ratiometric fluorescence method. Among the six proteins test, PC solution emits weak fluorescence in the visible spectral region of 600-780 nm peak at 672 nm with the excitation wavelength of 532 nm [49]. But the fluorescence from low concentration PC solution is much weaker than that from CdTe QDs in MIPs and NIPs.…”

Section: Sensitivity and Selectivity Of Mips Sensor For Bhb Determina...mentioning

confidence: 98%

Enhancing anti-interference ability of molecularly imprinted ratiometric fluorescence sensor via differential strategy demonstrated by the detection of bovine hemoglobin

Wang

Chen

Wang

et al. 2020

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…19 Furthermore, PL and LIF spectroscopies have recently been employed to study drug-protein interactions. 20,21 In the present study, fluorescence properties for different Xeloda concentrations were assessed using PL measurements. Figure 4a depicts the Xeloda fluorescence spectra for various concentrations ranging from 0.007 to 10 mg/mL.…”

Section: Pl Spectramentioning

confidence: 99%

Spectroscopic Characteristics of Xeloda Chemodrug

et al. 2021

View full text Add to dashboard Cite

Introduction: Spectroscopic properties of Xeloda chemodrug have been studied over varying concentrations ranging between 0.001 and 10 mg/mL, using laser-induced fluorescence (LIF) spectroscopy. The alternative photoluminescence (PL) and near infrared (NIR) measurements are carried out to authenticate the obtained results by the LIF method. Methods: The XeCl laser as the excitation coherent source with 160 mJ/pulse at 308 nm is employed for LIF measurements of the fluorophore of interest in the modular spectroscopic set-up. Results: Xeloda as a significant chemodrug acts as a notable fluorophore. LIF, PL and NIR spectroscopy techniques are employed to investigate the spectral properties of the chemodrug in terms of concentration. The maximum LIF peak intensity of Xeloda is achieved at λmax=410.5 nm and the characteristic concentration of CP1=0.05 mg/mL. PL signals are in good agreement with the data given by the LIF measurements. The characteristic NIR spectra of Xeloda as solid evidence of chemical bonding formation attest to fluorescence quenching at the fluorophore concentration of ~ 0.2 mg/ mL. Besides, the spectral shift of fluorescence signals which is obtained in terms of fluorophore concentration- demonstrating as a diagnostic marker for the purpose of optimized chemotherapy. Conclusion: Xeloda exhibits outstanding fluorescence properties over the allowable concentration in human serum (Cmax). These characteristics could benefit potential advantage of simultaneous laser-based imaging of cell-chemodrug interaction over in-vivo studies.

show abstract

Fluorescence properties of Phycocyanin and Phycocyanin-human serum albumin complex

Cited by 34 publications

References 28 publications

Spectroscopy, Structure, Biomacromolecular Interactions, and Antiproliferation Activity of a Fe(II) Complex With DPA-Bpy as Pentadentate Ligand

Spectroscopy, Structure, Biomacromolecular Interactions, and Antiproliferation Activity of a Fe(II) Complex With DPA-Bpy as Pentadentate Ligand

Enhancing anti-interference ability of molecularly imprinted ratiometric fluorescence sensor via differential strategy demonstrated by the detection of bovine hemoglobin

Spectroscopic Characteristics of Xeloda Chemodrug

Contact Info

Product

Resources

About