Fluorescence Properties and Metabolic Features of Indocyanine Green (ICG) as Related to Angiography

Desmettre, Thomas; Devoisselle, Jean-Marie; Mordon, Serge

doi:10.1016/s0039-6257(00)00123-5

Cited by 643 publications

(587 citation statements)

References 66 publications

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“…ICG is a US Food and Drug Administration (FDA)-approved NIRF dye that does not show any independent propensity for targeting neoplastic tissue as seen in renal cell carcinoma, mostly by perfusion and diffusion differences or neoplastic and normal tissue (washout). ICG is in clinical use to visualize vasculature or lymphatics (18)(19)(20). ICG has a low level of fluorescence in aqueous solution, whereas its emission increases on binding to hydrophobic pockets of proteins (such as albumin) or cellular membranes.…”

Section: Discussionmentioning

confidence: 99%

Targeted imaging of urothelium carcinoma in human bladders by an ICG pHLIP peptide ex vivo

Golijanin

Amin

Moshnikova

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Bladder cancer is the fifth most common in incidence and one of the most expensive cancers to treat. Early detection greatly improves the chances of survival and bladder preservation. The pH low insertion peptide (pHLIP) conjugated with a near-infrared fluorescent dye [indocyanine green (ICG)] targets low extracellular pH, allowing visualization of malignant lesions in human bladder carcinoma ex vivo. Cystectomy specimens obtained after radical surgery were immediately irrigated with nonbuffered saline and instilled with a solution of the ICG pHLIP construct, incubated, and rinsed. Bladders were subsequently opened and imaged, the fluorescent spots were marked, and a standard pathological analysis was carried out to establish the correlation between ICG pHLIP imaging and white light pathological assessment. Accurate targeting of bladder lesions was achieved with a sensitivity of 97%. Specificity is 100%, but reduced to 80% if targeting of necrotic tissue from previous transurethral resections or chemotherapy are considered as false positives. The ICG pHLIP imaging agent marked high-grade urothelial carcinomas, both muscle invasive and nonmuscle invasive. Carcinoma in situ was accurately diagnosed in 11 cases, whereas only four cases were seen using white light, so imaging with the ICG pHLIP peptide offers improved early diagnosis of bladder cancers and may also enable new treatment alternatives.bladder cancer | fluorescence-guided surgery | NIR imaging | acidity | human tissue

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Section: Discussionmentioning

confidence: 99%

Targeted imaging of urothelium carcinoma in human bladders by an ICG pHLIP peptide ex vivo

Golijanin

Amin

Moshnikova

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…Indocianine green (ICG) is a well-known, relatively harmless dye for IR blood angiography and IR imaging of lymph nodes in human and animals [196][197][198][199][200][201] . Within the first seconds after intravenous injection, there is a shift in the absorption spectrum of ICG from 780 nm to 805 nm (spectral stabilization) due to two processes: polymerization of ICG molecules and their binding to plasma proteins and lipoproteins [196] .…”

Section: Ir Angiography and Lymphgraphymentioning

confidence: 99%

“…Within the first seconds after intravenous injection, there is a shift in the absorption spectrum of ICG from 780 nm to 805 nm (spectral stabilization) due to two processes: polymerization of ICG molecules and their binding to plasma proteins and lipoproteins [196] . Plasma clearance of ICG is biphasic, showing a rapid first phase with a half-life of 4-6 min and a secondary phase with a half-life of more than 1 h [197] .…”

Section: Ir Angiography and Lymphgraphymentioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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“…A ICG representa o corante mais apropriado para mostrar vasculatura coroidal em exames de contraste. A ICG mostra grande afinidade aos componentes da matriz extracelular da membrana limitante interna (MLI) facilitando sua remoção (3) . Cromovitrectomia com ICG ga-nhou aceitação mundial quando mostrou tornar a cirurgia de buraco macular bem mais facilitada (4) .…”

Section: Indocianina Verde (Icg)unclassified

Corantes vitais em cromovitrectomia

Dib¹,

Rodrigues²,

Maia³

et al. 2009

Arq. Bras. Oftalmol.

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Fluorescence Properties and Metabolic Features of Indocyanine Green (ICG) as Related to Angiography

Cited by 643 publications

References 66 publications

Targeted imaging of urothelium carcinoma in human bladders by an ICG pHLIP peptide ex vivo

Targeted imaging of urothelium carcinoma in human bladders by an ICG pHLIP peptide ex vivo

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Corantes vitais em cromovitrectomia

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