Fluorescence polarisation changes in lymphocyte cytoplasm as a diagnostic test for breast carcinoma

Ron, Idit; Deutsch, Mordechai; Tirosh, Reuven; Weinreb, A.; Eisenthal, Avi; Chaitchik, Samario

doi:10.1016/0959-8049(95)90535-9

Cited by 17 publications

(13 citation statements)

References 17 publications

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“…Among the neoplasms studied were colorectal cancer , breast cancer (Rahmani et al 1996, Ron et al 1995, Klein et al 2002a, 2002b, prostate cancer (Klein et al 1999), malignant melanoma (Merimsky et al 1997), lung and ovarian cancer . In these studies, diagnostic algorithms have been created, based on PBLs responses to different antigenic stimuli.…”

Section: Early Cancer Detectionmentioning

confidence: 99%

“…No significant stage-related differences were found in the polarization values following stimulation by EF or PHA in breast cancer patients . Also used were CaBr (Ron et al 1995) and MUC-1/SEC (Klein et al 2002a(Klein et al , 2002b, both tumor-associated antigens specific for breast cancer, PSA-ACT, a prostate specific antigen (Klein et al 1999), MEL, a melanoma antigen, as well as different tumor antigen extracts (TAE) , Rahmani et al 1996.…”

Section: Early Cancer Detectionmentioning

confidence: 99%

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Application of a Static Fluorescence‐based Cytometer (the CellScan) in Basic Cytometric Studies, Clinical Pharmacology, Oncology and Clinical Immunology

Harel

Gilburd

Schiffenbauer

et al. 2005

Journal of Immunology Research

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The CellScan apparatus is a laser scanning cytometer enabling repetitive fluorescence intensity (FI) and polarization (FP) measurements in living cells, as a means of monitoring lymphocyte activation. The CellScan may serve as a tool for diagnosis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) as well as other autoimmune diseases by monitoring FP changes in peripheral blood lymphocytes (PBLs) following exposure to autoantigenic stimuli. Changes in FI and FP in atherosclerotic patients' PBLs following exposure to various stimuli have established the role of the immune system in atherosclerotic disease. The CellScan has been evaluated as a diagnostic tool for drug-allergy, based on FP reduction in PBLs following incubation with allergenic drugs. FI and FP changes in cancer cells have been found to be well correlated with the cytotoxic effect of anti-neoplastic drugs. In conclusion, the CellScan has a variety of applications in cell biology, immunology, cancer research and clinical pharmacology.

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Section: Early Cancer Detectionmentioning

confidence: 99%

Section: Early Cancer Detectionmentioning

confidence: 99%

Application of a Static Fluorescence‐based Cytometer (the CellScan) in Basic Cytometric Studies, Clinical Pharmacology, Oncology and Clinical Immunology

Harel

Gilburd

Schiffenbauer

et al. 2005

Journal of Immunology Research

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“…The estimation of the kinetics of this process was conducted via the Michaelis-Menten constants. Our preliminary results have shown that early activation events, which occur upon cell incubation with and without either phytohemagglutinin or autologous tumor tissue, may be demonstrated by the general biochemical kinetic parameters (the Michaelis-Menten constant, K m , and the maximal velocity, V max ) that display the differences in intracellular processes in the lymphocytes, related to the enzymatic activity as well as to the cell size, the cytoplasm membrane permeability, and so forth (15,16,19).…”

Section: Introductionmentioning

confidence: 99%

“…15,19). The estimation of the kinetics of this process was conducted via the Michaelis-Menten constants.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Intracellular Enzyme Kinetic Characteristics of Peripheral Mononuclear Cells in Breast Cancer Patients

Afrimzon

Zurgil

Shafran

et al. 2004

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Early activation responses of human peripheral lymphocytes as induced by mitogenic or antigenic stimulation can be detected by a characteristic decrease in IFFP (3,8,9,13,21). Changes in IFFP were also evident in fibroblasts following cytokine exposure (15).…”

mentioning

confidence: 99%

Indication that Intracellular Fluorescence Polarization of T Lymphocytes is Cell Cycle Dependent.

Zurgil

Deutsch

Tirosh

et al. 1996

Cell Struct. Funct.

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ABSTRACT. The degree of depolarization of fluorescence light emitted from an organic dye, used as a molecular probe, is a powerful tool in probing the microenvironment. Polarization measurements of intracellular exogenous fluorescein have been shown to reflect the physiological state of the cells. The relationship between intracellular fluorescein fluorescence polarization (IFFP) and cell cycle, was investigated in the leukemia T-lymphocyte Jurkat cell line. Jurkat T cells were cultured in increasing cell densities, their cell cycle progression cytometrically monitored and the IFFP measured. At the highest cell density, the subpopulation of cells at the resting phases the (G0/Gi) predominated, and the mean IFFP was 0.186 ±0.015. At the lowest density, with diminished proportion of cells in the Gi/G2 stages the mean IFFP decreased to 0. sis, Ca+2 mobilization and the induction of early protooncogenes. These events are followed by the increased expression of the genes for interleukine IL-2 and its receptor, leading to cell proliferation. Stimulation of T lymphocytes with PHAhas also

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Fluorescence polarisation changes in lymphocyte cytoplasm as a diagnostic test for breast carcinoma

Cited by 17 publications

References 17 publications

Application of a Static Fluorescence‐based Cytometer (the CellScan) in Basic Cytometric Studies, Clinical Pharmacology, Oncology and Clinical Immunology

Application of a Static Fluorescence‐based Cytometer (the CellScan) in Basic Cytometric Studies, Clinical Pharmacology, Oncology and Clinical Immunology

Monitoring of Intracellular Enzyme Kinetic Characteristics of Peripheral Mononuclear Cells in Breast Cancer Patients

Indication that Intracellular Fluorescence Polarization of T Lymphocytes is Cell Cycle Dependent.

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