Fluorescence of experimental atheromatous plaques with hematoporphyrin derivative.

Spears, J. Richard; Serur, Juan R.; Shropshire, D; Paulin, Sven

doi:10.1172/jci110782

Cited by 148 publications

(48 citation statements)

References 19 publications

(21 reference statements)

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“…However, it is generally considered that photosensitizers are taken up by proliferating cells, in which DNA synthesis is accelerated, such as cancer cells and migrating SMCs. [9][10][11] Accumulation of HPD was confirmed to coincide with PCNA-positive cells in the intima and media of the injured arteries in the present study. However, as shown by the fact that peak accumulation of HPD in the media occurred 1 week after the vascular injury whereas the peak number of PCNA-positive cells in the media was observed on day 3 after the vascular injury, there was no steady correlation between the amount of HPD accumulated and the number of proliferating SMCs (Fig 2).…”

Section: Hpd Accumulationsupporting

confidence: 77%

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Photodynamic Therapy for the Prevention of Intimal Hyperplasia in Balloon-Injured Rabbit Arteries

et al. 1999

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Section: Hpd Accumulationsupporting

confidence: 77%

“…[9][10][11] The photosensitizer remains dormant until it is activated by light of a very specific wavelength. The light that activates the compound is produced by a light source and is often transmitted through specially modified fiber optics.…”

mentioning

confidence: 99%

Photodynamic Therapy for the Prevention of Intimal Hyperplasia in Balloon-Injured Rabbit Arteries

et al. 1999

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“…Although only fibro-fatty atheromas were studied, preferential absorption would (3), or photosensitizers such as hematoporphyrin derivative (46). In general, however, it seems most reasonable to exploit existing absorbances when possible.…”

Section: Methodsmentioning

confidence: 99%

Preferential light absorption in atheromas in vitro. Implications for laser angioplasty.

Prince¹,

Deutsch²,

Mathews-Roth³

et al. 1986

J. Clin. Invest.

131

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Laser angioplasty, the in situ ablation of arterial obstructions with laser radiation, has been demonstrated in animal models and early clinical trials. A problem with this technique, however, is the possibility of thermal damage to adjacent or underlying normal tissues that also absorb the radiation. Using a spectrophotometer with an integrating sphere and a specially constructed tunable-dye laser-based spectrophotometer, we evaluated the transmittance and remittance of human cadaveric atheromas and adjacent normal aorta from 250 to 1,300 unm to identify wavebands where there is preferential light absorption by atheromas. Data were analyzed by both the Kubelka-Munk formalism and a Beer's law model. Both methods indicate that atheromas absorb more than normal aorta between 420 and 530 nm. At 470 nm the average Kubelka-Munk absorption coefficient of atheromas from 10 cadavers was 54±9 cm-1 compared with 26±6 cm-' for normal aortic specimens from seven cadavers. Yellow chromophores responsible for the atheroma absorbance were extractable with xylenes. Thin-layer chromatography and absorption spectra identified the extracted chromophores as predominantly consisting of a mix of carotenoids, which are known constituents of atheromatous lesions. Preferential absorption of blue light by carotenoids in atheromas may permit selective ablation of atheromatous obstructions with appropriate pulses of laser radiation.

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“…Since Spears et al first reported in 1983 the accumulation of HpD in atheroma, 4 the accumulation of several photosensitizers in atherosclerosis have been studied. [24][25][26] The pattern of accumulation of HpD in atherosclerotic tissue has been reported to show the greatest red fluorescence in the lumenal side of the intima, then gradually decrease towards the media, with no medial accumulation.…”

Section: Accumulation Of Porfimer Sodium In Atheromamentioning

confidence: 99%

“…3 There have been reports of the accumulation of photosensitizer in atheroma. [4][5][6][7] Watari et al found that when HpD was administered intravenously to rabbits, it accumulated in atheromatous intima and, using fluorescent microscopy and fluorescence spectrum analysis, they confirmed that the strength of fluorescence varies with the degree of thickness of the intima. 8 There have also been reports that when PDT is performed using the argon-dye laser intravascularly or extravascularly on aortic atherosclerosis in rabbits that had been administered HpD, there is regression or exfoliation of atheromatous intima, giving an enlarged lumen.…”

mentioning

confidence: 99%

Photodynamic Therapy of Atherosclerosis Using YAG-OPO Laser and Porfimer Sodium, and Comparison With Using Argon-Dye Laser

et al. 1999

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We performed photodynamic therapy (PDT) using the Yttrium Aluminium Garnet-Optical Parametric Oscillated (YAG-OPO) laser in cases of atherosclerosis, and examined its efficacy in vivo. We also performed PDT using an Argon-dye (Ar-dye) laser with the same output, and compared the efficacies. Following balloon denudation injury of the thoracoabdominal aorta, rabbits were raised on a cholesterol diet for 16 weeks, producing atheroma in that region. At 24 h following the administration of Photofrin 5 mg/kg, PDT was performed, and animals were sacrificed at 1 day, 1 week, and 2 weeks following the procedure to examine its efficacy. This was compared with the efficacy of PDT using the Ar-dye laser. Following PDT using a YAG-OPO laser, an increase in the vessel lumen was seen due to reduction of the hypertrophic intima and media, without the appearance of inflammatory cells. This result was seen more strongly in PDT using the pulse wave YAG-OPO laser than with the continuous wave Ar-dye laser, affecting not just the intima but also the media. These data demonstrated that PDT can effectively regress atherosclerotic lesions. (Jpn Circ J 1999; 63: 288 -295)

show abstract

Fluorescence of experimental atheromatous plaques with hematoporphyrin derivative.

Cited by 148 publications

References 19 publications

Photodynamic Therapy for the Prevention of Intimal Hyperplasia in Balloon-Injured Rabbit Arteries

Photodynamic Therapy for the Prevention of Intimal Hyperplasia in Balloon-Injured Rabbit Arteries

Preferential light absorption in atheromas in vitro. Implications for laser angioplasty.

Photodynamic Therapy of Atherosclerosis Using YAG-OPO Laser and Porfimer Sodium, and Comparison With Using Argon-Dye Laser

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