Fluorescence of Candida in diagnosis of oral candidiasis

Naik, L R Kumaraswamy; Shetty, Pushparaja; Prasad, M.; Karnaker, Vimal Kumar; Shroff, Sarosh E; Madathil, Lal P

doi:10.4103/0970-9290.199592

Cited by 6 publications

(4 citation statements)

References 1 publication

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“…Furthermore, Candida fluorescence is employed in laboratory tests, enabling expedited results. An example of this is the use of a fluorescent laser with calcofluor to detect fungi [ 53 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light

Wiench,

Paliga,

Mertas

et al. 2024

Dentistry Journal

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Background: Candida albicans and similar species are significant pathogens in immunocompromised and hospitalized individuals, known for mucosal colonization and bloodstream/organ invasion. Many pathogenic fungi, including these species, exhibit autofluorescence (R/OF) under specific light conditions, a feature crucial for their detection. Aim: We investigated the use of a 405 nm diode laser for the direct observation of red/orange autofluorescence of Candida spp., common in the oral cavity, exploring its potential in health screenings. Methods: This study utilized cultures of Candida spp. on Sabouraud dextrose agar with Qdot 655 and 685 for fluorescence benchmarking, illuminated using a 405 nm diode laser (continuous wave, power 250 mW, 0.0425 J/cm² fluence, 0.0014 W/cm² power density). Images were captured using a yellow-filter camera at set intervals (48 to 144 h). Visual and computational analyses evaluated the R/OF in terms of presence, intensity, coloration, and intra-colony variation. Results: Most Candida strains displayed red/orange autofluorescence at all observation times, characterized by varied coloration and intra-colony distribution. Initially, there was an increase in R/OF intensity, which then stabilized in the later stages of observation. Conclusions: The majority of the Candida strains tested are capable of emitting R/OF under 405 nm laser light. This finding opens up new possibilities for integrating R/OF detection into routine dental screenings for Candida spp.

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Section: Discussionmentioning

confidence: 99%

Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light

Wiench,

Paliga,

Mertas

et al. 2024

Dentistry Journal

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“…The validity of fluorescence microscopy to diagnose Candida infection is also well-established [15], providing a sensitive and specific screening tool, compared to the current gold standard, i.e. periodic acid Schiff (PAS) stain [16]. Gabrielli et al compared in mice the bioluminescence in vivo imaging technique with colony forming units (CFU) measurement for the Candida detection.…”

Section: Discussionmentioning

confidence: 99%

“…They concluded that the bioluminescence technique was more reliable than CFU counts in detecting mouse’s early oral candidiasis [17]. The typical fluorescence aspect of hyphae is a yellowish-green signal, due to their flavin content [16].…”

Section: Discussionmentioning

confidence: 99%

Dorsal tongue porphyrin autofluorescence and Candida saprophytism: A prospective observational study

et al. 2019

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AimTo investigate the correlation between the dorsal tongue porphyrin autofluorescence, revealed using VELscope, and Candida saprophytism.Material and methodsConsecutive patients underwent an autofluorescence examination by the VELscope device to establish the presence or absence of porphyrin fluorescence. A tongue swab was collected for the Candida cultural test. Sensitivity, specificity, accuracy, negative predictive value and positive predictive value were calculated considering the oral swab as the gold standard. The degree of agreement between the two tests was calculated using Cohen's K coefficient.ResultsOne hundred twenty-six patients were enrolled. Porphyrin fluorescence method showed a sensitivity of 78%, specificity of 76% and an accuracy of 78%. Negative predictive value and positive predictive value were respectively 90% and 59%. The strength of agreement between the two methods resulted to be moderate (k = 0.551).ConclusionsOff-label use of tongue autofluorescence examination to detect the presence of Candida species is characterized by a loss of porphyrin fluorescence. The high negative predictive value of porphyrin fluorescence loss suggests its use in preliminary selection of Candida carriers, in order to plan preventive and therapeutic strategies.

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“…Diagnosis of oral candidiasis in smears and swabs is rapidly performed by fungal fluorescent staining as well as with periodic acid-Schiff stain and observation under a fluorescence microscope [60]. The pathogen is identified by blue fluorescence surrounding its annular or tubular structures [61].…”

Section: Optical Biosensormentioning

confidence: 99%

Innovative Biosensing Approaches for Swift Identification of Candida Species, Intrusive Pathogenic Organisms

Lorenzo-Villegas,

Gohil,

Lamo

et al. 2023

Life

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Candida is the largest genus of medically significant fungi. Although most of its members are commensals, residing harmlessly in human bodies, some are opportunistic and dangerously invasive. These have the ability to cause severe nosocomial candidiasis and candidemia that affect the viscera and bloodstream. A prompt diagnosis will lead to a successful treatment modality. The smart solution of biosensing technologies for rapid and precise detection of Candida species has made remarkable progress. The development of point-of-care (POC) biosensor devices involves sensor precision down to pico-/femtogram level, cost-effectiveness, portability, rapidity, and user-friendliness. However, futuristic diagnostics will depend on exploiting technologies such as multiplexing for high-throughput screening, CRISPR, artificial intelligence (AI), neural networks, the Internet of Things (IoT), and cloud computing of medical databases. This review gives an insight into different biosensor technologies designed for the detection of medically significant Candida species, especially Candida albicans and C. auris, and their applications in the medical setting.

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Fluorescence of Candida in diagnosis of oral candidiasis

Abstract: PAP autofluorescence is less sensitive than PAS, still it accentuates the distinct morphological features of Candida.

Cited by 6 publications

References 1 publication

Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light

Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light

Dorsal tongue porphyrin autofluorescence and Candida saprophytism: A prospective observational study

Innovative Biosensing Approaches for Swift Identification of Candida Species, Intrusive Pathogenic Organisms

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