Fluorescence of 1,2‐Diaminoanthraquinone and its Nitric Oxide Reaction Product within Macrophage Cells

Abstract

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Resolution of Cutaneous Leishmaniasis and Persistence ofLeishmania majorin the Absence of Arginase 1

“…In situ detection of NO was done as described (22). Briefly, unfixed frozen tissue sections were stained with 10 mM 1,2-diaminoanthraquinone (DAQ; Sigma-Aldrich) in PBS with 10% DMSO at 37˚C for 45 min, followed by extensive washing steps (63,64).…”

Imaging of compartmentalised intracellular nitric oxide, induced during bacterial phagocytosis, using a metalloprotein–gold nanoparticle conjugate

“…Macrophages are integral to the immune response and when activated by a foreign body increase their production of NO. 38 The external stimulation of RAW264.7γ NO – cells to produce NO requires both interferon-γ (IFN-γ) and lipopolysaccharide (LPS) for full activation, making its behaviour more typical of primary macrophages. 39 The sensing capability of the NO nanobiosensor was evaluated using cells that had been incubated overnight with the nanobiosensor and treated under four different stimulation conditions to produce varying amounts of NO: (1) unstimulated (control) cells; (2) stimulated with IFN-γ alone; (3) stimulated with both IFN-γ and LPS; and (4) stimulated with both IFN-γ and LPS together with an inhibitor of iNOS, N ω -nitro- l -arginine methyl ester hydrochloride ( l -NAME).…”

Nitric oxide release by N-(2-chloroethyl)-N-nitrosoureas: a rarely discussed mechanistic path towards their anticancer activity

“…62-64 1,2-Diamino anthraquinone (DAA) may be used as a sensitive and specic NO probe. 65 1,2-Diamino compounds react with nitric oxide (NO) in presence of O 2 to produce a uorescent triazole derivative (DAA-Tz) (Scheme S2, ESI †) with a new emission maxima. 65 The emission spectra of DAA in DMSO (50 mM) exhibit peak maxima at 638 nm with a weak shoulder at 570 nm (Fig.…”