Fluorescence nanoscopy by ground-state depletion and single-molecule return

Fölling, Jonas; Bossi, Mariano L.; Bock, Hannes; Medda, Rebecca; Wurm, Christian A.; Hein, Birka; Jakobs, Stefan; Eggeling, Christian; Hell, Stefan W.

doi:10.1038/nmeth.1257

Cited by 712 publications

(714 citation statements)

References 16 publications

(27 reference statements)

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“…The localization of fluorescence particles was determined by fitting single molecule fluorescence signals with a 2D Gaussian function using LASAF software (Leica). The localization accuracy (i.e., standard error) of the system is limited by the statistical noise of photon counting (Heisenberg, 1930;Fölling et al, 2008;Schermelleh et al, 2010). Thus, assuming the point-spread functions are Gaussian, the precision of localization is proportional to DLR/√N, where DLR is the diffraction-limited resolution of a fluorophore and N is the number of detected photons.…”

Section: Frapmentioning

confidence: 99%

Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle

Mercado¹,

Baylie²,

Navedo³

et al. 2014

J Cell Biol

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Section: Frapmentioning

confidence: 99%

Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle

Mercado¹,

Baylie²,

Navedo³

et al. 2014

J Cell Biol

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“…10 Called GSDIM, 11 this method requires all but a single NV center within about a 250 nm range to be dark, in turn requiring tight control of dark state occupation, i.e., of the 'blinking'. In reality, it is difficult to control blinking such to ensure that at most a single NV center is signaling within a 250 nm spatial range.…”

mentioning

confidence: 99%

Stimulated Emission Depletion Microscopy Resolves Individual Nitrogen Vacancy Centers in Diamond Nanocrystals

et al. 2013

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Nitrogen-vacancy (NV) color centers in nanodiamonds are highly promising for bioimaging and sensing. However, resolving individual NV centers within nanodiamond particles and the controlled addressing and readout of their spin state has remained a major challenge. Spatially stochastic superresolution techniques cannot provide this capability in principle, whereas coordinate-controlled super-resolution imaging methods, like stimulated emission depletion (STED) microscopy, have been predicted to fail in nanodiamonds.Here we show that, contrary to these predictions, STED can resolve single NV centers in 40À250 nm sized nanodiamonds with a resolution of ≈10 nm. Even multiple adjacent NVs located in single nanodiamonds can be imaged individually down to relative distances of ≈15 nm. Far-field optical super-resolution of NVs inside nanodiamonds is highly relevant for bioimaging applications of these fluorescent nanolabels. The targeted addressing and readout of individual NV À spins inside nanodiamonds by STED should also be of high significance for quantum sensing and information applications.

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“…Then the accuracy of localisation of the molecule can be smaller than the pixel resolution of the camera. 7 This principle has recently been extended by the development of localisation-based super-resolution microscopy techniques such as PALM [27,28] and STORM/GSDIM [29,30].…”

Section: Resolutionmentioning

confidence: 99%

Single-molecule fluorescence imaging by total internal reflection fluorescence microscopy (IUPAC Technical Report)

Knight

2014

Pure and Applied Chemistry

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Total internal reflection fluorescence (TIRF) is a popular illumination technique in microscopy, with many applications in cell and molecular biology and biophysics. The chief advantage of the technique is the high contrast that can be achieved by restricting fluorescent excitation to a thin layer. We summarise the optical theory needed to understand the technique and various aspects required for a practical implementation of it, including the merits of different TIRF geometries. Finally, we discuss a variety of applications including super-resolution microscopy and high-throughput DNA sequencing technologies.

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Fluorescence nanoscopy by ground-state depletion and single-molecule return

Cited by 712 publications

References 16 publications

Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle

Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle

Stimulated Emission Depletion Microscopy Resolves Individual Nitrogen Vacancy Centers in Diamond Nanocrystals

Single-molecule fluorescence imaging by total internal reflection fluorescence microscopy (IUPAC Technical Report)

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