Fluorescence molecular tomography in the presence of background fluorescence

Soubret, Antoine; Ntziachristos, Vasilis

doi:10.1088/0031-9155/51/16/007

Cited by 63 publications

(80 citation statements)

References 53 publications

(50 reference statements)

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“…Background subtraction was performed as previously described. 19 The third technique involved the acquisition of an additional background image for each source employed, collected through the matching medium at the emission wavelength with the mouse removed. The background image was used to calculate the attenuation-corrected image (ACI) by dividing each fluorescence image by an attenuation-corrected ratio (image collected through mouse at the excitation wavelength/background image), and then summing all of the 46 processed images.…”

Section: Image Processingmentioning

confidence: 99%

Fluorescence Tomography and Magnetic Resonance Imaging of Myocardial Macrophage Infiltration in Infarcted Myocardium In Vivo

et al. 2007

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Background-Fluorescence imaging of the heart is currently limited to invasive ex vivo or in vitro applications. We hypothesized that the adaptation of advanced transillumination and tomographic techniques would allow noninvasive fluorescence images of the heart to be acquired in vivo and be coregistered with in vivo cardiac magnetic resonance images. Methods and Results-The uptake of the magnetofluorescent nanoparticle CLIO-Cy5.5 by macrophages in infarcted myocardium was studied. Ligation of the left coronary artery was performed in 12 mice and sham surgery in 7. The mice were injected, 48 hours after surgery, with 3 to 20 mg of iron per kilogram of CLIO-Cy5.5. Magnetic resonance imaging and fluorescence molecular tomography were performed 48 hours later. An increase in magnetic resonance imaging contrast-to-noise ratio, indicative of myocardial probe accumulation, was seen in the anterolateral walls of the infarcted mice but not in the sham-operated mice (23.0Ϯ2.7 versus 5.43Ϯ2.4; PϽ0.01). Fluorescence intensity over the heart was also significantly greater in the fluorescence molecular tomography images of the infarcted mice (19.1Ϯ5.2 versus 5.3Ϯ1.4; PϽ0.05). The uptake of CLIO-Cy5.5 by macrophages infiltrating the infarcted myocardium was confirmed by fluorescence microscopy and immunohistochemistry. Conclusions-Noninvasive imaging of myocardial macrophage infiltration has been shown to be possible by both fluorescence tomography and magnetic resonance imaging. This could be of significant value in both the research and clinical settings. The techniques developed could also be used to image other existing fluorescent and magnetofluorescent probes and could significantly expand the role of fluorescence imaging in the heart. (Circulation. 2007;115: 1384-1391.)Key Words: fluorescence Ⅲ inflammation Ⅲ magnetic resonance imaging Ⅲ myocardium Ⅲ tomography F luorescence-based imaging techniques have become an integral component of modern cardiovascular research. However, with the exception of a few landmark studies using intravital microscopy or photographic imaging of the exposed heart, 1-3 cardiac fluorescence imaging has generally been limited to ex vivo and in vitro applications. 4,5 In the present study, we describe the application of advanced transillumination and tomographic techniques to allow fluorescence imaging of the heart to be performed noninvasively in vivo. In particular, deep-seated fluorescence activity in the heart is resolved with the use of fluorescence molecular tomography (FMT), a technique developed for 3-dimensional and quantitative fluorescence imaging. 6,7 Clinical Perspective p 1391The feasibility and value of cardiac FMT are demonstrated in the present study in a murine model of postinfarction myocardial macrophage infiltration. Postinfarction injury is, at present, often assessed by the retention of gadolinium within the myocardium by magnetic resonance imaging (MRI). 8 MRI agents targeted to specific molecular processes such as cardiomyocyte apoptosis have also been developed recently...

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Section: Image Processingmentioning

confidence: 99%

Fluorescence Tomography and Magnetic Resonance Imaging of Myocardial Macrophage Infiltration in Infarcted Myocardium In Vivo

et al. 2007

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“…We assume that 1) The endogenous absorption coefficients at the emission and the excitation wavelengths are approximately equal, i.e., . This is a valid assumption for a number of different applications such as small animal and breast imaging [33].…”

Section: B Nonlinear Concentration-to-measurement Mapmentioning

confidence: 99%

“…To do so, we first concatenate the concentration vectors and the parameter vectors for all voxels, and form the following vectors: We next utilize the EKF framework to estimate the fluorophore concentration images in different compartments and pharmacokinetic-rate images based on (30)- (33). Table I tabulates the steps of the EKF algorithm.…”

Section: B Estimation Of Pharmacokinetic-rate Images By Extended Kalmentioning

confidence: 99%

Direct Reconstruction of Pharmacokinetic-Rate Images of Optical Fluorophores From NIR Measurements

Alacam

Yazıcı

2009

IEEE Trans. Med. Imaging

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Abstract-In this paper, we present a new method to form pharmacokinetic-rate images of optical fluorophores directly from near infra-red (NIR) boundary measurements. We first derive a mapping from spatially resolved pharmacokinetic rates to NIR boundary measurements by combining compartmental modeling with a diffusion based NIR photon propagation model. We express this mapping as a state-space equation. Next, we introduce a spatio-temporal prior model for the pharmacokinetic-rate images and combine it with the state-space equation. We address the image formation problem using the extended Kalman filtering framework. We analyze the computational complexity of the resulting algorithms and evaluate their performance in numerical simulations. An important feature of our approach is that the reconstruction of fluorescence concentrations and compartmental modeling are combined into a single step 1) to take advantage of the inherent temporal correlations in dynamic NIR measurements, and 2) to incorporate spatio-temporal a priori information on pharmacokinetic-rate images. Simulation results show that the resulting algorithms are more robust and lead to higher signal-to-noise ratio as compared to existing approaches where the reconstruction of concentrations and compartmental modeling are treated separately. Additionally, we reconstructed pharmacokinetic-rate images using in vivo data obtained from three patients with breast tumors. The reconstruction results show that the pharmacokinetic rates of indocyanine green are higher inside the tumor region as compared to the surrounding tissue.

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“…Based on the imaging and reconstruction model, the distribution and concentration of the nanophosphors inside imaging object can be resolved. Compared with other optical molecular imaging such as bioluminescence tomography (BLT) [5][6][7] and fluorescence molecular tomography (FMT) [8][9][10], XLCT has several advantages with the use of X-rays. Firstly, X-ray can penetrate the imaging object easily and provide more information deep inside the object.…”

Section: Introductionmentioning

confidence: 99%

Resolving adjacent nanophosphors of different concentrations by excitation-based cone-beam X-ray luminescence tomography

Gao

Rong

et al. 2017

Biomed. Opt. Express

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Cone-beam X-ray luminescence computed tomography (CB-XLCT) has been proposed as a new molecular imaging modality recently. It can obtain both anatomical and functional tomographic images of an object efficiently, with the excitation of nanophosphors in vivo or in vitro by cone-beam X-rays. However, the ill-posedness of the CB-XLCT inverse problem degrades the image quality and makes it difficult to resolve adjacent luminescent targets with different concentrations, which is essential in the monitoring of nanoparticle metabolism and drug delivery. To address this problem, a multi-voltage excitation imaging scheme combined with principal component analysis is proposed in this study. Imaging experiments performed on physical phantoms by a custom-made CB-XLCT system demonstrate that two adjacent targets, with different concentrations and an edge-to-edge distance of 0 mm, can be effectively resolved. and l2-norm for two imaging models of fluorescence molecular tomography: a comparative study," J.

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Fluorescence molecular tomography in the presence of background fluorescence

Cited by 63 publications

References 53 publications

Fluorescence Tomography and Magnetic Resonance Imaging of Myocardial Macrophage Infiltration in Infarcted Myocardium In Vivo

Fluorescence Tomography and Magnetic Resonance Imaging of Myocardial Macrophage Infiltration in Infarcted Myocardium In Vivo

Direct Reconstruction of Pharmacokinetic-Rate Images of Optical Fluorophores From NIR Measurements

Resolving adjacent nanophosphors of different concentrations by excitation-based cone-beam X-ray luminescence tomography

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