Fluorescence microtomography: external mapping of elements inside biological samples

Schroer, Christian G.; Tuemmler, Johannes; Guenzler, Til Florian; Lengeler, B.; Schroeder, Walter H.; Kuhn, Arnd J.; Simionovici, Alexandre; Snigirev, A.; Snigireva, Irina

doi:10.1117/12.410573

Cited by 14 publications

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“…Details on the tomographic model as well as the reconstruction technique can be found in. 26,27 Fig. 6 shows the reconstructed distributions of potassium (K), iron (Fe), zinc (Zn), and the attenuation coefficient µ 0 at E 0 = 19.7keV on a slice through the root.…”

Section: Fluorescence Microtomography Of a Mycorrhizal Root Of The Tomentioning

confidence: 99%

“…Details of this method and about this experiment can be found in. 25,26,17 The sample was scanned horizontally through the beam in 105 steps of 1µm to obtain one tomographic projection. The fluorescence signal emanating from the plant was recorded by an energy dispersive detector (SiLi) during 2s at each step, as well as the primary and transmitted intensity by two PIN-diodes.…”

Section: Fluorescence Microtomography Of a Mycorrhizal Root Of The Tomentioning

confidence: 99%

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<title>Microbeam production using compound refractive lenses: beam characterization and applications</title>

et al. 2001

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Parabolic compound refractive lenses (PCRLs) are high quality hard x-ray imaging optics that can be used to image a synchrotron source onto a sample in a strongly demagnifying setup. This allows to produce an intensive microbeam with lateral extensions in the (sub-)micrometer range. Aluminium PCRLs can be operated in an energy range from about 10keV to 60keV and withstand the high heat load of the white beam of an ESRF undulator source. The microbeam properties using monochromatic and single undulator harmonic ("pink") radiation are discussed, focusing on beam size, depth of field, background, flux, and gain. The large depth of focus allows to scan fairly large samples (a few millimeters in thickness) with a beam of constant lateral extension. This makes tomographic scanning techniques, such as fluorescence microtomography possible. As applications, fluorescence microtomography of plant samples with sub-cellular resolution and the mapping of trace elements in single cancer cells is shown.

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Section: Fluorescence Microtomography Of a Mycorrhizal Root Of The Tomentioning

confidence: 99%

Section: Fluorescence Microtomography Of a Mycorrhizal Root Of The Tomentioning

confidence: 99%

<title>Microbeam production using compound refractive lenses: beam characterization and applications</title>

et al. 2001

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“…Therefore, the depth of focus is about 4 orders of magnitude larger than its lateral extension and the lateral extension of the microbeam is constant over a sample of about 1mm in thickness. 9 To obtain sufficient information for tomographic reconstruction from each part of the sample, it is important that the fluorescence radiation from that part can reach the detector with a sufficiently large number of counts from all perspectives, i. e., an angular interval that spans at least 180 • . To assure this, it is favorable to acquire the tomographic projections over a full rotation rather than over 180 • only.…”

Section: Influence Of Attenuation Inside the Samplementioning

confidence: 99%

High-resolution element mapping inside biological samples using fluorescence microtomography

et al. 2002

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Sample preparation for element analysis of many biological tissues is difficult to achieve and prone to introduce contamination. Using x-ray fluorescence element microtomography (XFEMT) the element distribution on a virtual section across the sample can be determined with a resolution in the micrometer range. Fluorescence microtomograms of two plant samples are shown, demonstrating the possibility to map physiologically relevant ions, trace elements, and heavy metal pollutants at the cellular level. Attenuation effects inside the plant are corrected by a self-consistent tomographic reconstruction technique.

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“…A detailed description of the experiment carried out at ID18 of the ESRF has been previously given. 9 A monochromatic microbeam (E = 19.5keV) with lateral dimensions of 6µm horizontally and 1.4µm vertically was used to scan the sample with an incident flux above 10 10 ph/s. The fluorescence radiation was recorded using a HPGe detector with an aperture of 7mm 2 positioned at 90 • to and about 18mm away from the microbeam.…”

Section: Example 1: Root Of the Mahogany Plant (Swietenia Macrophylla )mentioning

confidence: 99%

“…3 The combination with x-ray microprobe techniques has only recently been implemented at the Synchrotron. [6][7][8][9] A complementary method is differential absorption microtomography 10 that uses the strong variations in photo absorption across an absorption edge of a particular element.…”

Section: Introductionmentioning

confidence: 99%

High resolution element mapping inside biological samples using fluorescence microtomography

et al. 2003

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Fluorescence microtomography: external mapping of elements inside biological samples

Cited by 14 publications

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<title>Microbeam production using compound refractive lenses: beam characterization and applications</title>

<title>Microbeam production using compound refractive lenses: beam characterization and applications</title>

High-resolution element mapping inside biological samples using fluorescence microtomography

High resolution element mapping inside biological samples using fluorescence microtomography

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