Fluorescence Measurements of Ethidium Binding to DNA

Strothkamp, Kenneth G.; Stothkamp, Ronald E.

doi:10.1021/ed071p77

Cited by 112 publications

(67 citation statements)

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“…[29] The value obtained is lower by around 100 times than those reported for typical classical intercalators [e.g., ethidium bromide (EB)-DNA, ca. 10 6  -1 ], [30,31] about an order lower than those of the affinities of intercalators that contain similar planar ligands. [25][26][27][28] The lower K b value observed for the present complex implies that it does not intercalate very strongly or deeply between the DNA base pairs.…”

Section: Uv Titrationmentioning

confidence: 99%

Synthesis, Crystal Structure, DNA Binding, and Hydrolytic Cleavage Activity of a Manganese(II) Complex

Qian

et al. 2010

Eur J Inorg Chem

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N-bis(2-pyridylmethyl)methylamine] has been synthesized and structurally characterized by X-ray crystallography. The variable temperature-dependent susceptibility measurement (2-300 K) of 1 reveals a weak antiferromagnetic interaction between the manganese ions through the dichlorido bridging ligands, with J = -0.46 cm -1 , g = 1.99. The electrospray ionization mass spectrum of 1 in solution indicates that the dinu-

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Section: Uv Titrationmentioning

confidence: 99%

Synthesis, Crystal Structure, DNA Binding, and Hydrolytic Cleavage Activity of a Manganese(II) Complex

Qian

et al. 2010

Eur J Inorg Chem

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“…complex is 1.6 9 10 4 M -1 . This K b value is smaller than those reported for typical classical intercalators (EB-DNA, 3.3 9 10 5 M -1 in 50 mM Tris-HCl/1.0 M NaCl buffer, pH = 7.5) [32], but larger than that of the prototype complex [Ru(phen) 3 ] 2? (5.5 9 10 3 M -1 ) [33].…”

Section: Resultsmentioning

confidence: 55%

Luminescence properties of a di-ruthenium(II) complex with an intramolecular hydrogen bond modulated by DNA and copper(II) ion

Chen

Chao

et al. 2009

Transition Met Chem

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Modulation of the luminescence properties of a di-ruthenium(II) complex [(bpy) 2 Ru(BL)Ru(bpy) 2 ] 4? (bpy = 2,2 0 -bipyridine, BL = 2-hydroxyl-5-methyl-1,3-bis([1,10]phenanthroline-[5,6-d]imidazol-2-yl)benzene) by DNA and/or Cu 2? ion has been investigated. It is found that the ruthenium(II) complex can coordinate to the Cu 2? ion in both the absence and presence of DNA. Binding to DNA is through electrostatic interactions and the intramolecular hydrogen bond in the complex is located outside of the DNA. The binding constant is 1.6 9 10 4 M -1 . Moreover, it is demonstrated that DNA has the ability to enhance the luminescence intensities of both the di-ruthenium(II) complex and the tri-metallic system generated by chelating with Cu 2? . Conversely, Cu 2? ion can quench the luminescence of both the free ruthenium(II) complex and the DNA-bound ruthenium(II) complex.

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“…An aliquot containing 12.5 µM DNA was sequentially added at each step until saturation. Scatchard binding constants were calculated using modified Scatchard method (Strothkamp & Stothkamp 1994). Fluorescence spectroscopic experiments on the interaction of 1,4-DHP with the DNA-EtBr complex were carried out at room temperature in 5 mM Tris HCl; 50 mM NaCl at pH 7.4 or other buffer using a 1 cm cuvette (2ml).…”

Section: Fluorescence Spectroscopic Measurementsmentioning

confidence: 99%

Interaction of antimutagenic 1,4-dihydropyridine AV-153-Na with DNA and DNA-damaging molecules and its impact on DNA repair activity

Ļeonova

Rostoka

Sauvaigo³

et al. 2017

Preprint

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1,4-dihydropyridines (1,4-DHP) possess important biochemical and pharmacological properties, including antioxidant and antimutagenic activities. Interaction of some 1,4-DHP with DNA was recently reported. AV-153-Na, an antimutagenic and DNA-repair-enhancing compound appeared to be able to interact with DNA by intercalation.The aim of the current study was to characterize DNA's capacity for the binding of AV-153-Na, and using different approaches, to test intracellular distribution of the compound, to test the ability of the compound to scavenge peroxynitrite and hydroxyl radical and to assess the ability of the compound to modify the activity of DNA repair enzymes.The DNA binding activity of AV-153-Na was determined by means of fluorescence assay. Laser confocal scanning fluorescence microscopy demonstrated that within the cells AV-153-Na was found mostly in the cytoplasm; however, a stain in nucleolus was also detected. Binding to cytoplasmic structures might occur due to high affinity of the compound to protein, revealed by fluorescence spectroscopy titration and circular dichroism. Activation of DNA repair enzymes after binding to DNA appears to be the basis for the antimutagenic effects of AV-153-Na.

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Fluorescence Measurements of Ethidium Binding to DNA

Abstract: Quantitating the binding of ethidium bromide with DNA by monitoring the fluorescence of an ethidium-DNA solution.

Cited by 112 publications

References 3 publications

Synthesis, Crystal Structure, DNA Binding, and Hydrolytic Cleavage Activity of a Manganese(II) Complex

Synthesis, Crystal Structure, DNA Binding, and Hydrolytic Cleavage Activity of a Manganese(II) Complex

Luminescence properties of a di-ruthenium(II) complex with an intramolecular hydrogen bond modulated by DNA and copper(II) ion

Interaction of antimutagenic 1,4-dihydropyridine AV-153-Na with DNA and DNA-damaging molecules and its impact on DNA repair activity

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