Fluorescence Lifetime Techniques in Medical Applications

Marcu, Laura

doi:10.1007/s10439-011-0495-y

Cited by 212 publications

(205 citation statements)

References 131 publications

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“…Building on the pioneering single-point clinical measurements of autofluorescence lifetime, e.g. [9,10] and subsequent work recently reviewed in 2012 [11], clinical FLIM can combine lifetime contrast with morphological information to provide a direct comparison between different spatial regions -making it easier to spot differences with respect to "normal" tissue, e.g. for diagnostic screening and potentially enabling margins of diseased tissue to be identified.…”

Section: Introductionmentioning

confidence: 99%

A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue

Sparks

Warren

Guedes

et al. 2014

Journal of Biophotonics

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Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. Here we present progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ∼mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ∼8 seconds using an average excitation power of ∼0.5 mW at the specimen. To illustrate its potential for FLIM at higher acquisition rates, a higher power mode‐locked frequency doubled Ti:Sapphire laser was used to demonstrate FLIM of ex vivo mouse bowel at up to 2.5 Hz using 10 mW of average excitation power at the specimen. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)

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Section: Introductionmentioning

confidence: 99%

A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue

Sparks

Warren

Guedes

et al. 2014

Journal of Biophotonics

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“…In this instance, the sample is excited by a modulated light source. The fluorescence is modulated and phase-shifted from the original waveform based upon the lifetime of the fluorophore [34,40,54]. …”

Section: Fluorescent Lifetimementioning

confidence: 99%

Fluorescent labeling and modification of proteins

2013

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This review provides an outline for fluorescent labeling of proteins. Fluorescent assays are very diverse providing the most sensitive and robust methods for observing biological processes. Here, different types of labels and methods of attachment are discussed in combination with their fluorescent properties. The advantages and disadvantages of these different methods are highlighted, allowing the careful selection for different applications, ranging from ensemble spectroscopy assays through to single-molecule measurements.

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“…A variety of reviews for FLIM have now been documented. [1][2][3][4][5][6][7][8][9][10][11][12] Both endogenous and exogenous fluorophores are used as fluorescence probes for investigating intracellular environments. In the present review, representative exogenous fluorophores suitable for FLIM are described, giving a basis for selecting the fluorophore used for measuring the intracellular environment with FLIM.…”

Section: Introductionmentioning

confidence: 99%

Sensing of Intracellular Environments by Fluorescence Lifetime Imaging of Exogenous Fluorophores

Nakabayashi

Ohta

2015

ANAL. SCI.

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Fluorescence lifetime imaging (FLIM) has been recognized as a powerful microscopy technique to examine environments in living systems. The fluorescence lifetime does not depend on the photobleaching and optical conditions, which allows us to obtain quantitative information on intracellular environments by analyzing the fluorescence lifetime. A variety of exogenous fluorophores have been applied in FLIM measurements to examine cellular processes. Information on the correlation between the fluorescence lifetime and the physiological parameters is essential to elucidate the cellular environments from the fluorescence lifetime measurements of exogenous fluorophores. In this review, exogenous fluorophores used for lifetime-based sensing are summarized, with the expectation that it becomes a basis for selecting the fluorophore used to investigate the intracellular environment with FLIM. Experimental results of the intracellular sensing of pH, metal ions, oxygen, viscosity, and other physiological parameters on the basis of the FLIM measurements are described along with a brief explanation of the mechanism of the change in the fluorescence lifetime.

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Fluorescence Lifetime Techniques in Medical Applications

Cited by 212 publications

References 131 publications

A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue

A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue

Fluorescent labeling and modification of proteins

Sensing of Intracellular Environments by Fluorescence Lifetime Imaging of Exogenous Fluorophores

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