Fluorescence lifetime sorting reveals tunable enzyme interactions within cytoplasmic condensates
Leyla E. Fahim,
Joshua M. Marcus,
Noah D. Powell
et al.
Abstract:Ribonucleoprotein (RNP) condensates partition RNA and protein into multiple liquid phases. The multiphasic feature of condensate-enriched components creates experimental challenges for distinguishing membraneless condensate functions from the surrounding dilute phase. We combined fluorescence lifetime imaging microscopy (FLIM) with phasor plot filtering and segmentation to resolve condensates from the dilute phase. Condensate-specific lifetimes were used to track protein–protein interactions by measuring FLIM–… Show more
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