Fluorescence lifetime imaging to differentiate bound from unbound ICG-cRGD both<i>in vitro</i>and<i>in vivo</i>

Stegehuis, Paulien L.; Boonstra, Martin C.; Rooij, Karien E. de; Powolny, F.; Sinisi, Riccardo; Homulle, Harald; Bruschini, Claudio; Charbon, Edoardo; Velde, Cornelis J.H. van de; Lelieveldt, Boudewijn P. F.; Vahrmeijer, Alexander L.; Dijkstra, Jouke; Giessen, Martijn van de

doi:10.1117/12.2078644

Cited by 6 publications

(7 citation statements)

References 7 publications

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“…which target the α v β 3 integrin; the final goal was to explore the feasibility of surgical applications with exogenous NIR targeted fluorophores [25,55]. The system was capable of discriminating between healthy (muscle and tail) and cancerous tissues in a mouse with a glioblastoma mouse model, even though the lifetime difference was only about 50 ps (10% level in this case) between the lifetimes of the bound and unbound fluorophores as shown in Figure 3(a).…”

Section: Point-like Flimmentioning

confidence: 99%

Single-photon avalanche diode imagers in biophotonics: review and outlook

et al. 2019

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Single-photon avalanche diode (SPAD) arrays are solid-state detectors that offer imaging capabilities at the level of individual photons, with unparalleled photon counting and time-resolved performance. This fascinating technology has progressed at a very fast pace in the past 15 years, since its inception in standard CMOS technology in 2003. A host of architectures have been investigated, ranging from simpler implementations, based solely on off-chip data processing, to progressively “smarter” sensors including on-chip, or even pixel level, time-stamping and processing capabilities. As the technology has matured, a range of biophotonics applications have been explored, including (endoscopic) FLIM, (multibeam multiphoton) FLIM-FRET, SPIM-FCS, super-resolution microscopy, time-resolved Raman spectroscopy, NIROT and PET. We will review some representative sensors and their corresponding applications, including the most relevant challenges faced by chip designers and end-users. Finally, we will provide an outlook on the future of this fascinating technology.

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Section: Point-like Flimmentioning

confidence: 99%

Single-photon avalanche diode imagers in biophotonics: review and outlook

et al. 2019

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“…At least two dyes currently in the FDA approval pipeline, IRDye800CW and chlorotoxin-Cy5.5 (tumor paint), 6,23 allow specific targeting. Probes can also be designed to shift FL upon binding to disease-specific molecular expression, 18,24,25 allowing an "optical switch" property that indicates the presence or absence of a particular disease. As more of these dyes advance to the clinic, FLT imaging can offer enhanced specificity and sensitivity for various disease applications.…”

Section: Journal Of Biomedicalmentioning

confidence: 99%

“…15,16 ICG has also been evaluated as a targeted marker in preclinical models using fluorescence lifetime (FLT) to distinguish bound from unbound probes, for example, by conjugation with peptides targeting integrins. 17,18 However, these probes are still under preclinical evaluation and would need to go through the FDA approval pipeline before clinical translation, since they involve changes in chemical structure from ICG.…”

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confidence: 99%

Fluorescence lifetime-based contrast enhancement of indocyanine green-labeled tumors

et al. 2017

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Although the development of tumor-targeted fluorescent probes is a major area of investigation, it will be several years before these probes are realized for clinical use. Here, we report an approach that employs indocyanine-green (ICG), a clinically approved, nontargeted dye, in conjunction with fluorescence lifetime (FLT) detection to provide high accuracy for tumor-tissue identification in mouse models of subcutaneous human breast and brain tmors. The improved performance relies on the distinct FLTs of ICG within tumors versus tissue autofluorescence and is further aided by the well-known enhanced permeability and retention of ICG in tumors and the clearance of ICG from normal tissue several hours after intravenous injection. We demonstrate that FLT detection can provide more than 98% sensitivity and specificity, and a 10-fold reduction in error rates compared to intensity-based detection. Our studies suggest the significant potential of FLT-contrast for accurate tumor-tissue identification using ICG and other targeted probes under development, both for intraoperative imaging and for ex-vivo margin assessment of surgical specimens.

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“…The use of analog counters was also proposed to ensure large resolution at low cost in terms of fill factor [ 17 , 18 , 19 ]. Due to the lack of a TDC though, these methods require a precise gate and significant algorithmic complexity [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Photon-Counting Arrays for Time-Resolved Imaging

Antolović

Burri

Hoebe

et al. 2016

Sensors

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Abstract:The paper presents a camera comprising 512ˆ128 pixels capable of single-photon detection and gating with a maximum frame rate of 156 kfps. The photon capture is performed through a gated single-photon avalanche diode that generates a digital pulse upon photon detection and through a digital one-bit counter. Gray levels are obtained through multiple counting and accumulation, while time-resolved imaging is achieved through a 4-ns gating window controlled with subnanosecond accuracy by a field-programmable gate array. The sensor, which is equipped with microlenses to enhance its effective fill factor, was electro-optically characterized in terms of sensitivity and uniformity. Several examples of capture of fast events are shown to demonstrate the suitability of the approach.

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Fluorescence lifetime imaging to differentiate bound from unbound ICG-cRGD bothin vitroandin vivo

Cited by 6 publications

References 7 publications

Single-photon avalanche diode imagers in biophotonics: review and outlook

Single-photon avalanche diode imagers in biophotonics: review and outlook

Fluorescence lifetime-based contrast enhancement of indocyanine green-labeled tumors

Photon-Counting Arrays for Time-Resolved Imaging

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