Fluorescence lifetime imaging: an application to the detection of skin tumors

Cubeddu, Rinaldo; Pifferi, Antonio; Taroni, Paola; Torricelli, Alessandro; Valentini, Gianluca; Sorbellini, Elisabetta

doi:10.1109/2944.796312

Cited by 61 publications

(41 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cubeddu et al [15] developed a portable system based on fluorescence lifetime imaging to detect human skin tumors. After ALA was applied on tumor tissues for a period of time, a 337-nm pulsed nitrogen laser was used to excite the accumulated protoporphyrin IX (PpIX).…”

Section: Introductionmentioning

confidence: 99%

Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues

et al. 2005

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues

et al. 2005

View full text Add to dashboard Cite

show abstract

“…FLIM has already been applied to studies of tissue constituents, 6 cell cultures, 7 and the human skin. 8,9 FLIM can be performed either in the frequency domain, for which a high-frequency modulated laser beam excites the sample and the fluorescence lifetime is determined from the demodulation and phase shift of the fluorescence signal, 10 or in the time domain, for which the fluorescence decay is directly measured after pulsed laser excitation. 11 With either approach, functional information may be derived from the fluorescence lifetime by means of its dependence on the radiative decay rate k r and the nonradiative decay rate k nr of the fluorophore, where ϭ 1͑͞k r ϩ k nr ͒.…”

Section: Introductionmentioning

confidence: 99%

Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles

Siegel¹,

Elson²,

Webb³

et al. 2003

Appl. Opt.

102

View full text Add to dashboard Cite

We have applied fluorescence lifetime imaging ͑FLIM͒ to the autofluorescence of different kinds of biological tissue in vitro, including animal tissue sections and knee joints as well as human teeth, obtaining two-dimensional maps with functional contrast. We find that fluorescence decay profiles of biological tissue are well described by the stretched exponential function ͑StrEF͒, which can represent the complex nature of tissue. The StrEF yields a continuous distribution of fluorescence lifetimes, which can be extracted with an inverse Laplace transformation, and additional information is provided by the width of the distribution. Our experimental results from FLIM microscopy in combination with the StrEF analysis indicate that this technique is ready for clinical deployment, including portability that is through the use of a compact picosecond diode laser as the excitation source. The results obtained with our FLIM endoscope successfully demonstrated the viability of this modality, though they need further optimization. We expect a custom-designed endoscope with optimized illumination and detection efficiencies to provide significantly improved performance.

show abstract

“…Moreover, Cubeddu and co-workers [26,27] used fluorescence lifetime imaging (FLIM) to study tumors in human skin. The instrumentation consisted of a dye laser pulsing at 405 nm at a pulse width of less than 1 ns and a gated imaging system.…”

Section: Time-resolved Fluorescence Measurements On Tissuementioning

confidence: 99%

In vivo time-resolved autofluorescence measurements to test for glycation of human skin

Blackwell¹,

Katika²,

Pilon³

et al. 2008

J. Biomed. Opt.

View full text Add to dashboard Cite

This paper presents an evaluation of time-resolved fluorescence measurements on human skin for screening type 2 diabetes. In-vivo human skin was excited with a pulse diode at 375 nm and pulse width of 700 ps. Fluorescence decays were recorded at four different emission wavelengths 442, 460, 478 and 496 nm. Experiments were performed on various locations including the palms, arms, legs, and cheeks of a healthy Caucasian subject to test single-subject variability. The fluorescence decays obtained were modeled using a three-exponential decay. The variations in the lifetimes and amplitudes from one location to another were minimal except on the cheek. The study compares the fluorescent decays of 38 diabetic subjects and 37 nondiabetic subjects, with different skin complexions and of ages ranging from 6 to 85 years. The average lifetimes for nondiabetic subjects were 0.5, 2.6 and 9.2 ns with fractional amplitudes of 0.78, 0.18 and 0.03, respectively. The effects of average hemoglobin A1c (HbA1c) from the previous four years and diabetes duration were evaluated. While no significant differences between the fluorescence lifetimes of nondiabetic and diabetic subjects were observed, two of the fractional amplitudes were statistically different. Additionally, none of the six fluorescence parameters correlated with diabetes duration or HbA1c.One of the lifetimes as well as two of the fractional amplitudes differed between diabetic subjects with foot ulcers and nondiabetic subjects.

show abstract

Fluorescence lifetime imaging: an application to the detection of skin tumors

Cited by 61 publications

References 37 publications

Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues

Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues

Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles

In vivo time-resolved autofluorescence measurements to test for glycation of human skin

Contact Info

Product

Resources

About