Fluorescence Lifetime and UV‐Vis Spectroscopy to Evaluate the Interactions Between Quercetin and Its Yeast Microcapsule

Pham-Hoang, Bao-Ngoc; Winckler, Pascale; Waché, Yves

doi:10.1002/biot.201700389

Cited by 14 publications

(12 citation statements)

References 30 publications

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“…The presence of catechins was further proved by the observation of characteristic peaks at about 270 nm in the UV–vis absorption spectrum (Figure S8). The new emerging peak at about 375 nm might be related to the oxidation of TPs. , The analysis above suggested that the GNPs were successfully prepared by the separated processes and the whole program did not involve harsh chemicals and extra energy inputs.…”

Section: Results and Discussionmentioning

confidence: 98%

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Green Nanoparticle Scavengers against Oxidative Stress

Yang

Zhang

Xiang

et al. 2021

ACS Appl. Mater. Interfaces

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The usage of exogenous antioxidant materials to relieve oxidative stress offers an important strategy for the therapy of oxidative stress-induced injuries. However, the fabrication processes toward the antioxidant materials usually require the involvement of extra metal ions and organic agents, as well as sophisticated purification steps, which might cause tremendous environmental stress and induce unpredictable side effects in vivo. To address these issues, herein, we proposed a novel strategy to fabricate green nanoparticles for efficiently modulating oxidative stress, which was facilely prepared from tea polyphenol extracts (originated from green tea) via a green enzymatic polymerization-based chemistry method. The resulting nanoparticles possessed a uniform spherical morphology and good stability in water and biomedium and demonstrated excellent radical scavenging properties. These nanoparticle scavengers could effectively prevent intracellular oxidative damage, accelerate wound recovery, and protect the kidneys from reactive oxygen species damaging in the acute kidney injury model. We hope this work will inspire the further development of more types of green nanoparticles for antioxidant therapies via similar synthetic strategies using green biomass materials.

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Section: Results and Discussionmentioning

confidence: 98%

“…The new emerging peak at about 375 nm might be related to the oxidation of TPs. 47,48 The analysis above suggested that the GNPs were successfully prepared by the separated processes and the whole program did not involve harsh chemicals and extra energy inputs.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Green Nanoparticle Scavengers against Oxidative Stress

Yang

Zhang

Xiang

et al. 2021

ACS Appl. Mater. Interfaces

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“…In order to further observe the variations in quercetin distribution under different spinning flow rates, fluorescence microscopy was used to analyze the cross section of the fibers. It is known that quercetin presents a strong fluorescence display, − whereas the regenerated cellulose does not, allowing the observation of quercetin distribution in fibers using a fluorescence microscope. However, FT-IR and XRD analyses revealed that the chemical structure and crystalline state of quercetin regenerated from [Emim]Ac had undergone changes, resulting in the loss of its fluorescence properties (Figure a).…”

Section: Resultsmentioning

confidence: 99%

Preparation of Antibacterial Biobased Fibers by Triaxial Microfluidic Spinning Technology Using Ionic Liquids as the Solvents

Zhao,

Wang,

Yuan

et al. 2024

ACS Appl. Mater. Interfaces

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Bacterial infections have become a serious threat to public health. The utilization of antibacterial textiles offers an effective way to combat bacterial infections at the source, instead of relying solely on antibiotic consumption. Herein, efficient and durable antibacterial fibers based on quercetin and cellulose were prepared by a triaxial microfluidic spinning technology using ionic liquids (ILs) as the solvents. It was indicated that the structure and properties of the antibacterial fibers were affected by the type of IL and the flow rates during the triaxial microfluidic spinning process. Quercetin regenerated from [Emim]Ac underwent structural transformation and obtained an increased water solubility, while quercetin regenerated from [Emim]DEP remained unchanged, which was proven by FI-IR, XRD, and UV analyses. Furthermore, antibacterial fibers regenerated from [Emim]Ac exhibited the highest antibacterial activity of 96.9% against S. aureus, achieved by reducing the inner-to-outer flow rate ratio to 0 and concentrating quercetin at the center of fibers. On the other hand, when [Emim]DEP was used as the solvent, balancing the inner-to-outer flow rate ratio to concentrate quercetin in the middle layer of the fiber was optimal for achieving the best antibacterial activity of 93.3% because it promised both the higher encapsulation efficiency and release rate. Computational fluid dynamics (CFD) mathematically predicted the solvent exchange process during triaxial spinning, explaining the influence of IL types and flow rates on quercetin distribution and encapsulation efficiency. It was indicated that optimizing the distribution of antibacterial agents within the fibers can fully unleash its antibacterial potential while preserving the mechanical properties of the fiber. Therefore, the proposed simple triaxial spinning strategy provides valuable insights into the design of biomedical materials.

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“…The cell-based carriers selected in this study include yeast cells (YC), including alive yeast cell carriers (aYC) and deactivated yeast cell carriers (dYC), and yeast cell wall particles (YCWP). Yeast microcarrier and its derivatives are widely accepted encapsulation matrixes for food applications based on their “generally recognized as safe” (GRAS) status. − YC and YCWP possess high encapsulation yield for polyphenolic compounds such as curcumin and significantly enhance physicochemical stability as demonstrated in the literature and our recent studies. , Moreover, fungal–bacterial binding interactions are found ubiquitously in the environment and particularly in biofilms. − Therefore, based on this background knowledge, this study evaluated the role of these diverse cell carrier compositions derived from yeast cells for the encapsulation of curcumin, binding of cell carrier compositions to biofilms, their efficacy in inactivation of bacteria in the selected biofilm model, and influence on the structure of the biofilm after treatment with the selected compositions. Listeria innocua (ATCC 33090) was selected as a nonpathogenic surrogate for the foodborne pathogen Listeria monocytogenes, which is a serious concern for the food industry based on its ubiquitous presence in the environment and biofilm-forming ability .…”

Section: Introductionmentioning

confidence: 99%

Targeted Photodynamic Treatment of Bacterial Biofilms Using Curcumin Encapsulated in Cells and Cell Wall Particles

Dou

Huang

Nitin

2020

ACS Appl. Bio Mater.

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Efficient inactivation and removal of pathogenic biofilms in food and biomedical environments remain a significant challenge for food safety applications and medical facilities. This research aims to develop food-grade microcarriers for the targeted delivery of a photosensitizer, curcumin, and photodynamic inactivation of a model pathogenic bacterial biofilm. The microcarriers evaluated in this study include alive yeast cell carriers, deactivated yeast cell carriers, and yeast cell wall particles. The microcarriers were evaluated based on the encapsulation yield of a model photosensitizer (curcumin), binding of the microcarriers to biofilms, and inactivation of the bacteria in the biofilms. The results illustrate that the combination of binding affinity, encapsulation yield, and the intracellular composition of the microcarriers influenced the overall inactivation of bacteria in the biofilms. All of the selected compositions achieved more than 93% inactivation of the bacteria in the biofilm using the photodynamic treatment, and the yeast cell wall particles with curcumin achieved over 99% inactivation of the bacteria in the biofilm matrix. In addition, all of the selected compositions demonstrated significant potential to remove the biofilm from the plastic surface, suggesting the role of binding affinity of the microcarriers in removal of the biofilm from surfaces. Overall, this study developed biomaterial formulations for targeted photodynamic inactivation and potential removal of biofilms.

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Fluorescence Lifetime and UV‐Vis Spectroscopy to Evaluate the Interactions Between Quercetin and Its Yeast Microcapsule

Cited by 14 publications

References 30 publications

Green Nanoparticle Scavengers against Oxidative Stress

Green Nanoparticle Scavengers against Oxidative Stress

Preparation of Antibacterial Biobased Fibers by Triaxial Microfluidic Spinning Technology Using Ionic Liquids as the Solvents

Targeted Photodynamic Treatment of Bacterial Biofilms Using Curcumin Encapsulated in Cells and Cell Wall Particles

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