Fluorescence investigations to classify malignant laryngeal lesions in vivo

Abstract

There are differences in the in vivo tissue fluorescence between tissue with different gradings of dysplasia and carcinoma.

Optical and molecular techniques to identify tumor margins within the larynx

“…This difference in autofluorescence can be detected by inspecting the mucosa with a specialized endoscope (see Figure 2); or, alternatively, the difference in fluorescence can be quantified by splitting each reflected light into its constituent wavelengths and plotting intensity at each wavelength. 10 Several studies have demonstrated that autofluorescence endoscopy and spectroscopy are better at identifying invasive carcinoma when compared with microlaryngoscopy alone, 5,10-15 and many head and neck oncology centers use autofluorescence as an adjunct to microlaryngoscopic examination. There are, however, several limitations to autofluorescence examination.…”

“…[19][20][21] Furthermore, ALA-induced fluorescence may be better than autofluorescence at distinguishing recurrent cancer from scar tissue following laser surgery. 21 Recently, however, Rydell et al 10 reported that the addition of ALA-induced fluorescence spectroscopy to autofluorescence examination did not further improve diagnostic accuracy. 10 As would be expected, ALA-induced fluorescence examination shares many of the limitations of autofluorescence described earlier.…”

“…21 Recently, however, Rydell et al 10 reported that the addition of ALA-induced fluorescence spectroscopy to autofluorescence examination did not further improve diagnostic accuracy. 10 As would be expected, ALA-induced fluorescence examination shares many of the limitations of autofluorescence described earlier. Of particular importance is that neither technique demonstrates histologic detail, which is required to differentiate between grades of dysplasia, and invasive carcinoma.…”

Role of Advanced Laryngeal Imaging in Glottic Cancer

“…51,57,58 However, the addition of ALA-induced fluorescence to AF does not improve diagnostic accuracy. 59 ALA-induced fluorescence may also be better than AF in differentiating recurrent cancer from scar tissue after laser surgery. 51 Like AF, ALA-induced fluorescence cannot be used to determine histologic detail and thus cannot characterize grade of dysplasia or identify invasive carcinoma.…”

Fluorescence diagnostics of skin tumors using 5-aminolevulinic acid and its methyl ester

“…ir 100 proc. (25). Geri rezultatai aprašomi ir neurochirurgų: FD padeda tiksliau nustatyti galvos smegenų naviko ribas ir atlikti radikalesnę operaciją (26,27).…”