Fluorescence induction in whole leaves: Differentiation between the two leaf sides and adaptation to different light regimes

Schreiber, Ulrich; Fink, Ronald Peter; Vidaver, William

doi:10.1007/bf00391909

Cited by 83 publications

(40 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could occur if the number of antenna molecules were greater or if energy distribution between the reaction centers and photosystems favored an increased density of excited PSII pigments (17). As cucumber leaves expanded, the time to P increased as the rate of the rise to P declined, indicating changes in PSII acceptors (7,15). The maximum changes in the time and rise to P coincided with the narrowing of the M peak in leaves 1 and 2 ( Fig.…”

Section: Discussionmentioning

confidence: 98%

Patterns of Chlorophyll Fluorescence Kinetics in Relation to Growth and Expansion in Cucumber Leaves

Croxdale¹,

Omasa²

1990

Plant Physiol.

View full text Add to dashboard Cite

MATERIALS AND METHODSPhotochemical development was studied in developing cucumber (Cucumis sativus L. cv Hokushin) leaves to determine if the spatial pattern coincided with relative growth rates of expanding leaves, intercalary cell division, or position relative to the vascular tissue. Both leaf surfaces undergo a series of similar changes in chlorophyll a fluorescence kinetics, but the upper surface more quickly achieved the characteristic response. Imaging of fluorescence showed an individual developing leaf has four regions differing in kinetics, but these regions do not coincide with areas of increasing relative growth rate. Two of these regions lie at the leaf edge and their divergent kinetics may be related to structural and physiological features present at this position. A third area with different kinetics, in the basal region of the leaf, is spatially consistent with primordial regions that are clonal during development. The correspondence between areas of clonal growth and specific fluorescence kinetics indicates that cells of common ancestry show functional uniformity. No evidence was found that the proximity of the vascular tissue influenced development of photochemical function.The attainment of photosynthetic competency in angiosperm leaves is regulated by light and by developmental factors. Unlike monocots such as wheat (2), cucumber leaves are not photochemically competent when first exposed to light, but acquire competency over an extended time period, 6 d or more in the light (5). Within these leaves photochemical activity is not homogeneous, but develops basipetally as shown by Chl a fluorescence transients. These data indicate that the primary regulation ofthe development ofphotochemical activity lies with developmental factors, not light (5).Although the attainment of characteristic fluorescence transients progresses in a basipetal direction, mirroring the general direction ofexpansion and growth ofthe leaf(5), the influence of developmental factors from cells and tissues within the leaves has not been studied. The objective of the present research was to determine ifthe spatial pattern of photochemical development coincided with leaf expansion and relative growth rates, intercalary cell division, or position relative to the vascular tissue. With the availability of dynamic imaging of Chl a fluorescence (10), a precise, nondestructive means of studying these influences exists.'Supported by a National Science Foundation grant (JGC). Plant Materials and Leaf Area MeasurementsCucumber (Cucumis sativus L. cv Hokushin) plants were grown as previously described (10) for up to 5 weeks. For fluorescence and gas exchange measurements, plants were brought from the controlled-environment greenhouse to the laboratory and allowed to acclimate for about 2 h. Plants were dark-incubated for at least 30 min before fluorescence induction. No appreciable differences in induction kinetics were observed with longer dark periods. Leaves monitored for fluorescence and assimilation were intact and remain...

show abstract

Section: Discussionmentioning

confidence: 98%

Patterns of Chlorophyll Fluorescence Kinetics in Relation to Growth and Expansion in Cucumber Leaves

Croxdale¹,

Omasa²

1990

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Richard et al [1983] detected an increase in the terminal level of fluorescence in plants following the foliar application of herbicides, which inhibit photosynthetic electron transport. Schreiber et al [1977] found that ozone-induced injury could be detected by the fluorescence assay at least 20 hours before any visible signs of leaf injury. It is also known that the F684/F735 ratio, reflecting PSII and PSI activities, can be used as a tool to assess performance of the photosynthetic apparatus [Agati et al, 1995;Lichtenthaler et al, 1996].…”

Section: Introductionmentioning

confidence: 99%

“…Under optimal conditions, most of the light energy absorbed by chlorophyll is dissipated via chemical conversion, with a small proportion being emitted through heat and fluorescence. When photosynthetic electron transport is blocked, an increased proportion of the absorbed excitation energy is reemitted as fluorescence [Schreiber et al, 1977;Agati et al, 1995]. 460 The analysis of changes in chlorophyll fluorescence (ChlF) offers possibilities not only to determine the site of electron transport inhibition by herbicides, but also to evaluate injuries from herbicides.…”

Section: Introductionmentioning

confidence: 99%

Tracking photosynthetic injury of Paraquat-treated crop using chlorophyll fluorescence from hyperspectral data

Liu

Zhao

Guan

2013

European Journal of Remote Sensing

View full text Add to dashboard Cite

Three diurnal experiments were conducted on winter wheat to investigate how solarinduced chlorophyll fluorescence (SIF) responded to Paraquat treatments (a PSI herbicide). First, a slight increase in spectral reflectance in the red region and an obvious decrease in reflectance in the NIR regions was observed due to crop injury from Paraquat. Secondly, a significant increase in SIF at 688 nm, but a significant decrease at 760 nm was observed. The opposite response trends of SIF confirm the mechanism of PSI herbicides in photosynthetic injury. Finally, the sensitivity of SIF signals were compared to other vegetation indices, and the Fr688/760 ratio turned out to be a sensitive tool to rapidly detect herbicide injury.

show abstract

“…4 and 5) were typical of 'sun' leaves, whereas the under sides of leaves (Fig. 5) gave responses characteristic of 'shade' leaves (21). These differences are evident in the 1-s transients in which the curves measured on upper leaf surfaces show a longer intermediate step (I to D) and a slower rise to the peak (D to P) than those measured on the lower leafsurface.…”

Section: Resultsmentioning

confidence: 90%

Photosynthetic Responses to Irradiance by the Grey Mangrove, Avicennia marina, Grown under Different Light Regimes

Ball¹,

Critchley²

1982

Plant Physiol.

View full text Add to dashboard Cite

Photosynthetic responses to irradiance by the grey mangrove, Avcennia marina (Forstk.) Vierh var. ustraluia (Walp.) Moldenke, were studied using seedlings grown under natural understory shade and exposed conditions as well as in the laboratory under high and low lght regimes, ie. 100% and 6% sunlight, respectively. Leaves in exposed locations were subjected to dayUght quantum flux densities greater than 1,000 microeinsteins per square meter per second from 0900 to 1700 hours, whereas those in understory shade experienced only 30 to 120 microeinsteins per square meter per second, interrupted for brief periods by sunflecks rangin in quantum flux density from 800 to 1,500 microeinsteins per square meter per second. The low lght regime was similar in lght intensity to that of the understory environment, but lacked sunflecks.Leaves from the understory environment showed several properties of 'shade' leaves; ie. they contained more chlorophyll on both a leaf area and fresh weight basis but had a lower specific weight and greater area than exposed leaves, and were enriched in chlorophyll b relative to a. However, there were no significant differences in either the gas exchange or leaf chlorophyll fluorescence characteristics of the two populations, both being typical of 'sun' leaves. Leaves grown in the laboratory under low and high light regimes had similar properties. However, light saturated assimilation rates in the leaves from the low light treatment were 20% less and became light saturated at a lower quantum flux density than those of leaves grown under the high light regime. The ecological significance of these results is discussed.

show abstract

Fluorescence induction in whole leaves: Differentiation between the two leaf sides and adaptation to different light regimes

Cited by 83 publications

References 18 publications

Patterns of Chlorophyll Fluorescence Kinetics in Relation to Growth and Expansion in Cucumber Leaves

Patterns of Chlorophyll Fluorescence Kinetics in Relation to Growth and Expansion in Cucumber Leaves

Tracking photosynthetic injury of Paraquat-treated crop using chlorophyll fluorescence from hyperspectral data

Photosynthetic Responses to Irradiance by the Grey Mangrove, Avicennia marina, Grown under Different Light Regimes

Contact Info

Product

Resources

About