Fluorescence in situ hybridization in diagnostic cytology

Halling, Kevin C.; Kipp, Benjamin R.

doi:10.1016/j.humpath.2007.04.015

Cited by 98 publications

(72 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study showed similar rates which is consistent with previous studies. Fluorescence in situ hybridization (FISH) has been increasingly used to facilitate the identification of neoplastic cells in cytologic specimens [27] . In one study, 20% of patients with cholangiocarcinoma missed by conventional cytology were identified by FISH without affecting the specificity [28] .…”

Section: Discussionmentioning

confidence: 99%

Differentiation of malignant and benign proximal bile duct strictures: The diagnostic dilemma

Kloek¹,

Delden²,

Erdogan³

et al. 2008

WJG

View full text Add to dashboard Cite

AIM:To identify the criteria for the differentiation of hilar cholangiocarcinoma (HCCA) from benign strictures. METHODS: A total of 68 patients underwent resection of lesions suspicious for HCCA between 1998 and 2006. The results of laboratory investigations, imaging studies and brush cytology were collected. These findings were analyzed to obtain the final diagnosis. RESULTS: Histological examination of the resected specimens confirmed HCCA in 58 patients (85%, group Ⅰ) whereas 10 patients (15%, group Ⅱ) were diagnosed to have benign strictures. The most common presenting symptom was obstructive jaundice in 77% patients (79% group Ⅰ vs 60% group Ⅱ, P = 0.23). Laboratory findings showed greater elevation of transaminase levels in group Ⅰ compared to group Ⅱ. The various imaging modalities showed vascular involvement exclusively in the malignant group (36%, P < 0.05). Brush cytology was positive for malignant cells in only 50% patients in group Ⅰ whereas none in group Ⅱ showed malignant cells. CONCLUSION: Despite improvements in imaging techniques, 10 patients (15%) with a presumptive diagnosis of HCCA were ultimately found to have benign strictures. Except for vascular involvement which was associated significantly with malignancy, there were no conclusive features of malignancy on regular imaging modalities. This uncertainty should be taken into account when patients with a suspicious lesion at the liver hilum are considered for resection.

show abstract

Section: Discussionmentioning

confidence: 99%

Differentiation of malignant and benign proximal bile duct strictures: The diagnostic dilemma

Kloek¹,

Delden²,

Erdogan³

et al. 2008

WJG

View full text Add to dashboard Cite

show abstract

“…These findings are similar to results described by other researchers on biliary brushings and washings associated with biliary strictures. [9][10][11][12][13][14] Our patient follow-up studies helped us to look at the true sensitivity and specificity of UFISH as an adjuvant test to FNA. Of the 30 cases with FNA results that were suspicious, reactive, or negative, we feel we were able to definitively classify the condition of 7 patients as benign and of 9 as malignant, on the basis of our hospital medical records.…”

Section: Commentmentioning

confidence: 99%

“…They discontinued the use of DIA and performed routine cytology and UFISH for all patients being evaluated for pancreatobiliary malignancies. Other studies [12][13][14] have reported the utility and value of UFISH primarily in biliary brushings. Levy et al 15 published results of EUS-FNA cytology with DIA and UFISH of 19 patients with pancreatic masses, combined with 23 other patients with lymphadenopathy and esophageal, gastric, or thyroid masses.…”

mentioning

confidence: 99%

The Utility of UroVysion Fluorescence In Situ Hybridization in Pancreatic Fine-Needle Aspiration Samples Directed and Obtained by Endoscopic Ultrasonography

Henkes

Patel

Rosenkranz

et al. 2013

Archives of Pathology &Amp; Laboratory Medicine

View full text Add to dashboard Cite

N Context.-The diagnosis of pancreatic adenocarcinoma can be challenging for the pathologist. Endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA) can be used to obtain samples of pancreatic masses. UroVysion fluorescence in situ hybridization (UFISH) has been reported to increase the sensitivity and to be very specific for the diagnosis of adenocarcinoma when combined with cytology in the diagnosis of biliary brushings and washings.Objectives.-To determine the sensitivity and specificity of UFISH on tissues obtained from pancreatic lesions suggestive of adenocarcinoma obtained by EUS-FNA, compared against fine-needle aspiration (FNA) results. Additionally, to use patient follow-up data to evaluate UFISH results in FNA samples that showed significant atypia but did not meet the criteria for malignancy.Design.-Sixty consecutive cases of pancreatic EUS-FNA from our institution submitted for UFISH testing.Results.-Polysomic UFISH has a sensitivity of 93% and a specificity of 100% when compared against FNA results. Follow-up studies showed that adding UFISH to FNA increased the sensitivity for patients with true-positive results from 83% to 94% and increased specificity from 85% to 100%. For 7 patients with suspicious FNA results who had sufficient follow-up, UFISH was 100% sensitive and 100% specific.Conclusions.-UFISH can be used to confirm the diagnosis of malignancy in pancreatic adenocarcinoma. Because of the high specificity, polysomic UFISH may help establish a diagnosis of malignancy when the FNA features are suggestive of, but not conclusive for, malignancy. The most common cause for a false-negative UFISH result was insufficient numbers of malignant cells. (Arch Pathol Lab Med. 2013;137:64-71; doi: 10.5858/ arpa.2011-0241-OA) P ancreatic cancer is the fourth leading cause of cancerrelated death in the United States. In 2010, it was estimated that 6% of cancer deaths in males and 7% of cancer deaths in females were due to pancreatic cancer. The 5-year survival rate for all stages of pancreatic cancer is the lowest of all the major cancer sites.

show abstract

“…3 Therefore, this technique has become a helpful tool in cytology to better distinguish reactive from neoplastic lesions. 4 The multitarget FISH assay (UroVysion; Abbott Molecular Inc, Des Plaines, Ill) was developed to detect polysomies of the chromosomes 3, 7, and 17, and deletion of the gene locus 9p21. 5 This assay has been used successfully to improve the diagnostic yield of urinary cytology.…”

mentioning

confidence: 99%

Evaluation of chromosomal aberrations in patients with benign conditions and reactive changes in urinary cytology

Tapia¹,

Glatz²,

Obermann³

et al. 2011

Cancer Cytopathology

View full text Add to dashboard Cite

BACKGROUND:Fluorescence in situ hybridization (FISH) is routinely used to help clarify atypical urinary cytology. However, to the authors' knowledge, little is known regarding the frequency of chromosomal aberrations in non‐neoplastic conditions that could potentially lead to false‐positive FISH results. The objective of the current study was to evaluate the frequency of chromosomal aberrations in benign cells of the urinary tract using the UroVysion FISH test.METHODS:The authors analyzed 77 Papanicolaou‐stained benign urine cytology specimens with reactive epithelial atypia using a FISH assay detecting the chromosomes 3, 7, and 17 and the gene locus 9p21. A positive test result was defined as an increased copy number of at least 2 chromosomes in ≥ 4 of 25 cells, or > 10 cells with a tetraploid or octaploid pattern, or homozygous or heterozygous deletion of 9p21 (≥ 12cells).RESULTS:FISH was positive in 27 of 77 bladder washings (35.1%) including 25 of 65 bladder washings (40.5%) and 2 of 15 voided urines (13.5%) from patients with irritative bladder (15 of 36 patients), a history of radiotherapy (7 of 12 patients), nonspecific cystitis (3 of 11 patients), hematuria (3 of 8 patients), and lithiasis (1 of 4 patients) . In 7 of 27 FISH‐positive urothelial specimens, the positivity was solely due a polyploid pattern (tetraploid/octaploid pattern) in > 10 of the cells.CONCLUSIONS:Chromosomal aberrations can occur in reactive urothelial cells, with a tetraploid pattern being the most common. Even an aneuploid pattern of FISH signals does not always prove malignancy because it rarely occurs in reactive urothelial cells. Correlation of FISH results with cytomorphology and patient history is crucial to avoid false‐positive diagnoses. Cancer (Cancer Cytopathol) 2011;. © 2011 American Cancer Society.

show abstract

Fluorescence in situ hybridization in diagnostic cytology

Cited by 98 publications

References 58 publications

Differentiation of malignant and benign proximal bile duct strictures: The diagnostic dilemma

Differentiation of malignant and benign proximal bile duct strictures: The diagnostic dilemma

The Utility of UroVysion Fluorescence In Situ Hybridization in Pancreatic Fine-Needle Aspiration Samples Directed and Obtained by Endoscopic Ultrasonography

Evaluation of chromosomal aberrations in patients with benign conditions and reactive changes in urinary cytology

Contact Info

Product

Resources

About