Fluorescence in situ hybridization for the identification of Treponema pallidum in tissue sections

Petrich, Annett; Rojas, Pablo-David; Schulze, Julia; Loddenkemper, Christoph; Giacani, Lorenzo; Schneider, Thomas; Hertel, Moritz; Kikhney, Judith; Moter, Annette

doi:10.1016/j.ijmm.2015.08.022

Cited by 17 publications

(10 citation statements)

References 43 publications

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“…This is in particular important for common contaminants from resident flora (e.g., skin flora like CNS or Propionibacterium spp. ). FISH can demonstrate the localization of the bacteria and differentiate between invasive (deep tissue) or superficial infections and identify predilection sites on implants, which may be useful for understanding likely sites to examine devices for bacteria . Regarding mixed infections, FISH is able to assess the abundance of different species and identify the key species or invasive population. FISH can differentiate between single bacteria and microcolonies or extensive biofilms (Figs and ). Together with culture and NA amplification techniques, this has potential for a ‘staging’ of biofilm infections in the future and the development of specific therapeutic strategies for the different stages. PNA FISH is becoming more widespread in diagnostic labs with the advent of validated kits designed for high‐throughput examination following blood culture.…”

Section: Diagnostic Methodsmentioning

confidence: 99%

“…This is in particular important for common contaminants from resident flora (e.g., skin flora like CNS or Propionibacterium spp. useful for understanding likely sites to examine devices for bacteria (83,84). • Regarding mixed infections, FISH is able to assess the abundance of different species and identify the key species or invasive population.…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

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Microbiological diagnosis of device‐related biofilm infections

Larsen

Lorenzen

et al. 2017

APMIS

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Medical device-related infections cause undue patient distress, increased morbidity and mortality and pose a huge financial burden on healthcare services. The pathogens are frequently distributed heterogeneously in biofilms, which can persist without being effectively cleared by host immune defenses and antibiotic therapy. At present, there is no 'gold standard' available to reveal the presence of device-related biofilm infections. However, adequate sample collection and logistics, standardised diagnostic methods, and interpretation of results by experienced personnel are important steps in efficient diagnosis and treatment of these infections. The focus of this mini review is on prosthethic joint and cardiovascular implantable device infections, which exemplify permanent devices that are placed in a sterile body site. These device-related infections represent some of the most challenging in terms of both diagnosis and treatment.

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Section: Diagnostic Methodsmentioning

confidence: 99%

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Microbiological diagnosis of device‐related biofilm infections

Larsen

Lorenzen

et al. 2017

APMIS

Self Cite

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“…However, major limitations of realtime qPCR include the inability to differentiate between live and dead organisms and the difficulty in efficient DNA extraction from complex tissues such as lymph nodes. Fluorescence in situ hybridization (FISH) allows for efficient recognition of T. pallidum in intact human and rabbit tissue samples [104] using a species-specific probe that recognizes 16 s rRNA of T. pallidum . FISH detection of T. pallidum would allow for additional information in experimental syphilis investigations regarding T. pallidum localization within tissue samples.…”

Section: Notable Developments In Methodologies Pertaining To Syphimentioning

confidence: 99%

Vaccine development for syphilis

Lithgow

Cameron

2016

Expert Review of Vaccines

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Introduction Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, continues to be a globally prevalent disease despite remaining susceptible to penicillin treatment. Syphilis vaccine development is a viable preventative approach that will serve to complement public health-oriented syphilis prevention, screening and treatment initiatives to deliver a two-pronged approach to stemming disease spread worldwide. Areas covered This article provides an overview of the need for development of a syphilis vaccine, summarizes significant information that has been garnered from prior syphilis vaccine studies, discusses the critical aspects of infection that would have to be targeted by a syphilis vaccine, and presents the current understanding within the field of the correlates of protection needed to be achieved through vaccination. Expert commentary Syphilis vaccine development should be considered a priority by industry, regulatory and funding agencies, and should be appropriately promoted and supported.

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“…; Chahorm and Prakitchaiwattana ) or a stable state, as occurs in Mycobacterium leprae and Treponema pallidum (Petricha et al . ; Ruiz‐Fuentesa et al . ; Gogarten et al .…”

Section: Introductionmentioning

confidence: 99%

“…These may not yet have been identified because they exhibit few indicative diagnostic characteristics through culture morphology (Kom arek et al 2014;Sciuto and Moro 2015), attenuated growth capacity or unclear growth requirements in culture, making them difficult to identify by traditional methodology (Youssef et al 2014;Sciuto and Moro 2015). In addition, they may not be able to be cultivated, thus known as viable, but not cultivable, which is either a transitory state for some bacteria in response to the environment stress, such as the Vibrio genus (Griffitt et al, 2011;Zhong et al 2016;Chahorm and Prakitchaiwattana 2018) or a stable state, as occurs in Mycobacterium leprae and Treponema pallidum (Petricha et al 2015;Ruiz-Fuentesa et al 2015;Gogarten et al 2016;Steinmann et al 2017). Thus, in both cases, applying molecular biology tools allows for their taxonomic classification, for the evaluation of their pathogenic potential and serve as a diagnostic method (Bergholz et al 2014;Das et al 2014;Kergourlay et al 2015;Sciuto and Moro 2015;King et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Application of molecular tools to elucidate the microbiota of seafood

2018

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The aim of this review is to present the methodologies currently applied to identify microbiota and pathogens transmitted to humans through seafood consumption, focusing on molecular techniques and pointing out their importance, advantages, disadvantages and applicability. Knowledge of available techniques allows researchers to identify which technique best fits their expectations. With such discernment, it will be possible to infer which disadvantages will be present and, therefore, not interfering with the final result. Two methodologies can be employed for this purpose, dependent and independent cultures. However, the dependent culture has certain limitations that can be solved through the independent cultivation techniques, such as PCR, PFGE and NGS, especially through the sequencing of the 16S rRNA region, providing a complete view of microbial diversity. These have revolutionized microbiological knowledge, mainly because they allow for the identification of uncultivable micro-organisms, which represent a substantial portion of total micro-organisms, making it possible to elucidate not yet described taxa which may display pathogenic potential, besides quantifying microbial communities, microbiota genetics, translated proteins and produced metabolites. In addition, transcriptomic and metabolomic techniques also allow for the evaluation of possible impacts that microbial communities may create in their environment, as well as the determination of potential pathogenicity to humans.

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Fluorescence in situ hybridization for the identification of Treponema pallidum in tissue sections

Cited by 17 publications

References 43 publications

Microbiological diagnosis of device‐related biofilm infections

Microbiological diagnosis of device‐related biofilm infections

Vaccine development for syphilis

Application of molecular tools to elucidate the microbiota of seafood

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