Fluorescence in situ hybridisation analysis of formalin-fixed paraffin-embedded tissue sections in the diagnostic work-up of non-Burkitt high grade B-cell non-Hodgkin's lymphoma: a single centre's experience

Abstract

FISH analysis of formalin-fixed paraffin-embedded tissue sections is a highly reproducible technique with an excellent success rate for the detection of genetic abnormalities which will play an increasingly important role in improving risk stratification of patients with HG-BNHL.

MYC-IG rearrangements are negative predictors of survival in DLBCL patients treated with immunochemotherapy: a GELA/LYSA study

“…We observed an overall incidence of MYC-R of 8.9%, which is consistent with the literature by using a commercially available probe that covers most breakpoints commonly involved in MYC translocations, and similar to the majority of previous published series. 10,[17][18][19]34,39,40 We corroborate previous reports on the adverse prognostic impact of MYC-R in DLBCL, which remained an independent prognostic factor for OS in multivariate analysis incorporating IPI and the Hans score. [4][5][6][7][8][9][10]14,22 However, we show that this prognostic impact is related to the MYC-Ig partner gene.…”

“…16,17 In contrast to BL where MYC is classically translocated within the immunoglobulin (IG) loci, either the IG heavy chain gene (IGH) or more rarely the Ig light chain genes k (IGK) or l (IGL), up to half of MYC-R DLBCL involve non-IG translocation partner genes including BCL6, PAX5, BCL11A, or IKAROS. [17][18][19][20][21] When considering the clinical relevance of MYC-R in DLBCL, there remain several unresolved issues. First, many groups have focused on the prognostic value of MYC gene deregulation in a variety of "aggressive" B-cell lymphomas with controversial results.…”

Diagnosis of ‘double hit’ diffuse large B-cell lymphoma and B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and Burkitt lymphoma: when and how, FISH versus IHC

“…2,3 At the present time, most use the term to refer to lymphomas with MYC plus BCL2 and/or, in ϳ30% of the cases in the recent literature, BCL6 rearrangements (R) with the even less common lymphomas bearing all 3 rearrangements considered "triple-hit" lymphomas (THLs). [4][5][6][7] The THLs are felt to be most similar to the BCL2 DHL cases. 8 Some have included cases that have MYC with other chromosomal translocations, such as BCL3 at 9p13, or even CCND1.…”

“…FISH studies can be successfully performed in almost all cases using formalin-fixed paraffin-embedded tissue sections, although smears, touch imprints, and fresh interphase or metaphase cells can also be used. 4 Reproducibility is reported to be very high, with concordance among 2 observers Ͼ98% and, in another study, complete agreement for breaks (kappa ϭ 1) and excellent agreement for gains (kappa ϭ 0.89). 5,7 Determination of the proportion of abnormal cells required for a positive result does vary somewhat between studies and can influence the overall results.…”

“…As defined in this way, 2%-12% of DLBCL (most studies Յ6%) and 32%-78% of BCLU are DHL, with the latter proportion being biased in some studies either because testing was performed on selected cases or because DH was used as a criterion for BCLU. [4][5][6][7][15][16][17][18][19][20][21][22][23][24][25] The incidence of DH/THL increases with patient age. 26 …”