Fluorescence in depth: integration of spectroscopy and imaging with Raman, IR, and CD for advanced research

Abstract

Fluorescence spectroscopy serves as a vital technique for studying the interaction between light and fluorescent 
molecules. It encompasses a range of methods, each presenting unique advantages and applications. This 
technique finds utility in various chemical studies. This review discusses Fluorescence spectroscopy, its branches 
such as Time-Resolved Fluorescence Spectroscopy (TRFS) and Fluorescence Lifetime Imaging Microscopy 
(FLIM), and their integration with other spectroscopic methods, including Raman, Infrared (IR), and Circular 
Dichroism (CD) spectroscopies. By delving into these methods, we aim to provide a comprehensive 
understanding of the capabilities and significance of fluorescence spectroscopy in scientific research, highlighting 
its diverse applications and the enhanced understanding it brings when combined with other spectroscopic 
methods. This review looks at each technique's unique features and applications. It discusses the prospects of their 
combined use in advancing scientific understanding and applications across various domains.