Fluorescence Imaging of Water and Temperature Stress in Plant Leaves

Applications of chlorophyll fluorescence imaging technique in horticultural research: A review

“…For example, Lang et al (1996) measured a linear increase of the fluorescence ratios F440/F690 and F440/F740 as the relative water content of tobacco leaves dropped below 88-84%, and they were able to differentiate local disturbances in these ratios in the complete leaf area. Lichtenthaler and Babani (2000) and Lichtenthaler et al (2005b) could also measure gradients in photosynthetic capacity over different parts of bean leaves by CFI.…”

“…High temperature stress can often develop together with water stress. Lang et al (1996) measured the simultaneous effect of both stresses in tobacco leaves and showed a gradient of Chl fluorescence emission. There was a significant increase of red (F690) and far-red (F740) chlorophyll fluorescence emission, and a decrease of the ratios F440/F690 and F440/F740 on the leaf rim, whereas the central part of the leaf still exhibited the regular fluorescence signatures of photosynthetically active leaves.…”

Investigation of Leaf Diseases and Estimation of Chlorophyll Concentration in Seven Barley Varieties Using Fluorescence and Hyperspectral Indices

“…Blue/red (BF/RF) and blue/far-red (BF/FRF) fluorescence ratios and combined fluorescence indices also allow to detect various stresses [8,30] such as water [9] and nitrogen (N) deficiencies [11,31,32] and to monitor changes in chlorophyll and polyphenols [11,33]. However, studies on detecting cereal diseases or estimating chlorophyll content of barley plants are scarce.…”

Hartmut Lichtenthaler: an authority on chloroplast structure and isoprenoid biochemistry

“…Lichtenthaler was able to show that this was caused by air pollutants, the most detrimental of which was ozone Hartmut made contributions both to the detection of stress using chlorophyll a fluorescence (Schweiger et al 1996;Lichtenthaler et al 1998) and detecting variations in photosynthetic rate across leaves and canopies using chlorophyll fluorescence imaging (Fig. 3;Lang et al 1996;Lichtenthaler et al 1996;Lichtenthaler and Miehé 1997).…”