Fluorescence imaging for photodynamic therapy of non-melanoma skin malignancies – A retrospective clinical study

Gamayunov, S. V.; Turchin, Ilya V.; Fiks, I. I.; Корчагина, К. С.; Kleshnin, Mikhail; Шахова, Н. М.

doi:10.1515/plm-2015-0042

Cited by 13 publications

(17 citation statements)

References 35 publications

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“…This compound has unique acid-base properties and can be used as indicator of acidbase processes. It was shown by 1 H NMR spectroscopy that the target product did not form the expected tatraazachlorin similar structure.…”

Section: Discussionmentioning

confidence: 98%

“…Silica gel of 60 0.05-0.20 mm (MachereyNagel) was used for column chromatography. 10,17,12,19,k,p] (1,5,9,13)tetraazacyclohexadecene (1) was obtained by the reaction of 0.26 g (0.91 mmol) of 5-amino-2-dodecyl-3-imino-1,2,4-thiadiazoline (2), synthesized by method described in the literature, [6] and 0.4 g (2.74 mmol) of 1,3-diiminoisoindoline in 3 mL of phenol with step-by-step heating during 1.5-2 hours. The reaction mixture was stirred for 50 hours at temperature 100-110 °С, then the product was washed by hot water and hexane in Soxlet apparatus while absence of phenol, which was determined by qualitative reaction with ferric chloride.…”

Section: Methodsmentioning

confidence: 99%

“…In the Russian Federation, sensitizers for photodynamic therapy of oncological diseases have been approved, i.e. Fotoditazin, [1,2] Radahlorin, [3] Photolon, [4] which are water-soluble chlorin e 6 salts. The difficulty of synthesis of such compounds lies in the reduction of porphyrins, which results in the formation of a mixture of chlorins and bacteriochlorins.…”

Section: Introductionmentioning

confidence: 99%

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Macroheterocyclic Compound of ABBB-Type Containing 2N-Alkyl Substituted 1,2,4-Thiadiazoline Fragment: Synthesis and Acid-Base Properties

Butina¹,

Данилова²,

Kudayarova³

et al. 2018

MHC

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Macroheterocyclic Compound of ABBB-Type Containing 2N-Alkyl Substituted 1,2,4-Thiadiazoline Fragment: Synthesis and Acid-Base Properties

Butina¹,

Данилова²,

Kudayarova³

et al. 2018

MHC

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“…The achieved PE values confirm the efficiency of the performed procedure, since the level of 40% is typically considered as a success. 28 Averaged photobleaching efficiencies as the function of the delivered light dose at the wavelength of 405 and 660 nm are shown in Figs. 3(c) and 3(d), respectively.…”

Section: Fluorescence Monitoring Of the Photodynamic Therapy Proceduresmentioning

confidence: 99%

“…Two specific absorption peaks in combination with fluorescent properties of chlorin e6 PS provide additional diagnostic opportunities for fluorescence imaging (FI). FI is widely employed for monitoring the PS accumulation and distribution in biotissue prior to a PDT procedure 1,[27][28][29] and for evaluation of PS photobleaching in the course of the procedure. 30,31 Fluorescence excitation at two different wavelengths from the red and blue spectral ranges provides the opportunity to detect fluorescence response of PS from different depths depending on the penetration of the probing radiation into the tissue.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

Kurakina¹,

Khilov²,

Shakhova

et al. 2019

J. Biomed. Opt.

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Comparative analysis of single-and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study," J.Abstract. Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. We provide a comparative analysis of different PDT regimes performed with blue and red lights and their combination, with doses varying from 50 to 150 J∕cm 2 . The study was performed on the intact skin of a rabbit ear inner surface, with the use of chlorin e6 as a PS. PDT procedure protocol included monitoring of the treated site with fluorescence imaging technique to evaluate PS accumulation and photobleaching, as well as with optical coherence tomography (OCT) to register morphological and functional responses of the tissue. Optical diagnostic observations were compared with the results of histopathology examination. We demonstrated that PDT procedures with the considered regimes induce weaker organism reaction manifested by edema in normal tissue as compared to irradiation-only exposures with the same light doses. The light doses delivered with red light induce weaker tissue reaction as compared to the same doses delivered with blue light only or with a combination of red and blue lights in equal parts. Results of in-vivo OCT monitoring of tissue reaction are in agreement with the results of histopathology study.

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Optical coherence angiography for pre-treatment assessment and treatment monitoring following photodynamic therapy: a basal cell carcinoma patient study

Gubarkova

Feldchtein

Zagaynova

et al. 2019

Sci Rep

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Microvascular networks of human basal cell carcinomas (Bcc) and surrounding skin were assessed with optical coherence angiography (ocA) in conjunction with photodynamic therapy (pDt). ocA images were collected and analyzed in 31 lesions pre-treatment, and immediately/24 hours/3-12 months posttreatment. Pre-treatment OCA enabled differentiation between prevalent subtypes of BCC (nodular and superficial) and nodular-with-necrotic-core BCC subtypes with a diagnostic accuracy of 78%; this can facilitate more accurate biopsy reducing sampling error and better therapy regimen selection. Post-treatment OCA images at 24 hours were 98% predictive of eventual outcome. Additional findings highlight the importance of pre-treatment necrotic core, vascular metrics associated with hypertrophic scar formation, and early microvascular changes necessary in both tumorous and peri-tumorous regions to ensure treatment success. Basal cell carcinoma (BCC) is the most common slow-growing locally invasive malignant epidermal skin tumor, with majority of the lesions localized on the head and neck 1. There are many different classification schemes for the BCC types and subtypes including nodular, superficial, infundibulocystic, fibroepithelial and the aggressive growth variants such as infiltrative, micronodular and morpheaform (sclerosing) plus some combinations 2,3. BCC treatment management starts with accurate diagnosis, including histopathology 4,5 and the development of reliable and non-invasive techniques for its diagnosis is crucial. Currently such non-invasive technologies have limited reliability and more development and validation work is needed 6. Dermatoscopy is a useful tool in the preoperative prediction of the BCC subtype 7. However, the evidence of its clinical diagnostic efficacy is somewhat limited and in ambiguous lesions, the BCC subtype has to be assessed histopathologically 8. Modern optical imaging modalities such as wide-field fluorescence imaging 9 , confocal microscopy 10 , optical coherence tomography (OCT) 11-13 , polarization-sensitive OCT 14,15 , OCT-based angiography (OCA) 16,17 and photoacoustic imaging 18 and their combinations 19,20 offer new possibilities for detection and visualization of human skin tumor structure and microvascular network in real time. OCT is particularly promising as it offers non-invasive structural and functional 3D imaging of biological tissues with high spatial resolution 11 , in particular for BCC 9,13,21. For the first time in this study, we used spectral domain OCT/OCA system to assess the after-treatment effects following photodynamic therapy (PDT) in patients with different BCC subtypes. PDT involves administration of photosensitizes which preferentially accumulate in malignant tissues, followed by local light activation at appropriate wavelength, producing oxygen free radicals and singlet oxygen 22. The generation of these reactive oxygen species subsequently causes tumor cell death by apoptosis, necrosis

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Fluorescence imaging for photodynamic therapy of non-melanoma skin malignancies – A retrospective clinical study

Cited by 13 publications

References 35 publications

Macroheterocyclic Compound of ABBB-Type Containing 2N-Alkyl Substituted 1,2,4-Thiadiazoline Fragment: Synthesis and Acid-Base Properties

Macroheterocyclic Compound of ABBB-Type Containing 2N-Alkyl Substituted 1,2,4-Thiadiazoline Fragment: Synthesis and Acid-Base Properties

Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

Optical coherence angiography for pre-treatment assessment and treatment monitoring following photodynamic therapy: a basal cell carcinoma patient study

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