Fluorescence imaging analysis of distribution of indocyanine green in molecular and nanoform in tumor model

Farrakhova, Dina; Maklygina, Yulia; Romanishkin, I.D.; Yakovlev, Dmitry; Plyutinskaya, A. D.; Bezdetnaya, Lina; Loschenov, Victor B.

doi:10.1016/j.pdpdt.2021.102636

Cited by 13 publications

(10 citation statements)

References 29 publications

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“…Nowadays, as an objective, non-invasive and real-time approach, near infrared imaging has attracted much attention [23][24][25][26]. Previous studies have shown that it has been broadly used in lymphangiography, angiography, sentinel lymph nodes, tumor margin detection and so on [27][28][29]. More importantly, these results also show that NIR-II imaging has advantages over traditional NIR-I imaging in clinical scenarios based on its advantages in depth sensitivity, higher spatial resolution and improved signal-to-noise ratio [8,12,13,23].…”

Section: Discussionmentioning

confidence: 99%

NIR‐II imaging with ICG for identifying perforators, assessing flap status and predicting division timing of pedicled flaps in a porcine model

Wang

et al. 2022

Journal of Biophotonics

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The use of skin flaps to fill large defects is a key surgical technique in reconstructive surgery, effective real‐time in vivo imaging for flap design and use is urgent. Currently, fluorescent imaging in the second NIR window (NIR‐II; 1000‐1700 nm) is characterized by non‐radiation, less expensive and higher resolution in comparisons with the first NIR window (NIR‐I; 700‐900 nm) and other traditional imaging modalities. In this article, we identified the location and numbers of perforators and choke zone via NIR‐II imaging. Then, eight abdominal perforator flaps were established and the perfusion zones were evaluatedat special time points. Finally, after eight pedicled flaps establishment, NIR‐II imaging was used to guide the optimal timing for division of flap pedicle. The results showed that NIR‐II fluorescence imaging with indocyanine green (ICG) can reliably visualize vascular supply, which makes it to be an accurate and in vivo imaging approach to flap clinical design and use.

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Section: Discussionmentioning

confidence: 99%

NIR‐II imaging with ICG for identifying perforators, assessing flap status and predicting division timing of pedicled flaps in a porcine model

Wang

et al. 2022

Journal of Biophotonics

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“…Furthermore, its fluorescence decayed in blood within 5 h, and ICGJ dissociations have been observed during cellular internalization in vivo . After intravenous injection of ICGJ with sizes 100–400 nm in a murine tumor xenograft model, the fluorescent signal for ICGJ was detected primarily in the liver and spleen, which suggests elimination of ICGJ by the reticulo-endothelial system within 24 h of injection.…”

Section: Introductionmentioning

confidence: 99%

“…20,21 Furthermore, ICG undergoes rapid clearance from the circulatory system in vivo. 22 To overcome these pharmacokinetic limitations, ICG has been stabilized by encapsulating into liposomes, 23 polymeric, 24 and other kinds of NPs, as reviewed recently. 25 Unfortunately, the maximum absorbance of ICG at 785 nm overlaps with the hemoglobin absorption in the NIR-I window and thus strongly interferes with functional PAI.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Indocyanine green (ICG) is one of the very few U.S. FDA-approved exogenous contrast agents in the NIR region for clinical applications, having received approval in 1975. , However, 95 and 4% of free ICG molecules strongly bind to albumin and lipoproteins in blood plasma, respectively, for a typical dosage. , Furthermore, ICG undergoes rapid clearance from the circulatory system in vivo . To overcome these pharmacokinetic limitations, ICG has been stabilized by encapsulating into liposomes, polymeric, and other kinds of NPs, as reviewed recently .…”

Section: Introductionmentioning

confidence: 99%

“…Further, ICGJ has much greater photostability against photobleaching than ICG and is much more soluble in aqueous solutions. While unencapsulated ICGJ has been used for PAI, , it has been found to dissociate in fetal bovine serum within 20 min, as demonstrated by dynamic light scattering. Furthermore, its fluorescence decayed in blood within 5 h, and ICGJ dissociations have been observed during cellular internalization in vivo .…”

Section: Introductionmentioning

confidence: 99%

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Antibody-Conjugated Polymersomes with Encapsulated Indocyanine Green J-Aggregates and High Near-Infrared Absorption for Molecular Photoacoustic Cancer Imaging

Kawelah,

Han,

Atila Dincer

et al. 2024

ACS Appl. Mater. Interfaces

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Imaging plays a critical role in all stages of cancer care from early detection to diagnosis, prognosis, and therapy monitoring. Recently, photoacoustic imaging (PAI) has started to emerge into the clinical realm due to its high sensitivity and ability to penetrate tissues up to several centimeters deep. Herein, we encapsulated indocyanine green J (ICGJ) aggregate, one of the only FDA-approved organic exogenous contrast agents that absorbs in the near-infrared range, at high loadings up to ∼40% w/w within biodegradable polymersomes (ICGJ-Ps) composed of poly(lactide-co-glycolide-b-polyethylene glycol) (PLGA-b-PEG). The small Ps hydrodynamic diameter of 80 nm is advantageous for in vivo applications, while directional conjugation with epidermal growth factor receptor (EGFR) targeting cetuximab antibodies renders molecular specificity. Even when exposed to serum, the ∼11 nm-thick membrane of the Ps prevents dissociation of the encapsulated ICGJ for at least 48 h with a high ratio of ICGJ to monomeric ICG absorbances (i.e., I 895 /I 780 ratio) of approximately 5.0 that enables generation of a strong NIR photoacoustic (PA) signal. The PA signal of polymersome-labeled breast cancer cells is proportional to the level of cellular EGFR expression, indicating the feasibility of molecular PAI with antibodyconjugated ICGJ-Ps. Furthermore, the labeled cells were successfully detected with PAI in highly turbid tissue-mimicking phantoms up to a depth of 5 mm with the PA signal proportional to the amount of cells. These data show the potential of molecular PAI with ICGJ-Ps for clinical applications such as tumor margin detection, evaluation of lymph nodes for the presence of micrometastasis, and laparoscopic imaging procedures.

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Visualization of Blood–Brain Barrier Disruption in Septic Mice with the New Method Based on in Vivo Imaging Technology

Zhang,

Ai,

Zhang

et al. 2024

Neurocrit Care

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Fluorescence imaging analysis of distribution of indocyanine green in molecular and nanoform in tumor model

Cited by 13 publications

References 29 publications

NIR‐II imaging with ICG for identifying perforators, assessing flap status and predicting division timing of pedicled flaps in a porcine model

NIR‐II imaging with ICG for identifying perforators, assessing flap status and predicting division timing of pedicled flaps in a porcine model

Antibody-Conjugated Polymersomes with Encapsulated Indocyanine Green J-Aggregates and High Near-Infrared Absorption for Molecular Photoacoustic Cancer Imaging

Visualization of Blood–Brain Barrier Disruption in Septic Mice with the New Method Based on in Vivo Imaging Technology

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