Fluorescence hybridization chain reaction enables localization of multiple molecular classes combined with plant cell ultrastructure
Yunqing Yu,
David Huss,
Mao Li
et al.
Abstract:Recent developments in hybridization chain reaction (HCR) have enabled robust simultaneous localization of multiple mRNA transcripts using fluorescence in situ hybridization (FISH). Once multiple split initiator oligonucleotide probes bind their target mRNA, HCR uses DNA base-pairing of fluorophore-labeled hairpin sets to self-assemble into large polymers, amplifying the fluorescence signal and reducing non-specific background. Few studies have applied HCR in plants, despite its demonstrated utility in whole m… Show more
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