Fluorescence-guided management of deep endometriosis

Aleksandrov, Atanas; Meshulam, Moshe; Smith, Andres Vigueras; Chauvet, Pauline; Canis, M; Bourdel, Nicolas

doi:10.1016/j.fertnstert.2020.07.026

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…If desired, these last two markers could help discriminate infiltrating tumor tissue with FGS, due to the absence of both markers in surrounding stromal tissue. Alternatively, these markers may be used for reversed fluorescence imaging, with distinctive higher expression of the target in healthy compared with malignant tissue [ 35 ]. MUC1 and uPAR were excluded from further evaluation due to their heterogenous or patchy expression pattern in vulvar malignant tissue.…”

Section: Discussionmentioning

confidence: 99%

Integrin αvβ6 as a Target for Tumor-Specific Imaging of Vulvar Squamous Cell Carcinoma and Adjacent Premalignant Lesions

Huisman

Cankat

Bosse

et al. 2021

Cancers

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Surgical removal of vulvar squamous cell carcinoma (VSCC) is associated with significant morbidity and high recurrence rates. This is at least partially related to the limited visual ability to distinguish (pre)malignant from normal vulvar tissue. Illumination of neoplastic tissue based on fluorescent tracers, known as fluorescence-guided surgery (FGS), could help resect involved tissue and decrease ancillary mutilation. To evaluate potential targets for FGS in VSCC, immunohistochemistry was performed on paraffin-embedded premalignant (high grade squamous intraepithelial lesion and differentiated vulvar intraepithelial neoplasia) and VSCC (human papillomavirus (HPV)-dependent and -independent) tissue sections with healthy vulvar skin as controls. Sections were stained for integrin αvβ6, CAIX, CD44v6, EGFR, EpCAM, FRα, MRP1, MUC1 and uPAR. The expression of each marker was quantified using digital image analysis. H-scores were calculated and percentages positive cells, expression pattern, and biomarker localization were assessed. In addition, tumor-to-background ratios were established, which were highest for (pre)malignant vulvar tissues stained for integrin αvβ6. In conclusion, integrin αvβ6 allowed for the most robust discrimination of VSCCs and adjacent premalignant lesions compared to surrounding healthy tissue in immunohistochemically stained tissue sections. The use of an αvβ6 targeted near-infrared fluorescent probe for FGS of vulvar (pre)malignancies should be evaluated in future studies.

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Section: Discussionmentioning

confidence: 99%

Integrin αvβ6 as a Target for Tumor-Specific Imaging of Vulvar Squamous Cell Carcinoma and Adjacent Premalignant Lesions

Huisman

Cankat

Bosse

et al. 2021

Cancers

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“…Therefore, improvements in the resection technique are highly sought to improve outcomes, decrease functional loss and recurrence, and increase the patient's quality of life. Minimally invasive surgery is the preferred surgical approach because it is usually associated with less pain, shorter hospital stays, and faster recovery [7].…”

Section: Introductionmentioning

confidence: 99%

“…Although endoscopy allows for magnification of the operative field, the identification of endometriosis implants is not always possible using white light because some implants may be very small or hidden, especially in case of DIE covered by healthy peritoneum or small superficial implant extending deeply retroperitoneum. Studies report that enhanced imaging allows for detecting of additional endometriotic lesions missed by conventional white-light laparoscopy [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, improvements in the resection technique are highly sought to improve outcomes, decrease functional loss and recurrence, and increase the patient’s quality of life. Minimally invasive surgery is the preferred surgical approach because it is usually associated with less pain, shorter hospital stays, and faster recovery [7]. Although endoscopy allows for magnification of the operative field, the identification of endometriosis implants is not always possible using white light because some implants may be very small or hidden, especially in case of DIE covered by healthy peritoneum or small superficial implant extending deeply retroperitoneum.…”

Section: Introductionmentioning

confidence: 99%

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PARP-1, EpCAM, and FRα as potential targets for intraoperative detection and delineation of endometriosis: a quantitative tissue expression analysis

Belmonte,

Di Lorenzo,

Mangogna

et al. 2023

Preprint

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Endometriosis is a gynecological disease characterized by the presence of endometrial tissue in abnormal locations, leading to severe symptoms, inflammation, pain, organ dysfunction, and infertility. Surgical removal of endometriosis lesions is crucial for improving pain and fertility outcomes, with the goal of complete lesion removal. Incomplete surgery often results in recurring symptoms and the need for additional interventions. This study aims to explore the biological significance of PARP-1, EpCAM, and FRα as potential targets for molecular imaging in endometriosis, assessing their suitability for targeted intraoperative imaging.Gene expression analysis was performed using the EndometDB. By immunohistochemistry, we investigated the presence and distribution of PARP-1, EpCAM, and FRα in endometriosis foci and adjacent tissue. We also applied an ad hoc platform for the analysis image to perform a quantitative immunolocalization analysis. Additionally, double immunofluorescence analysis was carried out for PARP-1 and EpCAM, as well as PARP-1 and FRα, to explore the expression of these combined markers within endometriosis foci and their potential simultaneous utilization in surgical treatment.The analysis of gene expression revealed that PARP-1, EpCAM, and FRα exhibit higher levels of expression in endometriotic lesions compared to the peritoneum, used for control tissue. Immunohistochemical analysis also highlighted a significant increase in expression of all three biomarkers in endometriosis foci compared to surrounding tissues, as well as the quantitative analysis of immunolocalization of the signal. Furthermore, the double immunofluorescence analysis consistently revealed nuclear expression of PARP-1 and membrane and cytoplasmatic expression of EpCAM and FRα, although relatively weaker.Overall, the three markers demonstrate significant potential for effective imaging of endometriosis. Particularly, the results emphasize the importance of PARP-1 expression as a possible indicator for distinguishing endometriotic lesions from adjacent tissue. PARP-1, as a potential biomarker for endometriosis, offers promising avenues for further investigation in terms of both pathophysiology and diagnostic-therapeutic approaches.

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Instrumentation, nouvelles énergies et fluorescence

Chauvet¹

2022

Chirurgie De l'Endométriose

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Fluorescence-guided management of deep endometriosis

Cited by 8 publications

References 11 publications

Integrin αvβ6 as a Target for Tumor-Specific Imaging of Vulvar Squamous Cell Carcinoma and Adjacent Premalignant Lesions

Integrin αvβ6 as a Target for Tumor-Specific Imaging of Vulvar Squamous Cell Carcinoma and Adjacent Premalignant Lesions

PARP-1, EpCAM, and FRα as potential targets for intraoperative detection and delineation of endometriosis: a quantitative tissue expression analysis

Instrumentation, nouvelles énergies et fluorescence

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