Fluorescence excitation spectrum of organic compounds in solution. Part 1.—Systems with quantum yield independent of the exciting wavelength

Weber, Gregorio; Teale, F. W. J.

doi:10.1039/tf9585400640

Cited by 204 publications

(74 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, acridine orange in organic glasses and in EtOH at 77 K [22,23], rhodamine dyes Rh-6G [7], Rh-19 [24], and Rh-B [25] in ethanolic and aqueous solutions, Cresyl Violet in water [26] form aggregates. For fluorescein-Na, in alkaline aqueous solutions at 1 Â 10 À1 mol dm À3 , dimeric species has been reported [21].…”

Section: Article In Pressmentioning

confidence: 99%

Dual fluorescence and laser emissions from fluorescein-Na and eosin-B

Math

Naik

Suresh

et al. 2006

Journal of Luminescence

View full text Add to dashboard Cite

Section: Article In Pressmentioning

confidence: 99%

Dual fluorescence and laser emissions from fluorescein-Na and eosin-B

Math

Naik

Suresh

et al. 2006

Journal of Luminescence

View full text Add to dashboard Cite

“…The efficiency of energy transfer is readily obtainable from the excitation spectrum of the energy acceptor (20,21): at a given wavelength, the magnitude of the excitation spectrum of the energy acceptor (I) can be determined by the expression I = ε A + Eε D , where E is the transfer efficiency and ε D and ε A are the extinction coefficients of the energy donor and energy acceptor, respectively. When E = 0% (i.e., no FRET), the excitation spectrum is identical to the absorption spectrum of the energy acceptor.…”

Section: And Tgtmentioning

confidence: 99%

Fluorescence resonance energy transfer from pyrene to perylene labels for nucleic acid hybridization assays under homogeneous solution conditions

Masuko

Ohuchi²,

Sode³

et al. 2000

Nucleic Acids Research

View full text Add to dashboard Cite

We characterized the fluorescence resonance energy transfer (FRET) from pyrene (donor) to perylene (acceptor) for nucleic acid assays under homogeneous solution conditions. We used the hybridization between a target 32mer and its complementary two sequential 16mer deoxyribonucleotides whose neighboring terminals were each respectively labeled with a pyrene and a perylene residue. A transfer efficiency of~100% was attained upon the hybridization when observing perylene fluorescence at 459 nm with 347-nm excitation of a pyrene absorption peak. The Förster distance between two dye residues was 22.3 Å (the orientation factor of 2/3). We could change the distance between the residues by inserting various numbers of nucleotides into the center of the target, thus creating a gap between the dye residues on a hybrid. Assuming that the number of inserted nucleotides is proportional to the distance between the dye residues, the energy transfer efficiency versus number of inserted nucleotides strictly obeyed the Förster theory. The mean inter-nucleotide distance of the single-stranded portion was estimated to be 2

show abstract

“…In general, the fluorescence and phosphorescence quantum yields of arorllatic molecules clo not depend on which electronic state of the molecule is initially excited (6,8,9,13,25). I n Part I of this series (9) seine exceptional cases were reported and it was shown that this is not ilecessarily true if the excited illolecule is free to isomerize.…”

mentioning

confidence: 94%

The Luminescence of Complex Molecules in Relation to the Internal Conversion of Excitation Energy: Part Ii. N-Heteroaromatics

Hochstrasser¹

1960

Can. J. Chem.

View full text Add to dashboard Cite

The relative fluorescence and/or phosphorescence yields of a n~~~n b e r of methyl-and halogeno-qui~~olines, isoq~~inolil~es, and acridines have been nleasured a s a f~~n c t i o n of the wavelength of the exciting light. I n all cases the solvent was absolute ethanol. The r e s~~l t sshow that the efficiency of internal conversion processes between excited singlet states is extremely high in comparison to other intramolecular steps. The effect of s~l b s t i t~~t e d heavy atoms is discussed, as well as the fluorescence of the conjugate acids of the bases.

show abstract

Fluorescence excitation spectrum of organic compounds in solution. Part 1.—Systems with quantum yield independent of the exciting wavelength

Cited by 204 publications

References 1 publication

Dual fluorescence and laser emissions from fluorescein-Na and eosin-B

Dual fluorescence and laser emissions from fluorescein-Na and eosin-B

Fluorescence resonance energy transfer from pyrene to perylene labels for nucleic acid hybridization assays under homogeneous solution conditions

The Luminescence of Complex Molecules in Relation to the Internal Conversion of Excitation Energy: Part Ii. N-Heteroaromatics

Contact Info

Product

Resources

About