Fluorescence-excitation spectra of acridine orange-DNA and -RNA systems

Tomita, Giiti

doi:10.1007/bf01188503

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…Although the efficiency of photovoltaic devices could be in principle improved by mixing AO with water-soluble pyrrolidinium-type fullerene derivatives 23 with the formation of bulk heterojunction device structures, the crystallographic restructure of AO in these systems would be difficult to predict and the biosensitivity of these blends is questionable as well. It is well known that interaction with nucleic acids changes the photoactivity of acridine orange 13,14 and subsequently the band gap and the photo-induced conduction band population (n) in these materials. Our study demonstrates that acridine orange has the unique capability to be employed in fabrication of biosensitive photovoltaic devices.…”

Section: Resultsmentioning

confidence: 99%

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Acridine orange as a biosensitive photovoltaic material

Sharifi

Bauld

Fanchini

2013

Journal of Applied Physics

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Section: Resultsmentioning

confidence: 99%

“…Importantly, AO is a bio-sensitive material that changes its properties when it interacts with nucleic acids. 13,14 Our study is aimed at demonstrating the potential of AO in photo-biosensing applications.…”

Section: Introductionmentioning

confidence: 99%

Acridine orange as a biosensitive photovoltaic material

Sharifi

Bauld

Fanchini

2013

Journal of Applied Physics

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“…Bulk measurement results (summarized in Table 1) clearly indicate that the emission peak of 530 nm (green, 10 −7 M AO) is associated with FLT of 2 nsec and the emission of [26][27][28].…”

Section: Wo-flt Versus Flim Measurements Of Ao Stained U937 Cellsmentioning

confidence: 95%

Whole-Object Fluorescence Lifetime Setup for Efficient Non-Imaging Quantitative Intracellular Fluorophore Measurements

et al. 2012

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In the present study we introduce a Whole-Object Fluorescence Life Time (wo-FLT) measurement approach for ease and a relatively inexpensive method of tracing alterations in intracellular fluorophore distribution and in the physical-chemical features of the microenvironments hosting the fluorophore. Two common fluorophores, Rhodamine 123 and Acridine Orange, were used to stain U937 cells which were incubated, with and without either Carbonyl cyanide 3-chlorphenylhydrazon or the apoptosis inducer H(2)O(2). The wo-FLT, which is a non-imaging quantitative measurement, was able to detect several fluorescence decay components and corresponding weights in a single cell resolution. Following cell treatment, both decay time and weight were altered. Results suggest that the prominent factor responsible for these alterations and in some cases to a shift in emission spectrum as well, is the intracellular fluorophore local concentration. In this study it was demonstrated that the proposed wo-FLT method is superior to color fluorescence based imaging in cases where the emission spectrum of a fluorophore remains unchanged during the investigated process. The proposed wo-FLT approach may be of particular importance when direct imaging is impossible.

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“…acridine orange (Loeser et al, 1960;Boyle et al, 1962;Borisova and Tumerman, 1964;Weill and Calvin, 1965;Lober, 1965;Tomita, 1967a;1967b;Lober, 1968;Borisova and Minyat, 1969;Killander and Rigler, 1969;Lober and Achtert, 1969;Yamabe, 1969;Kubota, 1970a;1970b;Romanovskaja et al, 1972), the alkaloid berberine (Hahn and Krey. 1971) and the trypanocidal dye ethidium bromide (Le Pecq and Paoletti, 1967;Sela, 1969;Maelicke, 1970;Rigler, 1970).…”

Section: Furocoumarinsmentioning

confidence: 99%